美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616
- 产品型号： 560235
- 简单描述
- 美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616规格：0.1mg货号：560235库存：现货
美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616
美国BD公司Purified Mouse anti-TAZ抗体Clone M2-616
规格
0.1 mg
货号
560235
目录价
¥ 5,543.00
Brand
BD Pharmingen™
Alternative Name
WWTR1
Concentration
0.5 mg/ml
Isotype
Mouse BALB/c IgG2b, κ
Reactivity
Human (QC Testing) Mouse (Tested in Development)
Application
Western blot (Routinely Tested)
Bioimaging (Tested During Development)
Immunogen
Human TAZ Recombinant Protein
Storage Buffer
Aqueous buffered solution containing ≤0.09% sodium azide.
详细说明：
DESCRIPTION
Taz is a 49-kDa transcriptional co-activator with a PDZ-binding motif and is regulated by binding with 14-3-3 proteins. It plays a key role in differentiation of mesenchymal stem cells into either osteoblasts or adipocytes via interactions with key transcription factors Runx2 and PPAR γ . More recently, Taz was found to be a component of an E3 ubiquitin ligase involved in ubiquitin-dependent substrate proteolysis by mediating its interaction with the F-box protein β -Trcp. Therefore, Taz has dual functions of regulating protein degradation and transcription.
FORMAT
Format
Purified
SUGGESTED COMPANION PRODUCTS
| Cat No. | Description | Size | |
|---|---|---|---|
| 611449 | HeLa Cell Lysate RUO | 500 µg |
|
| 554656 | Stain Buffer (FBS) RUO | 500 mL |
|
| 554655 | Fixation Buffer RUO | 100 mL |
|
| 554723 | Perm/Wash Buffer RUO | 100 mL |
|
| 554002 | HRP Goat Anti-Mouse Ig RUO | 1 mL |
|
RESOURCES & TOOLS
- Download TDS
PREPARATION AND STORAGE
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
PRODUCT NOTICES
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences。。ｃｏｍ/pharmingen/protocols for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Triton is a trademark of the Dow Chemical Company.
- This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159
BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159
- 产品型号： 560842
- 简单描述
- BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159宿主物种：mouse反应物中：human规格：100T克隆号：B159应用：流逝现货供应中
BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159
Brand：BD Pharmingen
Alternative Name：NCAM1; NCAM-1; Neural cell adhesion molecule 1; NCAM; NKH1; MSK39
Application：Flow cytometry (Routinely Tested)
Immunogen：Human NK Cells
Storage Buffer：Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
BD PerCP-Cy5.5 Mouse Anti-Human CD56抗体Clone B159
DESCRIPTION
The B159 monoclonal antibody specifically binds to CD56. CD56 is a heavily glycosylated adhesion protein that is present on a subpopulation of peripheral blood large granular lymphocytes that demonstrate natural killer activity. CD56 is also expressed on a subset of T cells but is not expressed on myeloid cells, erythrocytes or B cells. This antigen is a pan-NK-cell marker. CD56 is virtually identical to an isoform of the neural cell adhesion molecule (NCAM), a structure mediating homotypic and heterotypic cell-cell interactions.
FORMAT
Format
PerCP-Cy™5.5
Excitation Source
Blue 488 nm
Excitation Max
482 nm
Emission Max
695 nm
PerCP-Cy™5.5 is a tandem conjugate that combines PerCP with a cyanine dye. PerCP-Cy5.5 is not subject to photobeaching like PerCP and can be used with stream-in-air flow cytometers. It has less Fc receptor–mediated nonspecific staining than PE-Cy5. Additionally, the PerCP-Cy5.5 tandem conjugate is not as susceptible to fixative or light instability compared to APC-Cy7 and PE-Cy7.
Other Formats →
- APC
- Alexa Fluor® 488
- Alexa Fluor® 647
- Alexa Fluor® 700
- BB515
- Biotin
- FITC
- NA/LE
- PE
- PE-CF594
- PE-Cy™5
- PE-Cy™7
- Purified
- V450
SUGGESTED COMPANION PRODUCTS
| Cat No. | Description | Size | |
|---|---|---|---|
| 550795 | PerCP-Cy™5.5 Mouse IgG1 κ Isotype Control RUO | 0.1 mg | |
| 555899 | Lysing Buffer RUO | 100 mL |
RESOURCES & TOOLS
- Spectrum Viewer
- Panel Designer
- Download TDS
PREPARATION AND STORAGE
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.
BD基质胶MATRIGEL MATRIX 5ML
BD基质胶MATRIGEL MATRIX 5ML
- 产品型号： 356234
- 简单描述
- BD基质胶MATRIGEL MATRIX 5ML产地：BD*规格：5ml大量现货促销中
BD基质胶MATRIGEL MATRIX 5ML
产品介绍：
BD Matrigel 基底膜/基质胶形成的三维培养基质，可促进上皮细胞、肝细胞、Sertoli细胞、黑色素瘤细胞、血管内皮细胞、甲状腺细胞及毛囊细胞等的贴壁与分化。同时，Matrigel还能影响乳腺上皮细胞的蛋白表达，支持外周神经的新生和牛输卵管上皮细胞的分化。
BD基质胶MATRIGEL MATRIX 5ML
使用指南：培养细胞时，Matrigel可无需稀释直接包被，包被厚度可为0.5 mm（薄层）或1.0 mm(厚层）。Matrigel也可以在无血清培养基中稀释后用于包被培养器皿表面，根据细胞类型和应用目的确定相应Matrigel浓度。
保存条件和效期：-20℃下冷冻保存，避免多次冻融。保质期详见产品说明。
纤维粘连蛋白（FN）
纤维粘连蛋白(FN)存在于间隙基质和血浆中，通过RGD序列介导细胞的粘附。FN的主要功能是在伤口愈合过程中促进细胞的迁移。有FN包被的细胞培养表面，对细胞的粘附，伸展和增殖有促进作用。FN也可以用作无血清培养基的添加物。
标准型
BD Matrigel是从富含胞外基质蛋白的EHS小鼠肿瘤中提取出基底膜基质，其主要成分有层粘连蛋白、Ⅳ型胶原、巢蛋白、硫酸肝素糖蛋白，还包含生长因子和基质金属蛋白酶等。在室温条件下，Matrigel聚合形成具有生物学活性的三维基质，模拟体内细胞基底膜的结构、组成、物理特性和功能，有利于体外细胞的培养和分化，可用于对细胞形态、生化功能、迁移、侵染和基因表达等的研究。
BD Matrigel基底膜/基质胶形成的三维培养基质，可促进上皮细胞、肝细胞、Sertoli细胞、黑色素瘤细胞、血管内皮细胞、甲状腺细胞及毛囊细胞等的贴壁与分化。同时，Matrigel还能影响乳腺上皮细胞的蛋白表达，支持外周神经的新生和牛输卵管上皮细胞的分化。
不同配方的BD Matrigel可以满足不同的实验要求。低生长因子（GFR）的Matrigel基底膜基质适用于对基质成分要求严格的实验，如细胞信号通路和细胞因子的研究等。不含酚红的Matrigel适用于荧光检测或显色反应等分析。高浓度的Matrigel适用于研究血管生成、肿瘤细胞迁移和体内肿瘤模型的建立等。
其他相关产品信息：
|
BD Matrigel基底膜基质 |
5 ml |
标准型 |
356234 |
|
|
10 ml |
|
354234 |
|
|
5×10 ml |
|
356235 |
|
|
10 ml |
高浓度(HC) |
354248 |
|
BD Matrigel基底膜基质，无酚红 |
10 ml |
标准型 |
356237 |
|
|
10 ml |
高浓度(HC) |
354262 |
|
BD Matrigel基底膜基质，低生长因子 |
5 ml |
标准型 |
356230 |
|
|
10 ml |
标准型 |
354230 |
|
|
10 ml |
高浓度(HC) |
354263 |
|
BD Matrigel基底膜基质，GFR，无酚红 TM |
10 ml |
标准型 |
356231 |
来源：EHS小鼠肿瘤
使用指南：培养细胞时， Matrigel 保存条件和效期：-20℃下冷冻保存，避免多次冻融。保质期详见产品说明。可无需稀释直接包被，包被厚度可为0.5 mm(薄层)或1.0 mm(厚层)。Matrigel也可以在无血清培养基中稀释后用于包被培养器皿表面，根据细胞类型和应用目的确定相应Matrigel浓度。
纤维粘连蛋白(FN)
纤维粘连蛋白(FN)存在于间隙基质和血浆中，通过RGD序列介导细胞的粘附。FN的主要功能是在伤口愈合过程中促进细胞的迁移。有FN包被的细胞培养表面，对细胞的粘附，伸展和增殖有促进作用。FN也可以用作无血清培养基的添加物。
|
产品描述 |
数量 |
货号 |
|
BD TM 纤维粘连蛋白，人 |
1 mg |
354008 |
|
|
5 mg |
356008 |
来源：人血浆
分子量：440 kD
使用指南：根据细胞类型不同，推荐工作浓度为1-5 μg/cm2生长面积或5 ug/ml培养添加剂。
保存条件和效期：冻干粉2-8℃下保存或溶解后-20℃下保存，避免多次冻融。保质期详见产品包装。
葡萄糖转运/血糖通道荧光探针2-NBDG CAS 186689-07-6-AAT Bioquest荧光染料
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
葡萄糖转运/血糖通道荧光探针2-NBDG CAS 186689-07-6
价格 2111
产品规格
产品货号
| Ex (nm) | 467 | Em (nm) | 538 |
| 分子量 | 342.26 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品基本信息
产品名称：葡萄糖转运/血糖通道荧光探针2-NBDG
CAS：186689-07-6
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：342.26
外观：红色固体
溶剂：DMSO
激发波长（nm）：485
发射波长（nm）：540
产品介绍
葡萄糖转运/血糖通道荧光探针2-NBDG是美国AAT Bioquest生产的用于检测葡萄糖/血糖的荧光探针。2-NBDG是荧光标记的脱氧葡萄糖类似物，主要用于直接监测活细胞和组织对葡萄糖的摄取。它还用作检测瘤形成的局部造影剂。2-NBDG可用于实时共聚焦，高分辨率或宽视场荧光显微镜以及流式细胞术。可以通过氩激光在488nm激发探针以产生对环境敏感的荧光。它的光稳定性低于罗丹明荧光探针。金畔生物是AAT Bioquest的中国代理商，为您提供优质的葡萄糖转运/血糖通道荧光探针2-NBDG。
点击查看光谱
参考文献
NT1014, a novel biguanide, inhibits ovarian cancer growth in vitro and in vivo
Authors: Lu Zhang, Jianjun Han, Amanda L Jackson, Leslie N Clark, Joshua Kilgore, Hui Guo, Nick Livingston, Kenneth Batchelor, Yajie Yin, Timothy P Gilliam
Journal: Journal of Hematology & Oncology (2016): 91
Glutamine promotes ovarian cancer cell proliferation through the mTOR/S6 pathway
Authors: Lingqin Yuan, Xiugui Sheng, Adam K Willson, Dario R Roque, Jessica E Stine, Hui Guo, Hannah M Jones, Chunxiao Zhou, Victoria L Bae-Jump
Journal: Endocrine-related cancer (2015): 577–591
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