标签归档：taquest

TAQuest qPCR Master Mix with Helixyte Green *低ROX* 货号17272-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX* 货号17272 货号 17272 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17272

产品名称：TAQuest qPCR Master Mix with Helixyte Green *低ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *低ROX*。 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *低ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *低 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

A new SYBR Green real-time PCR to detect SARS-CoV-2.
Authors: Marinowic, D R and Zanirati, G and Rodrigues, F V F and Grahl, M V C and Alcará, A M and Machado, D C and Da Costa, J C
Journal: Scientific reports (2021): 2224

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Authors: Abdel Sater, Fadil and Younes, Mahmoud and Nassar, Hassan and Nguewa, Paul and Hamze, Kassem
Journal: Molecular biology reports (2021): 7243-7249

Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer.
Authors: Desriani and Azamris and Ghaissani, Shabrina S and Kinanti, Senja R and Warisman, Muhammad A and Fitria, N
Journal: Journal, genetic engineering & biotechnology (2021): 6

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.
Authors: Wang, Yong and Li, Wei and Guo, Xu and Zhang, Da and Sun, Jianfei and Fu, Ziteng and Liu, Guangqing and Li, Yongdong and Jiang, Shudong
Journal: 3 Biotech (2021): 61

TAQuest qPCR Master Mix with Helixyte Green *低ROX* 货号17273-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX*

TAQuest qPCR Master Mix with Helixyte Green *低ROX* 货号17273 货号 17273 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17273

产品名称：TAQuest qPCR Master Mix with Helixyte Green *低ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *低ROX*。 

 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *低ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *低 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

A SYBR Green I-based real-time polymerase chain reaction assay for detection and quantification of canine bufavirus.
Authors: Wang, Yong and Sun, Jianfei and Guo, Xu and Li, Wei and Zhang, Da and Liu, Guangqing and Zhou, Tianhong and Li, Yongdong
Journal: Molecular and cellular probes (2021): 101762

A duplex SYBR green I-based real-time polymerase chain reaction assay for concurrent detection of feline parvovirus and feline coronavirus.
Authors: Sun, Liting and Xu, Zhiqing and Wu, Junhuang and Cui, Yongqiu and Guo, Xu and Xu, Fazhi and Li, Yongdong and Wang, Yong
Journal: Journal of virological methods (2021): 114294

A new SYBR Green real-time PCR to detect SARS-CoV-2.
Authors: Marinowic, D R and Zanirati, G and Rodrigues, F V F and Grahl, M V C and Alcará, A M and Machado, D C and Da Costa, J C
Journal: Scientific reports (2021): 2224

A novel duplex SYBR Green real-time PCR with melting curve analysis method for beef adulteration detection.
Authors: Li, Jiapeng and Wei, Yixuan and Li, Jinchun and Liu, Ruixi and Xu, Suigen and Xiong, Suyue and Guo, Ya and Qiao, Xiaoling and Wang, Shouwei
Journal: Food chemistry (2021): 127932

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.
Authors: Abdel Sater, Fadil and Younes, Mahmoud and Nassar, Hassan and Nguewa, Paul and Hamze, Kassem
Journal: Molecular biology reports (2021): 7243-7249

Design and characterization of a SYBR Green I-based melting curve method for investigation of HER2I655V polymorphism in breast cancer.
Authors: Desriani and Azamris and Ghaissani, Shabrina S and Kinanti, Senja R and Warisman, Muhammad A and Fitria, N
Journal: Journal, genetic engineering & biotechnology (2021): 6

Development and Validation of a SYBR Green Real Time PCR Protocol for Detection and Quantification of Nervous Necrosis Virus (NNV) Using Different Standards.
Authors: Olveira, José G and Souto, Sandra and Bandín, Isabel and Dopazo, Carlos P
Journal: Animals : an open access journal from MDPI (2021)

Development and application of SYBR Green Ⅰ real-time quantitative reverse transcription PCR assay for detection of swine Getah virus.
Authors: Xia, Yin-He and Shi, Zi-Cong and Wang, Xin-Wei and Li, Yong-Tao and Wang, Zeng and Chang, Hong-Tao and Liu, Hong-Ying and Chen, Lu and Wang, Chuan-Qing and Yang, Xia
Journal: Molecular and cellular probes (2021): 101730

Development of New PCR Assay with SYBR Green I for Detection of Mycoplasma, Acholeplasma, and Ureaplasma sp. in Cell Cultures.
Authors: Krzysztoń-Russjan, Jolanta and Chudziak, Jakub and Bednarek, Małgorzata and Anuszewska, Elżbieta Lidia
Journal: Diagnostics (Basel, Switzerland) (2021)

Development of SYBR Green I-based polymerase chain reaction for feline bocavirus 1 detection.
Authors: Wang, Yong and Li, Wei and Guo, Xu and Zhang, Da and Sun, Jianfei and Fu, Ziteng and Liu, Guangqing and Li, Yongdong and Jiang, Shudong
Journal: 3 Biotech (2021): 61

TAQuest qPCR Master Mix with Helixyte Green *高ROX* 货号17274-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

货号 17274 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17274

产品名称：TAQuest qPCR Master Mix with Helixyte Green *高ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含大量ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *高ROX*。 

 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *高ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *高 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Aligned Expression of IFI16 and STING Genes in RRMS Patients’ Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Authors: Siddiqi, Allauddin and Milne, Trudy and Cullinan, Mary P and Seymour, Gregory J
Journal: Clinical oral implants research (2016): 288-94

Real-time immuno-PCR for ultrasensitive detection of pyrene and other homologous PAHs.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Zhang, Y Y and Qiao, B and Sun, Y and Yang, L and Hu, P and Lu, S Y and Ren, H L and Zhang, J H and Wang, X R and Liu, Z S
Journal: Biosensors & bioelectronics (2015): 42-7

Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Authors: Ramos, Carlos A N and Araújo, Flábio R and Souza, Ingrid I F and Bacanelli, G and Luiz, Hera L and Russi, Lívia S and Oliveira, Renato H M and Soares, Cleber O and Rosinha, Grácia M S and Alves, Leucio C
Journal: Veterinary parasitology (2011): 79-83

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17276-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17276 货号 17276 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17276

产品名称：TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest FAST qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化。对于 20 uL 反应体积中的 40 个 PCR 循环，预混液可在 50 分钟内提供结果。该混合物包括含有 dNTP 的优化缓冲液和我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶，该酶旨在允许即时热启动，最大限度地减少非特异性产物的形成，从而允许室温反应设置。运行所需的 PCR 反应只需要模板和目标引物。 TAQuest FAST qPCR Master Mix 与 Helixyte Green 确保 PCR 特异性和灵敏度对所有样品类型，如基因组、质粒、病毒和 cDNA 模板。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*。 

 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无ROX* 解冻 TAQuest™ FAST qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D’Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7

Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6

Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine: 1234-8

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17277-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17277 货号 17277 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17277

产品名称：TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest FAST qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化。对于 20 uL 反应体积中的 40 个 PCR 循环，预混液可在 50 分钟内提供结果。该混合物包括含有 dNTP 的优化缓冲液和我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶，该酶旨在允许即时热启动，最大限度地减少非特异性产物的形成，从而允许室温反应设置。运行所需的 PCR 反应只需要模板和目标引物。 TAQuest FAST qPCR Master Mix 与 Helixyte Green 确保 PCR 特异性和灵敏度对所有样品类型，如基因组、质粒、病毒和 cDNA 模板。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*。 

 

适用仪器

qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无ROX* 解冻 TAQuest™ FAST qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D’Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7

Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6

Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine: 1234-8

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* 货号17286-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

货号 17286 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17286

产品名称：TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液，针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分，包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP，但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型（如基因组、质粒、病毒和 cDNA 模板）的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 

 

适用仪器

qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix 用于TaqMan探针*高ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 货号17289-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 货号17289 货号 17289 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17289

产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 

 

适用仪器

qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest  FAST qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*无ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 货号17291-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 货号17291 货号 17291 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17291

产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。

 

适用仪器

qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* 货号17292-AAT Bioquest荧光染料

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TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*

货号 17292 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17292

产品名称：TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液，针对 qPCR 和两步法 RT-qPCR 进行了优化，非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容，因此在 20 uL 反应体积中进行 40 个 PCR 循环，可在 50 分钟内提供结果。预混液提供所有基本成分，包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP，但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。

 

适用仪器

qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*高ROX* 12.5 µL 25 µL 1X
上游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物，10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70