ReadiLink BSA偶联试剂盒

	货号	5501	存储条件	Multiple
	规格	2 Reactions	价格	2604
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

ReadiLink BSA偶联试剂盒 是美国AAT Bioquest生产的牛血清蛋白偶联试剂盒，牛血清白蛋白（BSA）是一种血清白蛋白，具有许多生物化学应用，包括ELISA（酶联免疫吸附测定），免疫印迹和免疫组织化学。像大多数丰富的血浆蛋白一样，它非常稳定和可溶。此外，67kDa蛋白质足够大且复杂至完全免疫原性。它含有每个分子的多个位点，用于使用胺反应性或羧基反应性交联剂有效缀合肽和其他抗原。因此，BSA是一种流行的载体蛋白，用于缀合半抗原和其他弱抗原，使其对抗体产生具有更高的免疫原性。这种ReadiLink™BSA共轭试剂盒主要用于简单制备用于免疫和抗体生产的半抗原载体结合物。 ReadiLink™BSA缀合试剂盒使用羧基反应性碳二亚胺作为交联剂，将半抗原与载体蛋白一步结合。所得的缀合物用于引发针对半抗原的免疫应答和抗体产生。羧基反应性碳二亚胺与肽和蛋白质上暴露的羧基和氨基反应形成稳定的键。这些试剂盒含有BSA，配制成与羧基反应性碳二亚胺反应相容的缓冲液和脱盐旋转柱，通过简单的离心步骤提供出色的蛋白质回收。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiLink BSA偶联试剂盒 。 

产品说明书

样品实验方案

简要概述

1.准备蛋白质溶液
2.准备半抗原解决方案
3.将蛋白质与半抗原混合到EDC中
4.在室温下孵育反应2小时
5.通过脱盐来纯化缀合物

操作步骤

1.准备BSA-Hepten共轭：

1.1将200μLDdH2 O加入到BSA（组分A）的小瓶中以制备10mg / mL溶液。

1.2在450μL缀合缓冲液（组分B）中溶解最多2mg半抗原。注意：一些半抗原可能具有有限的溶解度，使用DMSO（在最终的缀合溶液中<30％）首先溶解它。较高浓度的DMSO可能使载体蛋白不可逆地变性。

1.3将450μL半抗原溶液加入200μLBSA溶液中，得到Hapten-BSA混合溶液。

1.4将Hapten-BSA溶液加入一小瓶EDC（10mg）（组分C）中，通过温和混合溶解。在室温下孵育2小时。

1.5通过脱盐纯化缀合物以除去未反应的交联剂和蛋白质防腐剂（例如叠氮化钠）。

2.纯化BSA-Hepten结合物：

2.1使用前将1瓶纯化缓冲液（组分D）解冻至室温。未使用的缓冲液可以在4°C下储存1周。

2.2拧下脱盐柱（组件E）的底部盖子并松开盖子。将色谱柱放入收集管中。

2.3将柱以1,000g离心2分钟以除去储存溶液。

2.4取下盖子，慢慢地向柱子中加入1 mL纯化缓冲液。以1,000g离心2分钟，取出缓冲液。再重复此步骤3次，从收集管中丢弃缓冲液。

2.5将色谱柱放入新的收集管中，轻轻地将样品放入紧凑树脂床的中心。

2.6将柱以1,000g离心2分钟以收集样品。

2.7现在可以将Hapten-BSA缀合物用于免疫。如果要将Hapten-BSA缀合物储存超过几天，则对缀合物进行无菌过滤，并在4°C或-20°C下储存。注意：如果在一周内使用缀合物，可以使用PBS进行纯化。如果要冷冻缀合物，使用纯化缓冲盐（组分D）进行纯化。如果在缀合中使用DMSO，则制备具有相同百分比的用于缀合的DMSO的纯化缓冲盐。这将使脱盐期间塔中的沉淀最小化。如果在缀合过程中形成沉淀，则将沉淀的材料离心，收集上清液并保存沉淀物。净化上清液。将沉淀物与纯化的缀合物合并。

数据分析

参考文献

Optimization of the preparation process of vinblastine sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM)
Authors: Zu Y, Zhang Y, Zhao X, Zhang Q, Liu Y, Jiang R.
Journal: Int J Nanomedicine (2009): 321

Characterization of bovine serum albumin glycated with glucose, galactose and lactose
Authors: Ledesma-Osuna AI, Ramos-Clamont G, Vazquez-Moreno L.
Journal: Acta Biochim Pol (2008): 491

Improvement of functional properties of bovine serum albumin through phosphorylation by dry-heating in the presence of pyrophosphate
Authors: Enomoto H, Li CP, Morizane K, Ibrahim HR, Sugimoto Y, Ohki S, Ohtomo H, Aoki T.
Journal: J Food Sci (2008): C84

Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio
Authors: Zheng C, Bi J, Ma G, Su Z.
Journal: Artif Cells Blood Substit Immobil Biotechnol (2007): 568

Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions
Authors: Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD.
Journal: Anal Bioanal Chem (2007): 969

Effect of lyophilization on the structure and phase changes of PEGylated-bovine serum albumin
Authors: Tattini V, Jr., Parra DF, Polakiewicz B, Pitombo RN.
Journal: Int J Pharm (2005): 124

[Preparation of bovine serum albumin nanoparticles surface-modified with glycyrrhizin]
Authors: Mao SJ, Hou SX, Zhang LK, Jin H, Bi YQ, Jiang B.
Journal: Yao Xue Xue Bao (2003): 787

[Products of spontaneous conjugation of aflatoxins with bovine serum albumin: immunochemical properties]
Authors: Burkin AA, Kononenko GP, Soboleva NA.
Journal: Prikl Biokhim Mikrobiol (2003): 228

Generation of polyclonal antibody against mu-conotoxin GIIIA using an immunogen of [Cys(5)]mu-conotoxin GIIIA site-specifically conjugated with bovine serum albumin
Authors: Nakamura M, Oba Y, Mori T, Sato K, Ishida Y, Matsuda T, Nakamura H.
Journal: Biochem Biophys Res Commun (2002): 1037

Mice vaccinated with the O-antigen of Francisella tularensis LVS lipopolysaccharide conjugated to bovine serum albumin develop varying degrees of protective immunity against systemic or aerosol challenge with virulent type A and type B strains of the pathogen
Authors: Conlan JW, Shen H, Webb A, Perry MB.
Journal: Vaccine (2002): 3465

相关产品

氯霉素-BSA缀合物

	货号	50001	存储条件	在零下15度以下保存
	规格	1 mg	价格	6564
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：50001

产品名称：氯霉素-BSA缀合物

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品介绍

氯霉素-BSA缀合物用于开发通常在ELISA分析中使用的抗氯霉素抗体。 氯霉素-BSA共轭物在每个BSA上具有多个氯霉素分子，以最大化其免疫原性。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的氯霉素-BSA缀合物。 

参考文献

A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins
Authors: Gehring A, He X, Fratamico P, Lee J, Bagi L, Brewster J, Paoli G, He Y, Xie Y, Skinner C, Barnett C, Harris D.
Journal: Toxins (Basel) (2014): 1855

Development of antigen capture ELISA for the quantification of EIAV p26 protein
Authors: Hu Z, Chang H, Ge M, Lin Y, Wang X, Guo W.
Journal: Appl Microbiol Biotechnol (2014): 9073

Development of atom transfer radical polymer-modified gold nanoparticle-based enzyme-linked immunosorbent assay (ELISA)
Authors: Chen F, Hou S, Li Q, Fan H, Fan R, Xu Z, Zhala G, Mai X, Chen X, Liu Y.
Journal: Anal Chem (2014): 10021

Development of monoclonal antibody-based sandwich ELISA for detection of dextran
Authors: Wang SY, Li Z, Wang XJ, Lv S, Yang Y, Zeng LQ, Luo FH, Yan JH, Liang DF.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 334

Effect of different combinations of antibodies and enzyme labels on ELISA of progesterone
Authors: Kumari GL, P and ey PK, Nathsharma SS, Sharma SK, Kochhar G.
Journal: J Immunoassay Immunochem (2014): 157

Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices
Authors: Ramach and ran S, Fu E, Lutz B, Yager P.
Journal: Analyst (2014): 1456

Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels
Authors: Qu Z, Xu H, Xu P, Chen K, Mu R, Fu J, Gu H.
Journal: Anal Chem (2014): 9367

3-(10′-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein
Authors: Sakharov IY, Demiyanova AS, Gribas AV, Uskova NA, Efremov EE, Vdovenko MM.
Journal: Talanta (2013): 414

Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria
Authors: Samara P, Kalbacher H, Ioannou K, Radu DL, Livaniou E, Promponas VJ, Voelter W, Tsitsilonis O.
Journal: J Immunol Methods (2013): 54

ELISA for determination of total coagulation factor XII concentration in human plasma
Authors: Madsen DE, Sidelmann JJ, Overgaard K, Koch C, Gram JB.
Journal: J Immunol Methods (2013): 32

氧氟沙星-BSA缀合物

	货号	50020	存储条件	在零下15度以下保存
	规格	1 mg	价格	6564
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：50020

产品名称：氧氟沙星-BSA缀合物

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品介绍

氧氟沙星-BSA缀合物用于开发抗氧氟沙星抗体，通常在ELISA分析中使用。 氧氟沙星-BSA缀合物在每个BSA上具有多个氧氟沙星分子，以最大化其免疫原性。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的氧氟沙星-BSA缀合物。

参考文献

A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins
Authors: Gehring A, He X, Fratamico P, Lee J, Bagi L, Brewster J, Paoli G, He Y, Xie Y, Skinner C, Barnett C, Harris D.
Journal: Toxins (Basel) (2014): 1855

Development of antigen capture ELISA for the quantification of EIAV p26 protein
Authors: Hu Z, Chang H, Ge M, Lin Y, Wang X, Guo W.
Journal: Appl Microbiol Biotechnol (2014): 9073

Development of atom transfer radical polymer-modified gold nanoparticle-based enzyme-linked immunosorbent assay (ELISA)
Authors: Chen F, Hou S, Li Q, Fan H, Fan R, Xu Z, Zhala G, Mai X, Chen X, Liu Y.
Journal: Anal Chem (2014): 10021

Development of monoclonal antibody-based sandwich ELISA for detection of dextran
Authors: Wang SY, Li Z, Wang XJ, Lv S, Yang Y, Zeng LQ, Luo FH, Yan JH, Liang DF.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 334

Effect of different combinations of antibodies and enzyme labels on ELISA of progesterone
Authors: Kumari GL, P and ey PK, Nathsharma SS, Sharma SK, Kochhar G.
Journal: J Immunoassay Immunochem (2014): 157

Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices
Authors: Ramach and ran S, Fu E, Lutz B, Yager P.
Journal: Analyst (2014): 1456

Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels
Authors: Qu Z, Xu H, Xu P, Chen K, Mu R, Fu J, Gu H.
Journal: Anal Chem (2014): 9367

3-(10′-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein
Authors: Sakharov IY, Demiyanova AS, Gribas AV, Uskova NA, Efremov EE, Vdovenko MM.
Journal: Talanta (2013): 414

Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria
Authors: Samara P, Kalbacher H, Ioannou K, Radu DL, Livaniou E, Promponas VJ, Voelter W, Tsitsilonis O.
Journal: J Immunol Methods (2013): 54

ELISA for determination of total coagulation factor XII concentration in human plasma
Authors: Madsen DE, Sidelmann JJ, Overgaard K, Koch C, Gram JB.
Journal: J Immunol Methods (2013): 32

雌二醇-BSA缀合物

	货号	50552	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	17400
	Ex (nm)		Em (nm)
	分子量	N/A	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：50552

产品名称：雌二醇-BSA缀合物

规格：5mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

外观：固体

溶剂：水

产品介绍

雌二醇是雌激素类固醇激素和主要的女性性激素。 它也被称为17β-雌二醇。 雌二醇参与发情和月经女性生殖周期的调节。 它负责女性次生性特征的发展，对女性生殖组织和妊娠的发展和维持很重要。 它还在许多其他组织（包括骨骼，脂肪，皮肤，肝脏和大脑）中具有重要作用。 尽管男性的雌二醇水平比女性低得多，但雌二醇在男性中也起着重要的作用。 雌二醇测试可测量血液中激素雌二醇的含量。 也称为E2测试。 雌二醇实验具有许多临床上重要的应用。雌二醇-BSA缀合物是开发雌二醇缀合物和检测方法的一个方便的基础。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的雌二醇-BSA缀合物。 

参考文献

Luminescence-Sensing Tb-MOF Nanozyme for the Detection and Degradation of Estrogen Endocrine Disruptors.
Authors: Wang, Li and Chen, Yang
Journal: ACS applied materials & interfaces (2020): 8351-8358

Interventions for emergency contraception.
Authors: Shen, Jie and Che, Yan and Showell, Emily and Chen, Ke and Cheng, Linan
Journal: The Cochrane database of systematic reviews (2019): CD001324

Reusable chemiluminescent fiber optic aptasensor for the determination of 17β-estradiol in water samples.
Authors: Yang, Rong and Liu, Jiayao and Song, Dan and Zhu, Anna and Xu, Wenjuan and Wang, Hongliang and Long, Feng
Journal: Mikrochimica acta (2019): 726

Influence of natural organic matter on horseradish peroxidase-mediated removal of 17α-ethinylestradiol: Role of molecular weight.
Authors: Yang, Yun and Li, Jianhua and Shi, Huanhuan and Zhai, Li and Wang, Xing and Gao, Shixiang
Journal: Journal of hazardous materials (2018): 9-16

A novel enzyme-linked immunosorbent assay based on anti-lipovitellin monoclonal antibodies for quantification of zebrafish (Danio rerio) vitellogenin.
Authors: Wang, Jun and Wang, Wei and Tian, Hua and Zhang, Xiaona and Ru, Shaoguo
Journal: Ecotoxicology and environmental safety (2017): 78-83

Chemiluminescence immunoassays for estradiol and ethinylestradiol based on new biotinylated estrogen derivatives.
Authors: Kanso, Hussein and Inguimbert, Nicolas and Istamboulie, Georges and Barthelmebs, Lise and Calas-Blanchard, Carole and Noguer, Thierry
Journal: Analytical biochemistry (2017): 63-68

Degradation of organic pollutants mediated by extracellular peroxidase in simulated sunlit humic waters: A case study with 17β-estradiol.
Authors: Li, Jianhua and Zhang, Ya and Huang, Qingguo and Shi, Huanhuan and Yang, Yun and Gao, Shixiang and Mao, Liang and Yang, Xi
Journal: Journal of hazardous materials (2017): 123-131

Interventions for emergency contraception.
Authors: Shen, Jie and Che, Yan and Showell, Emily and Chen, Ke and Cheng, Linan
Journal: The Cochrane database of systematic reviews (2017): CD001324

Transformation of 17α-ethinylestradiol by simultaneous photo-enzymatic process in Humic water.
Authors: Yang, Yun and Li, Jianhua and Lu, Kun and Shi, Huanhuan and Gao, Shixiang
Journal: Chemosphere (2017): 432-438

Effect of estrogen on vagal afferent projections to the brainstem in the female.
Authors: Ciriello, John and Caverson, Monica M
Journal: Brain research (2016): 21-42

雌二醇-BSA偶联物

	货号	50551	存储条件
	规格	1 mg	价格	4740
	Ex (nm)		Em (nm)
	分子量	N/A	溶剂
	产品详细介绍

简要概述

产品基本信息

货号：50551

产品名称：雌二醇-BSA偶联物

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：N/A

外观：固体

溶剂：水

产品介绍

雌二醇是一种雌激素类固醇激素和主要的女性性激素。它也被称为 17 β-雌二醇。雌二醇参与调节发情和月经女性生殖周期。它负责女性第二性征的发育，对女性生殖组织的发育和维持以及妊娠具有重要意义。它对许多其他组织也有重要影响，包括骨骼、脂肪、皮肤、肝脏和大脑。尽管与女性相比，男性的雌二醇水平要低得多，但雌二醇在男性中也有重要作用。雌二醇测试测量血液中激素雌二醇的含量。它也称为 E2 测试。雌二醇测试具有许多临床上重要的应用。 Estradiol-BSA 偶联物是用于开发雌二醇抗体和检测的便捷构建模块。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的雌二醇-BSA偶联物。 

参考文献