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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class I >> Cytokeratins
Datasheet PDF
SDS
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – pan Cytokeratin antibody [PCK-26] (ab6401)(enlarge)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – pan Cytokeratin antibody [PCK-26] (ab6401)(enlarge)
Western blot – pan Cytokeratin antibody [PCK-26] (ab6401)(enlarge)
Applications
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Our Abpromise guarantee covers the use of ab6401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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WB
Four stars(5 Abreviews)WB: Use at an assay depe…Read more →
WB: Use at an assay dependent dilution.
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IHC-FoFr
IHC-FoFr: Use at an assa…Read more →
IHC-FoFr: Use at an assay dependent dilution.
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IHC-P
Five stars(3 Abreviews)IHC-P: Use at an assay d…Read more →
IHC-P: Use at an assay dependent dilution.
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IHC-Fr
Four stars(3 Abreviews)IHC-Fr: Use at an assay …Read more →
IHC-Fr: Use at an assay dependent dilution.
IF
IF: 1/300.
IF: 1/300.
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Dot Blot
Dot: Use at an assay dep…Read more →
Dot: Use at an assay dependent dilution.
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Flow Cyt
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Flow Cyt: Use at an assay dependent dilution. PubMed: 19855980Use 10µl for 5 x 107 cells.
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ICC/IF
Five stars(2 Abreviews)ICC/IF: 1/300.
ICC/IF: 1/300.
Target
Relevance
Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts.
Cellular localization
Cytoplasmic
Anti-pan Cytokeratin antibody [PCK-26] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Mouse liver tumor tissue sections by IHC-P (Paraformaldehyde fixed paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with maleate buffer blocking solution for 30 minutes and 22°C. Antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500) in maleate buffer blocking solution for 16 hours at 22°C. An undiluted biotin-conjugated Donkey polyclonal to mouse IgG was used as secondary antibody.
This image is courtesy of an Abreview submitted by Dr Asha Seth
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) – pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in mouse pancreatic ductal adenocarcinoma (left-hand panel) and mouse pancreatic neoplasia (right-hand panel) sections by immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; heat mediated antigen retrieval was performed. The sample was incubated with primary antibody (1/250) at 4°C for 8 hours. A Biotin-conjugated Goat polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
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Western blot – pan Cytokeratin antibody [PCK-26] (ab6401)
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
All lanes: pan Cytokeratin antibody (ab6401) at 1/1000 dilution + whole cell lysate of Primary mouse pancreatic cancer cell lines (35 µg)
Secondary: An HRP-conjugated Sheep anti-mouse IgG polyclonal (1/5000) developed using the ECL technique
Performed under non-reducing conditions.
Blocking Step: 10% Milk for 1 hour at room temperature
This image is courtesy of an Abreview submitted by Pawel Mazur
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Immunohistochemistry (Frozen sections) – pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Mouse pancreatic neoplasia tissue sections by Immunohistochemistry (IHC-Fr – frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/250 in PBS) for 8 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
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Immunocytochemistry/ Immunofluorescence – pan Cytokeratin antibody [PCK-26] (ab6401)
ab6401 staining pan Cytokeratin in Human Panc1 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone/methanol (1:1) and blocked with 1% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 2 hours. An Alexa Fluor®488-conjugated DGoat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI.
This image is courtesy of an anonymous Abreview
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References for Anti-pan Cytokeratin antibody [PCK-26] (ab6401)
This 25 mm Isopore hydrophilic membrane with 5.0 μm pore size is a polycarbonate, track-etched screen filter recommended for analyses of airborne contaminants & other particles using optical or electron microscopy.
为方便计算连接温度,请登陆 Ligasecalc.neb.com 使用 Thermostable Ligase Reaction Temperature Calculator在线工具。
概述
Taq 高保真 DNA 连接酶含热稳定 DNA 连接酶和专属试剂,具有无以伦比的保真性,能高保真且高效地连接 DNA 切刻。在优化的缓冲液体系中,该酶将与靶 DNA 互补的相邻 5´-磷酸基和 3´-羟基的切刻位点以磷酸二酯键进行连接,极大程度降低了错配率。改良的配方使得该产品可以更好的识别连接反应供体和受体,从而可以精确检测 SNPs 和其他等位基因变异。Taq 高保真 DNA 连接酶在 37–75℃ 温度范围内都有活性。
来源
纯化自重组的 E. coli 菌株,携带有克隆自嗜热菌的连接酶基因。
反应条件
1X Taq 高保真 DNA 连接酶反应缓冲液
[20 mM Tris-HCl,150 mM KCl,10 mM MgCl2,10mM DTT,1 mM NAD 和 0.1% Triton X-100(pH 8.5 @25℃)],25℃ 温育。
质保声明
Taq 高保真 DNA 连接酶经过严格的质控检测,确保该产品具有最高的活性和纯度。详情请登陆 www.neb.com 或 www.neb-china.com。
Individual Grid Screen Sodium Chloride screen reagents for screening, optimization and production
Features
185 ml volume
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-932-** ** = Refers to the reagent number in the kit. For example, reagent number 1 = HR2-932-A1
Individual reagents from the Grid Screen Sodium Chloride. 185 milliliters of screen solution, sterile filtered. ** = Refers to the reagent number in the kit. For example, Grid Screen Sodium Chloride reagent number A1 = Grid Screen Salt HT reagent G1 = HR2-932-A1.
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Grid Screen Sodium Chloride Individual Reagents
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CAT NO
HR2-932-A1
NAME
Grid Screen Sodium Chloride A1 (G1) 185ml
DESCRIPTION
0.1 M Citric acid pH 4.0, 1.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-A2
NAME
Grid Screen Sodium Chloride A2 (G2) 185ml
DESCRIPTION
0.1 M Citric acid pH 5.0, 1.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-A3
NAME
Grid Screen Sodium Chloride A3 (G3) 185ml
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 1.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-A4
NAME
Grid Screen Sodium Chloride A4 (G4) 185ml
DESCRIPTION
0.1 M HEPES pH 7.0, 1.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-A5
NAME
Grid Screen Sodium Chloride A5 (G5) 185ml
DESCRIPTION
0.1 M Tris pH 8.0, 1.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-A6
NAME
Grid Screen Sodium Chloride A6 (G6) 185ml
DESCRIPTION
0.1 M BICINE pH 9.0, 1.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-B1
NAME
Grid Screen Sodium Chloride B1 (G7) 185ml
DESCRIPTION
0.1 M Citric acid pH 4.0, 2.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-B2
NAME
Grid Screen Sodium Chloride B2 (G8) 185ml
DESCRIPTION
0.1 M Citric acid pH 5.0, 2.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-B3
NAME
Grid Screen Sodium Chloride B3 (G9) 185ml
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 2.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-B4
NAME
Grid Screen Sodium Chloride B4 (G10) 185ml
DESCRIPTION
0.1 M HEPES pH 7.0, 2.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-B5
NAME
Grid Screen Sodium Chloride B5 (G11) 185ml
DESCRIPTION
0.1 M Tris pH 8.0, 2.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-B6
NAME
Grid Screen Sodium Chloride B6 (G12) 185ml
DESCRIPTION
0.1 M BICINE pH 9.0, 2.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-C1
NAME
Grid Screen Sodium Chloride C1 (H1) 185ml
DESCRIPTION
0.1 M Citric acid pH 4.0, 3.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-C2
NAME
Grid Screen Sodium Chloride C2 (H2) 185ml
DESCRIPTION
0.1 M Citric acid pH 5.0, 3.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-C3
NAME
Grid Screen Sodium Chloride C3 (H3) 185ml
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 3.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-C4
NAME
Grid Screen Sodium Chloride C4 (H4) 185ml
DESCRIPTION
0.1 M HEPES pH 7.0, 3.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-C5
NAME
Grid Screen Sodium Chloride C5 (H5) 185ml
DESCRIPTION
0.1 M Tris pH 8.0, 3.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-C6
NAME
Grid Screen Sodium Chloride C6 (H6) 185ml
DESCRIPTION
0.1 M BICINE pH 9.0, 3.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-D1
NAME
Grid Screen Sodium Chloride D1 (H7) 185ml
DESCRIPTION
0.1 M Citric acid pH 4.0, 4.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-D2
NAME
Grid Screen Sodium Chloride D2 (H8) 185ml
DESCRIPTION
0.1 M Citric acid pH 5.0, 4.0 M Sodium chloride
PRICE
$171.00
cart quote
CAT NO
HR2-932-D3
NAME
Grid Screen Sodium Chloride D3 (H9) 185ml
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 4.0 M Sodium chloride
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).