Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
Small molecule dye penetrates solvent channels of macromolecular crystals, coloring the crystals blue
Salt crystals cannot absorb Izit and remain colorless
Differentiate protein and non-protein based phase separation
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
A crystal! Is it protein or is it salt?
Have you ever asked yourself this question? Sure you could mount the crystal in question and take a quick look at the diffraction pattern but perhaps you′re too lazy or you don′t have an x-ray facility. Well, there′s always the crush test. One simply takes a MicroTool™ or Crystal Probe™ and crushes the suspect crystal. A click or solid crunch is indicative of salt while a powder or silent destruction might indicate one just destroyed a perfectly good protein crystal. Izit is here to help. Simply place 1 µl of Izit in the sample drop and wait for an hour or so. Izit is a small molecule dye which will fill the solvent channels in protein crystals, coloring the crystals blue. With the appropriate dilution, Izit will in fact leave a clear drop with blue crystals, as illustrated in the picture above. Salt crystals do not possess these large solvent channels. Therefore, Izit cannot enter the crystal, leaving one with a clear crystal and a blue drop. Izit is especially nice for small microcrystals or questionable precipitate. The 0.5 ml vial of Izit is sufficient for more than thousands of crystallization drops.
Each Izit vial contains 0.5 ml of Izit dye. All solutions are formulated using ultra-pure water and are sterile filtered. Crystallization accessories are sold separately.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status
RUO
BD代理APC Mouse IgG2b κ Isotype Control Clone 27-35货号:555745
产品详情:
INTENDED USE
The mouse IgG2b, κ immunoglobulin isotype control monoclonal antibody 27-35 is specific for the hapten dansyl (5-[dimethylamino] naphthalene-1-sulfonyl). This hapten is not expressed on human cells or human cell lines. The 27-35 immunoglobulin was selected as an isotype control following testing which demonstrated low background staining on a variety of mouse and human tissues.
FORMAT
Format
APC
Excitation Source
Red 633 nm
Excitation Max
650 nm
Emission Max
660 nm
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximay 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
Other Formats →
APC-Cy™7
APC-H7
APC-R700
Alexa Fluor® 488
Alexa Fluor® 647
Alexa Fluor® 700
BB515
BUV395
BUV563
BUV737
BV421
BV510
BV650
BV711
BV786
Biotin
FITC
PE
PE-CF594
PE-Cy™5
RESOURCES & TOOLS
Download TDS
STORAGE AND HANDLING
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
PRODUCT NOTICES
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences。。com/pharmingen/protocols for technical protocols.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences。。com/colors.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Oakton Waterproof PD 650 Meter with probes with NIST-traceable Calibration
Top-of-the-line dual parameter meters offer features typically found on much more expensive models!
Simultaneously measure your choice of pH, mV, or ISE and dissolved oxygen
Log up to 500 data points with GLP compliance
Wireless IrDA communication for truly waterproof interfacing capabilities
These 650 meters feature user-selectable “calibration due” alarms, automatic or manual standards recognition, and single or multiple calibration points—providing flexibility in meeting your quality objectives. The large backlit LCD shows the measured value, measured units, temperature, time, battery life, and menu options. Infrared (IrDA) wireless technology lets you easily download data without any cables.
PD 650 meters measure pH and ISE with a 0.001 maximum resolution. The PD 650 measures oxygen saturation and concentration using a galvanic dissolved oxygen probe.
What’s Included:
data management software, probe holder, and batteries.
Meter with probes adds: a double-junction pH electrode and a dissolved oxygen probe with 25-ft (7.6-m) cable.
Meter kits further add: pH buffer solutions, electrolyte solution, protective rubber boot, and hard carrying case.
产品规格
产品类型
pH计
仪表风格
手提
外形尺寸(W)
3-1/4
尺寸(“H)
7-1/4
尺寸(“D)
2-1/4
范围(pH值)
-2.000 20.000
范围(mV的)
±2000
范围(ISE)
0.001至19,900 ppm时,磨牙,或毫克/升
范围(溶解氧)
0到600%或49.49 mg / L的
范围(温度°C)
-10至110
范围(温华氏度)
14至230
分辨率(pH值)
0.1/0.01/0.001
分辨率(温度)
0.1°C /°F
分辨率(MV)
0.1
分辨率(ISE)
2个或3个数字
分辨率(溶解氧)
0.1%,0.01毫克/升
准确度(PH)
±0.002
准确度(温度)
±0.9°F(±0.5°C)
精度(mV的)
±0.2
精度(ISE)
0.5%FS(单价); 1%FS(价)
精度(溶解氧)
±2%,±0.2毫克/升
温度补偿
自动或手动
显示
液晶显示
接口
RS-232
校准
6
防水
是
缓冲液识别
美国NIST,DIN
功率
四节AA电池(附带)或可选的通用AC适配器
数据记录(点)
500
品牌
OAKTON
型号
PD 650
Specifications
Product Type
pH Meters
Meter style
Portable
Dimensions (” W)
3-1/4
Dimensions (” H)
7-1/4
Dimensions (” D)
2-1/4
Range (pH)
-2.000 to 20.000
Range (mV)
±2000
Range (ISE)
0.001 to 19,900 ppm, molar, or mg/L
Range (Dissolved oxygen)
0 to 600% or 49.49 mg/L
Range (Temperature °C)
-10 to 110
Range (Temperature °F)
14 to 230
Resolution (pH)
0.1/0.01/0.001
Resolution (Temperature)
0.1°C/°F
Resolution (mV)
0.1
Resolution (ISE)
2 or 3 digits
Resolution (Dissolved oxygen)
0.1%, 0.01 mg/L
Accuracy (pH)
±0.002
Accuracy (Temperature)
±0.9°F (±0.5°C)
Accuracy (mV)
±0.2
Accuracy (ISE)
0.5% FS (monovalent); 1% FS (divalent)
Accuracy (Dissolved oxygen)
±2%, ±0.2 mg/L
Temperature compensation
Automatic or manual
Display
LCD
Interface
RS-232
Calibration
6
Waterproof
Yes
Buffer recognition
USA, NIST, DIN
Power
Four AA batteries (included) or optional universal AC adapter
Measure samples as small as 150 µL—without immersing in solution!
Two-point autocalibration
Automatic range selection
Calibration and slope adjustment
Use this pocket-size, waterproof meter anywhere. Built-in sensor lets you take measurements two ways: by simply immersing meter tip in sample or by placing a small amount of your sample directly on sample pad. Results are instantly displayed on the convenient 3-1/2-digit LCD. Two-point autocalibration combines with buffer recognition at pH 4.00 and 7.00 buffer values for simple, quick calibration. Order replacement sensor cartidges and replacement solution kits (sold separately).
What’s Included:
carrying case, batteries, and a solution kit containing calibrating solution and deionized water.