THERMO代理pierce High Capacity Endotoxin Removal Res
产品型号: 88270
简单描述
THERMO代理pierce High Capacity Endotoxin Removal ResinThermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour while maximiz
详细介绍
THERMO代理pierce High Capacity Endotoxin Removal Resin
现货供应,详细产品信息:
Features of High Capacity Endotoxin Removal Resin:
• High capacity—bind up to 2,000,000 EU/mL† to eliminate >99% of endotoxins • Durable—re-use resin up to 10 times with no loss in performance • Selective—recover ≥85% of your protein sample • High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL • Economical—large-volume discounts available
The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. Endotoxin levels in protein samples are reduced by ≥99% in as little as 1 hour using our spin cup format, and protein recovery is ≥85%. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.
THERMO代理pierce High Capacity Endotoxin Removal Resin
Thermo Scientific RiboLock RNase Inhibitor inhibits the activity of RNases A (see Figure 1 in Supporting Data), B, and C by binding them in a noncompetitive mode at a 1:1 ratio. It does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H.
Highlights
Performs under a wide range of reaction conditions
Protects RNA from degradation at temperatures up to 55°C (see Figure 2 in Supporting Data)
Note
DTT provided in the Storage Buffer ensures stability during long term storage, but is not necessary for inhibitor activity.
Specifications
CategoryName
Concentration
40 U/µL
Definition of Activity Unit
One unit of the RiboLock RNase Inhibitor inhibits the activity of 5 ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/mL BSA, 100 ng/mL RNase A, 0.1 mg/mL [3H]-RNA, 50 mg/mL yeast RNA, 8 mM DTT.
Hazardous
No
Hazardous:
No
Inactivation
Inactivated by heating at 75°C for 10 min. Residual activity detectable after 10 min heating at 70°C.
Inhibition
Inhibitors: common denaturants (SDS, urea and all oxidizing reagents (p-chloromercuribenzoate, dissolved oxygen, ions in their higher oxidation states) strongly inhibit RiboLock RNase Inhibitor and release the RNase bound.
Molecular Weight
49.6 kDa monomer
Quality Control
The absence of ribonucleases, endo-, exodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
Functionally tested in RNA and cDNA synthesis.
Shelf Life:
Shipping Condition:
Shipping Information
Source
E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.
SpecificationName
SpecificationValue
Storage Buffer
The enzyme is supplied in 20 mM HEPES-NaOH (pH 7.5), 50 mM NaCl, 8 mM DTT, 0.03% v/v) ELUGENT Detergent and 50% (v/v) glycerol.
Storage Condition
-20 C
Storage Condition:
fermentasRNA酶抑制剂RiboLock RNase Inhibitor 火热促销中
促销产品明细:
DreamTaq Green PCR Master Mix (2×)
K1081
200rxns
500
300
K1082
1000rxns
2,000
1200
DreamTaq PCR Master Mix (2×)
K1071
200rxns
500
300
K1072
1000rxns
2,000
1200
PCR master Mix (2×)
K0171
200rxns
450
276
K0172
1000rxns
1,800
1080
Long PCR Enzyme Mix
K0181
100U
250
150
K0182
500U
1,000
600
DreamTaq DNA Polymerase
EP0701
200U
150
90
EP0702
500U
200
120
EP0703
5x500U
900
540
EP0704
20x500U
3,200
1920
DreamTaq Green DNA Polymerase
EP0711
200U
150
90
EP0712
500U
200
120
EP0713
5x500U
1,080
648
EP0714
20x500U
3,200
1920
TurboFect in vitro Transfection Reagent
R0531
1ml
2,000
1300
TurboFect siRNA Transfection Reagent
R1401
0.5ml
1,600
1040
TurboFect Protein Transfection Reagent
R1411
125ul
2,140
1391
TurboFect in vivo Transfection Reagent
R0541
120ul
3,000
1950
“赠送送到Hi”
品名
货号
包装
目录价¥
赠送说明
RevertAid First Strand cDNA Synthesis Kit
K1622
100rxns
1,600
买2个,赠1个K1081(DreamTaq Green PCR Master Mix),价值¥500
Maxima First Strand cDNA Synthesis Kit
K1641
50rxns
2,500
同时购买,赠2个K1081(DreamTaq Green PCR Master Mix),价值¥1000
GeneJET RNA Purification Kit
K0731
50preps
1,900
Maxima First Strand cDNA Synthesis Kit
K1642
200rxns
7,500
同时购买,赠2个K1082(DreamTaq Green PCR Master Mix),价值¥4000
GeneJET RNA Purification Kit
K0732
250preps
7,600
RevertAid Premium First Strand cDNA Synthesis Kit
K1651
50rxns
970
同时购买,赠2个K1081(DreamTaq Green PCR Master Mix),价值¥1000
GeneJET RNA Purification Kit
K0731
50preps
1,900
RevertAid Premium First Strand cDNA Synthesis Kit
K1652
200rxns
3,880
同时购买,赠2个K1082(DreamTaq Green PCR Master Mix),价值¥4000
Replacement Information 05-321 is a recommended replacement for MAB3090
millipore Phosphotyrosine Antibody, clone 4G10抗体
重要规格表
Species Reactivity
Key Applications
Host
Format
Antibody Type
A
ICC, WB, IP, IHC
M
Purified
Monoclonal Antibody
Description
Catalogue Number
05-321
Replaces
MAB3090
Brand Family
Upstate
Trade Name
Upstate
Description
Anti-Phosphotyrosine Antibody, clone 4G10?
Background Information
Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Product Information
Format
Purified
Control
Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates. Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediay, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
Presentation
0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4. Liquid at 2-8°C. IgG2bκ mouse monocolonal antibody produced in vitro by mouse-mouse hybridoma 4G10? (FOX-NY [NS-1 derivative] myeloma x spleen cells).
Applications
Application
Anti-Phosphotyrosine Antibody, clone 4G10 detects tyrosine phosphorylated proteins in all species. This unique monoclonal antibody is validated for use in IC, IH, IP, WB and is backed by hundreds of publications
Key Applications
Immunocytochemistry
Western Blotting
Immunoprecipitation
Immunohistochemistry
Application Notes
Immunoprecipitation: 2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.
Biological Information
Immunogen
Phosphotyramine-KLH
Clone
4G10?
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
This antibody recognizes tyrosine-phosphorylated proteins from all species.
Isotype
IgG2bκ
Species Reactivity
All
Antibody Type
Monoclonal Antibody
Modifications
Phosphorylation
Purification Method
Protein G-Sepharose? Chromatography
Molecular Weight
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Product Usage Statements
Quality Assurance
Routinely evaluated on EGF-treated human A431 carcinoma cells.
Western Blot Analysis: 0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; , Druker, B. J., 1989; Kanakura, Y., 1991).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
NOTE: DO NOT FREEZE. For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8oC. Save the supernatant for application.
NEBuilder 高保真 DNA 组装预混液可以高效、准确的组装 DNA 片段。不管片段的长度和末端匹配性如何,该方法均可以多片段无缝组装。还可以组装单链寡核苷酸或含 15-80 bp 重叠区的不同片段长度的 DNA 片段。主要用于合成生物学和多片段的一步克隆,该产品操作简单、灵活,以预混液形式提供。在同一反应缓冲液中有几种不同的酶活性:
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最终形成双链闭合的 DNA 分子, 可以用于 PCR、RCA 或其它的分子生物学应用,以及直接转化到大肠杆菌。
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