PageTell 预染10至250 kDa蛋白质 Ladder 货号60081-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

PageTell 预染10至250 kDa蛋白质 Ladder

货号	60081	存储条件	在零下15度以下保存, 避免光照
规格	4X 250 ul	价格	3612
Ex (nm)		Em (nm)
分子量		溶剂	Water
产品详细介绍

简要概述

PageTell 预染10至250 kDa的蛋白质Ladder是一种即用型三色蛋白质标准品，在Tris-甘氨酸缓冲液中具有13种预染的蛋白质，其分子量范围从10至250 kDa。在SDS-PAGE（Tris-甘氨酸缓冲液）上分离时，除了两个参考带（分别在25 kDa和75 kDa处的一个绿色带和一个红色带）外，蛋白质与一个蓝色发色团共价偶联。即用型可帮助用户直接使用它，而无需在使用前加热，减少或添加样品缓冲液。它用于检测SDS-聚丙烯酰胺凝胶电泳过程中的蛋白质分离，验证膜（硝酸纤维素，PVDF或尼龙）上的Western转移效率以及蛋白质的大小。

参考文献

A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV).
Authors: Dai, Yifan and Molazemhosseini, Alireza and Liu, Chung Chiun
Journal: Biosensors (2017)

Identification of pili on the surface of Finegoldia magna–a gram-positive anaerobic cocci.
Authors: Murphy, Elizabeth C and Janulczyk, Robert and Karlsson, Christofer and Mörgelin, Matthias and Frick, Inga-Maria
Journal: Anaerobe (2014): 40-9

A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins.
Authors: Versura, Piera and Bavelloni, Alberto and Blalock, William and Fresina, Michela and Campos, Emilio C
Journal: Molecular vision (2012): 2526-37

A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis.
Authors: Kao, Chien-Han and Cheng, Chiu-Min and Chuang, Kuo-Hsiang and Chuang, Chih-Hung and Tzou, Shey-Cherng and Cheng, Ta-Chun and Hsieh, Yuan-Chin and Liao, Kuang-Wen and Wang, Yun-Ming and Chang, Long-Sen and Roffler, Steve R and Chen, Fang Ming and Cheng, Tian-Lu
Journal: Analytical biochemistry (2012): 1-3

[Characterization of Toxoplasma gondii proteins from various strains].
Authors: Yaman, Kerem and Akarsu, Gülay Aral and Güngör, Çiğdem and Ataoğlu, Haluk
Journal: Turkiye parazitolojii dergisi (2011): 133-6

Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography.
Authors: Hughes, Alex J and Herr, Amy E
Journal: Analytical chemistry (2010): 3803-11

Structural characterization of glycopeptides by N-terminal protein ladder sequencing.
Authors: Suzuki, Yusuke and Suzuki, Minoru and Nakahara, Yoshiaki and Ito, Yukishige and Ito, Emi and Goto, Naoko and Miseki, Kozo and Iida, Junko and Suzuki, Akemi
Journal: Analytical chemistry (2006): 2239-43

Climbing the protein ladder.
Authors: Dovichi, Norman J
Journal: Nature biotechnology (2004): 1242-3

Methods for on-chip protein analysis.
Authors: Caputo, Emilia and Moharram, Ramy and Martin, Brian M
Journal: Analytical biochemistry (2003): 116-24

A sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein ladder made of disulfide-bridged proteins.
Authors: Doucet, A and Beauregard, M
Journal: Analytical biochemistry (2001): 296-7

说明书

PageTell 预染10至250 kDa蛋白质 Ladder.pdf

mFluor Violet 550琥珀酰亚胺酯货号1153-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

mFluor Violet 550琥珀酰亚胺酯

货号	1153	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	2388
Ex (nm)	527	Em (nm)	550
分子量	1125.2	溶剂	DMSO
产品详细介绍

简要概述

产品基本信息

货号：1153

产品名称：mFluor Violet 550琥珀酰亚胺酯

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1125.2

外观：固体

溶剂：DMSO

产品介绍

mFluor 染料专为针对多色流式细胞仪的应用而开发。这些染料具有较大的斯托克斯位移，并且可以通过流式细胞仪的激光线（例如405 nm，488 nm和633 nm）很好地激发。 mFluor 紫色染料经过优化，可在405 nm的紫色激光下激发。 AAT Bioquest提供了由紫色激光激发的最大数量的荧光染料。 mFluor™Violet 550染料的荧光激发和发射最大值分别为〜405 nm和〜550 nm。这些光谱特性使其成为流式细胞仪应用程序的独特颜色。 mFluor Violet 550 SE相当稳定，并且对蛋白质氨基具有良好的反应性和选择性。 mFluor Violet 550 SE提供了一种方便的工具，可通过紫激光激发标记流式细胞仪应用中的单克隆，多克隆抗体或其他蛋白质（> 10 kDa）。

参考文献

High throughput pSTAT signaling profiling by fluorescent cell barcoding and computational analysis.
Authors: Tsai, Wanxia Li and Vian, Laura and Giudice, Valentina and Kieltyka, Jacqueline and Liu, Christine and Fonseca, Victoria and Gazaniga, Nathalia and Gao, Shouguo and Kajigaya, Sachiko and Young, Neal S and Biancotto, Angélique and Gadina, Massimo
Journal: Journal of immunological methods (2020): 112667

Lot-to-lot stability of antibody reagents for flow cytometry.
Authors: Böttcher, Sebastian and van der Velden, Vincent H J and Villamor, Neus and Ritgen, Matthias and Flores-Montero, Juan and Escobar, Hugo Murua and Kalina, Tomas and Brüggemann, Monika and Grigore, Georgiana and Martin-Ayuso, Marta and Lecrevisse, Quentin and Pedreira, Carlos E and van Dongen, Jacques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112294

First record of Doleschallia tongana (Lepidoptera: Nymphalidae) for Guam Island.
Authors: Manuel, Jake and Tennent, W John and Buden, Donald W and Moore, Aubrey
Journal: F1000Research (2018): 366

Optimization and standardization of fluorescent cell barcoding for multiplexed flow cytometric phenotyping.
Authors: Giudice, Valentina and Feng, Xingmin and Kajigaya, Sachiko and Young, Neal S and Biancotto, Angélique
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 694-703

Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110

The new violet laser dye, Krome Orange, allows an optimal polychromatic immunophenotyping based on CD45-KO gating.
Authors: Preijers, Frank W M B and Huys, Erik and Leenders, Marij and Nieto, Laura and Gautherot, Emmanuel and Moshaver, Bijan
Journal: Journal of immunological methods (2011): 42-51

Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Authors: Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31

Violet laser diodes in flow cytometry: an update.
Authors: Telford, William and Kapoor, Veena and Jackson, James and Burgess, Walter and Buller, Gayle and Hawley, Teresa and Hawley, Robert
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2006): 1153-60

说明书

mFluor Violet 550琥珀酰亚胺酯.pdf

mFluor Violet 505琥珀酰亚胺酯货号1154-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

mFluor Violet 505琥珀酰亚胺酯

货号	1154	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	2388
Ex (nm)	393	Em (nm)	504
分子量	786.93	溶剂	DMSO
产品详细介绍

简要概述

产品基本信息

货号：1154

产品名称：mFluor Violet 505琥珀酰亚胺酯

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：786.93

外观：固体

溶剂：DMSO

产品介绍

mFluor 染料专为针对多色流式细胞仪的应用而开发。这些染料具有较大的斯托克斯位移，并且可以通过流式细胞仪的激光线（例如405 nm，488 nm和633 nm）很好地激发。 mFluor 紫色染料经过优化，可在405 nm的紫激光下激发。 AAT Bioquest提供了由紫色激光激发的最大数量的荧光染料。 mFluor Violet 505染料的荧光激发和发射最大值分别为〜405 nm和〜505 nm。这些光谱特性使其成为流式细胞仪应用程序的独特颜色。 mFluor Violet 505 SE相当稳定，并且对蛋白质氨基具有良好的反应性和选择性。 mFluor Violet 505 SE提供了一种方便的工具，可通过紫激光激发标记流式细胞仪应用中的单克隆，多克隆抗体或其他蛋白质（> 10 kDa）。

参考文献

说明书

mFluor Violet 505琥珀酰亚胺酯.pdf

盐霉素-HRP缀合物货号50571-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

盐霉素-HRP缀合物

货号	50571	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	6060
Ex (nm)		Em (nm)
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：50571

产品名称：盐霉素-HRP缀合物

规格：1mg

储存条件：-15℃避光

保质期：12个月

产品介绍

盐霉素是一种由白色链霉菌产生的聚醚单羧酸。它可用于预防因猪瘟，中肠埃里氏菌，大头孢菌，布鲁内特氏菌，大肠埃希菌和大肠埃希菌引起的家禽球虫病。沙利霉素对某些革兰氏阳性细菌具有活性，而肠杆菌科则具有抗性。盐霉素对产气荚膜梭菌也有抑制作用，因此使用盐霉素可降低肉鸡坏死性肠炎的发生率。盐霉素被认为是标记残留物，皮肤/脂肪被认为是靶组织。与其他聚醚离子载体相比，盐霉素在鸡蛋中产生的残留水平相对较低。盐霉素-HRP 缀合物是通过ELISA定量盐霉素的基本试剂。

参考文献

Analysis of the Differences in the Expression of mRNAs and miRNAs Associated with Drug Resistance in Endometrial Cancer Cells Treated with Salinomycin.
Authors: Januszyk, Piotr and Januszyk, Krzysztof and Wierzbik-Strońska, Magdalena and Boroń, Dariusz and Grabarek, Beniamin
Journal: Current pharmaceutical biotechnology (2021): 541-548

Changes in the ceca microbiota of broilers vaccinated for coccidiosis or supplemented with salinomycin.
Authors: Orso, C and Stefanello, T B and Franceschi, C H and Mann, M B and Varela, A P M and Castro, I M S and Frazzon, J and Frazzon, A P G and Andretta, I and Ribeiro, A M L
Journal: Poultry science (2021): 100969

Combined delivery of salinomycin and docetaxel by dual-targeting gelatinase nanoparticles effectively inhibits cervical cancer cells and cancer stem cells.
Authors: Wang, Qin and Yen, Ying-Tzu and Xie, Chen and Liu, Fangcen and Liu, Qin and Wei, Jia and Yu, Lixia and Wang, Lifeng and Meng, Fanyan and Li, Rutian and Liu, Baorui
Journal: Drug delivery (2021): 510-519

Correction to: Salinomycin effectively eliminates cancer stem-like cells and obviates hepatic metastasis in uveal melanoma.
Authors: Zhou, Jingfeng and Liu, Shenglan and Wang, Yun and Dai, Wei and Zou, Hailin and Wang, Shubo and Zhang, Jing and Pan, Jingxuan
Journal: Molecular cancer (2021): 47

Effects of Scrophularia striata hydroalcoholic extract in comparison to salinomycin on growth performance, intestinal health and immunity in broiler chickens following a mixed-species Eimeria challenge.
Authors: Rostami, Farhad and Taherpour, Kamran and Ghasemi, Hossein Ali and Akbari Gharaei, Mohammad and Shirzadi, Hassan
Journal: Veterinary parasitology (2021): 109417

Effects of supplementation with narasin, salinomycin, or flavomycin on performance and ruminal fermentation characteristics of Bos indicus Nellore cattle fed with forage-based diets.
Authors: Limede, Arnaldo Cintra and Marques, Rodrigo S and Polizel, Daniel Montanher and Cappellozza, Bruno Ieda and Miszura, Alexandre Arantes and Barroso, José Paulo Roman and Storti Martins, André and Sardinha, Lairana Aline and Baggio, Marcelo and Pires, Alexandre Vaz
Journal: Journal of animal science (2021)

Ester derivatives of salinomycin efficiently eliminate breast cancer cells via ER-stress-induced apoptosis.
Authors: Kuran, Dominika and Flis, Sylwia and Antoszczak, Michał and Piskorek, Marlena and Huczyński, Adam
Journal: European journal of pharmacology (2021): 173824

Facile fabrication of a novel 3D rose like lanthanum doped zirconia decorated reduced graphene oxide nanosheets: An efficient electro-catalyst for electrochemical reduction of futuristic anti-cancer drug salinomycin during pharmacokinetic study [Biosens Bioelectron 150 (2020) 111849].
Authors: Alkahtani, Saad A and Mahmoud, Ashraf M and Mahnashi, Mater H and Ali, Ramadan and El-Wekil, Mohamed M
Journal: Biosensors & bioelectronics (2021): 113202

Green one-pot synthesis of nitrogen and sulfur co-doped carbon quantum dots as new fluorescent nanosensors for determination of salinomycin and maduramicin in food samples.
Authors: Magdy, Galal and Abdel Hakiem, Ahmed F and Belal, Fathalla and Abdel-Megied, Ahmed M
Journal: Food chemistry (2021): 128539

In Vitro Antiviral Activities of Salinomycin on Porcine Epidemic Diarrhea Virus.
Authors: Yuan, Chen and Huang, Xintong and Zhai, Ruiyu and Ma, Yichao and Xu, Anyuan and Zhang, Penghao and Yang, Qian
Journal: Viruses (2021)

说明书

盐霉素-HRP缀合物.pdf

盐霉素-BSA缀合物货号50572-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

盐霉素-BSA缀合物

货号	50572	存储条件	在零下15度以下保存, 避免光照
规格	5 mg	价格	11628
Ex (nm)		Em (nm)
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：50572

产品名称：盐霉素-BSA缀合物

规格：5mg

储存条件：-15℃避光

保质期：12个月

产品介绍

盐霉素是一种由白色链霉菌产生的聚醚单羧酸。它可用于预防因猪瘟，中肠埃里氏菌，大头孢菌，布鲁内特氏菌，大肠埃希菌和大肠埃希菌引起的家禽球虫病。沙利霉素对某些革兰氏阳性细菌具有活性，而肠杆菌科则具有抗性。盐霉素对产气荚膜梭菌也有抑制作用，因此使用盐霉素可降低肉鸡坏死性肠炎的发生率。盐霉素被认为是标记残留物，皮肤/脂肪被认为是靶组织。与其他聚醚离子载体相比，盐霉素在鸡蛋中产生的残留水平相对较低。盐霉素-BSA缀合物是通过ELISA定量盐霉素的基本试剂。

参考文献

Salinomycin Modulates the Expression of mRNAs and miRNAs Related to Stemness in Endometrial Cancer.
Authors: Talkowski, Karol and Kiełbasiński, Kamil and Peszek, Wojciech and Grabarek, Beniamin O and Boroń, Dariusz and Oplawski, Marcin
Journal: Current pharmaceutical biotechnology (2021): 317-326

Simultaneous immunodetection of ionophore antibiotics, salinomycin and narasin, in poultry products and milk.
Authors: Burkin, Maksim A and Galvidis, Inna A
Journal: Analytical methods : advancing methods and applications (2021): 1550-1558

Effect of Salinomycin on Expression Pattern of Genes Associated with Apoptosis in Endometrial Cancer Cell Line.
Authors: Kiełbasiński, Kamil and Peszek, Wojciech and Grabarek, Beniamin O and Boroń, Dariusz and Wierzbik-Strońska, Magdalena and Oplawski, Marcin
Journal: Current pharmaceutical biotechnology (2020): 1269-1277

Evaluation of Variances in VEGF-A-D and VEGFR-1-3 Expression in the Ishikawa Endometrial Cancer Cell Line Treated with Salinomycin and An-ti-Angiogenic/Lymphangiogenic Effect.
Authors: Kras, Piotr and Talkowski, Karol and Grabarek, Beniamin Oskar and Dziobek, Nina and Boroń, Dariusz and Oplawski, Marcin
Journal: Current pharmaceutical biotechnology (2020)

Combination of salinomycin and radiation effectively eliminates head and neck squamous cell carcinoma cells in vitro.
Authors: Gehrke, Thomas and Hackenberg, Stephan and Polat, Bülent and Wohlleben, Gisela and Hagen, Rudolf and Kleinsasser, Norbert and Scherzad, Agmal
Journal: Oncology reports (2018): 1991-1998

Salinomycin Abolished STAT3 and STAT1 Interactions and Reduced Telomerase Activity in Colorectal Cancer Cells.
Authors: Chung, Seyung S and Adekoya, Debbie and Enenmoh, Ikechukwu and Clarke, Orette and Wang, Piwen and Sarkyssian, Marianna and Wu, Yong and Vadgama, Jaydutt V
Journal: Anticancer research (2017): 445-453

Geno- and cytotoxicity of salinomycin in human nasal mucosa and peripheral blood lymphocytes.
Authors: Scherzad, Agmal and Hackenberg, Stephan and Schramm, Carolin and Froelich, Katrin and Ginzkey, Christian and Hagen, Rudolf and Kleinsasser, Norbert
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2015): 813-8

Phenethyl isothiocyanate upregulates death receptors 4 and 5 and inhibits proliferation in human cancer stem-like cells.
Authors: Wang, Dan and Upadhyaya, Bijaya and Liu, Yi and Knudsen, David and Dey, Moul
Journal: BMC cancer (2014): 591

Sample treatment platform using nanoparticles to determine salinomycin in flesh and meat.
Authors: Park, Jihyun and Lim, H B
Journal: Food chemistry (2014): 112-7

Biodegradation of polyether algal toxins–isolation of potential marine bacteria.
Authors: Shetty, Kateel G and Huntzicker, Jacqueline V and Rein, Kathleen S and Jayachandran, Krish
Journal: Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering (2010): 1850-7

说明书

盐霉素-BSA缀合物.pdf

盐霉素-KLH缀合物货号50573-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

盐霉素-KLH缀合物

货号	50573	存储条件	在零下15度以下保存, 避免光照
规格	5 mg	价格	11628
Ex (nm)		Em (nm)
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：50573

产品名称：盐霉素-KLH缀合物

规格：5mg

储存条件：-15℃避光

保质期：12个月

产品介绍

盐霉素是一种由白色链霉菌产生的聚醚单羧酸。它可用于预防因猪瘟，中肠埃里氏菌，大头孢菌，布鲁内特氏菌，大肠埃希菌和大肠埃希菌引起的家禽球虫病。沙利霉素对某些革兰氏阳性细菌具有活性，而肠杆菌科则具有抗性。盐霉素对产气荚膜梭菌也有抑制作用，因此使用盐霉素可降低肉鸡坏死性肠炎的发生率。盐霉素被认为是标记残留物，皮肤/脂肪被认为是靶组织。与其他聚醚离子载体相比，盐霉素在鸡蛋中产生的残留水平相对较低。盐霉素-KLH缀合物是通过ELISA定量盐霉素的基本试剂。

参考文献

Molecular characterization and analysis of the ATPase ASNA1 homolog gene of Eimeria tenella in a drug sensitive strain and drug resistant strains.
Authors: Yu, Yu and Dong, Hui and Zhao, Qiping and Zhu, Shunhai and Liang, Shanshan and Wang, Qingjie and Wang, Haixia and Yu, Shuilan and Huang, Bing and Han, Hongyu
Journal: International journal for parasitology. Drugs and drug resistance (2021): 115-125

Molecular characterization and functional analysis of Eimeria tenella citrate synthase.
Authors: Wang, Haixia and Zhao, Qiping and Zhu, Shunhai and Dong, Hui and Yu, Shuilan and Wang, Qingjie and Yu, Yu and Liang, Shanshan and Zhao, Huanzhi and Huang, Bing and Han, Hongyu
Journal: Parasitology research (2021): 1025-1035

Targeted Delivery of Combination Therapeutics Using Monoclonal Antibody 2C5-Modified Immunoliposomes for Cancer Therapy.
Authors: Narayanaswamy, Radhika and Torchilin, Vladimir P
Journal: Pharmaceutical research (2021): 429-450

Enhanced targeting of prostate cancer-initiating cells by salinomycin-encapsulated lipid-PLGA nanoparticles linked with CD44 antibodies.
Authors: Wei, Jun and Sun, Jin and Liu, Yu
Journal: Oncology letters (2019): 4024-4033

Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer.
Authors: Hori, Ami and Shimoda, Masafumi and Naoi, Yasuto and Kagara, Naofumi and Tanei, Tomonori and Miyake, Tomohiro and Shimazu, Kenzo and Kim, Seung Jin and Noguchi, Shinzaburo
Journal: Breast cancer research : BCR (2019): 88

Promoted delivery of salinomycin sodium to lung cancer cells by dual targeting PLGA hybrid nanoparticles.
Authors: Zhou, Jie and Sun, Jin and Chen, Huaiwen and Peng, Qing
Journal: International journal of oncology (2018): 1289-1300

The enhanced delivery of salinomycin to CD133+ ovarian cancer stem cells through CD133 antibody conjugation with poly(lactic-co-glycolic acid)-poly(ethylene glycol) nanoparticles.
Authors: Mi, Yi and Huang, Yuqin and Deng, Jie
Journal: Oncology letters (2018): 6611-6621

CD44 targeted chemotherapy for co-eradication of breast cancer stem cells and cancer cells using polymeric nanoparticles of salinomycin and paclitaxel.
Authors: Muntimadugu, Eameema and Kumar, Rajendra and Saladi, Shantikumar and Rafeeqi, Towseef Amin and Khan, Wahid
Journal: Colloids and surfaces. B, Biointerfaces (2016): 532-546

Combination of drug-conjugated SWCNT nanocarriers for efficient therapy of cancer stem cells in a breast cancer animal model.
Authors: Al Faraj, Achraf and Shaik, Asma Sultana and Ratemi, Elaref and Halwani, Rabih
Journal: Journal of controlled release : official journal of the Controlled Release Society (2016): 240-51

Laser-induced fluorescence reader with a turbidimetric system for sandwich-type immunoassay using nanoparticles.
Authors: Kim, Y H and Lim, H B
Journal: Analytica chimica acta (2015): 32-6

说明书

盐霉素-KLH缀合物.pdf

PE-iFluor 750 串联染料货号2704-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

PE-iFluor 750 串联染料

货号	2704	存储条件	在2-8度冷藏保存, 避免光照
规格	1 mg	价格	2388
Ex (nm)	566	Em (nm)	778
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：2704

产品名称：PE-iFluor 750 串联染料

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

产品物理化学光谱特性

外观：固体

溶剂：水

激发波长(nm)：566

发射波长(nm)：778

产品介绍

PE-iFluor 750串联染料是流式细胞术中使用的一种新颜色染料。其主要吸收峰位于 565 nm，发射峰位于 ~770 nm。它已通过光谱流式细胞仪进行了验证。 PE-iFluor 750 串联染料的染色指数比相应的 PE-Alexa Fluor 750 或 PE-Cy7 串联染料更高。 AAT Bioquest 为常规和光谱流式细胞术应用提供最多的颜色，包括 iFluor 、mFluor 有机染料及其各种串联染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的PE-iFluor 750 串联染料。

点击查看光谱

参考文献

Fluorescent energy transfer causing misleading signal in multicolor flow cytometry.
Authors: Khenine, Hana and Waeckel, Louis and Seghrouchni, Fouad and Berger, Anne-Emmanuelle and Lambert, Claude
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2021)

The ISCCA flow protocol for the monitoring of anti-CD20 therapies in autoimmune disorders.
Authors: Gatti, Arianna and Buccisano, Francesco and Scupoli, Maria T and Brando, Bruno
Journal: Cytometry. Part B, Clinical cytometry (2021): 194-205

Probing Surface Membrane Receptors Using Engineered Bacteriophage Bioconjugates.
Authors: Lomakin, Yakov A and Kaminskaya, Alena N and Stepanov, Alexey V and Shmidt, Anna A and Gabibov, Alexander G and Belogurov, Alexey A
Journal: Bioconjugate chemistry (2019): 1500-1506

A ten-color tube with dried antibody reagents for the screening of hematological malignancies.
Authors: Correia, R P and Rajab, A and Bento, L C and Alexandre, A M and Vaz, A C and Schimidell, D and Pedro, E C and Perin, F S and Nozawa, S T and Barroso, R S and Bacal, N S
Journal: International journal of laboratory hematology (2018): 136-143

Initial flow cytometric evaluation of the Clearllab lymphoid screen.
Authors: Hedley, B D and Cheng, G and Luider, J and Kern, W and Lozanski, G and Chin-Yee, I and Lowes, L E and Keeney, M and Careaga, D and Magari, R and Tejidor, L
Journal: Cytometry. Part B, Clinical cytometry (2018): 707-713

Reduced CD4 T Lymphocytes in Lymph Nodes of the Mouse Model of Autism Induced by Valproic Acid.
Authors: Baronio, Diego and Bauer-Negrini, Guilherme and Castro, Kamila and Della-Flora Nunes, Gustavo and Riesgo, Rudimar and Mendes-da-Cruz, Daniella Arêas and Savino, Wilson and Gottfried, Carmem and Bambini-Junior, Victorio
Journal: Neuroimmunomodulation (2018): 280-284

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080

Comparison of Flow-cytometric Antibody Secreting Cell Assay and Mabtech Immunoglobulin ELISpot Assay.
Authors: Lee, N and In, J W and Kim, H and Roh, E Y and Shin, S and Park, K U and Yang, J and Song, E Y
Journal: Transplantation proceedings (2017): 963-966

Semiconducting polymer dots with bright narrow-band emission at 800 nm for biological applications.
Authors: Chen, Dandan and Wu, I-Che and Liu, Zhihe and Tang, Ying and Chen, Haobin and Yu, Jiangbo and Wu, Changfeng and Chiu, Daniel T
Journal: Chemical science (2017): 3390-3398

[Changes of monocyte and monocyte-platelet aggregates in different subgroups of thrombotic events in patients with acute myocardial infarction during PCI].
Authors: Wang, Sheng and Sun, Cuifang and Liao, Wang and Wu, Zhongwei and Wang, Yudai and Huang, Xiuxian and Lu, Sijia and Dong, Xiaoli and Shuai, Fujie and Li, Bin
Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (2017): 959-965

说明书

PE-iFluor 750 串联染料.pdf

iFluor Ultra 594 琥珀酰亚胺酯货号71650-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor Ultra 594 琥珀酰亚胺酯

货号	71650	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	3612
Ex (nm)	586	Em (nm)	601
分子量	1436.72	溶剂	DMSO
产品详细介绍

简要概述

荧光染料缀合抗体是许多应用中鉴定蛋白质的一种工具，包括荧光细胞成像、流式细胞术、蛋白质印迹、免疫组织化学等。使用荧光标记抗体的优势包括更高的灵敏度、多路复用能力和易用性。 iFluor Ultra 系列是我们广受欢迎的 iFluor 染料的最新升级版，并针对用于荧光成像和流式细胞术应用的标记抗体进行了优化。用 iFluor Ultra 594 制备的抗体缀合物远远优于其他现有类似染料的缀合物，如 Alexa Fluor® 594。iFluor Ultra 594 缀合物在相同条件下比用 Alexa Fluor® 594 制备的缀合物更亮。此外，iFluor Ultra 594 的荧光不受 pH (4-10) 的影响。 iFluor Ultra 594 SE 染料相当稳定，并表现出与蛋白质氨基的良好反应性和选择性。 iFluor Ultra 594 的光谱特性和反应性类似于 Alexa Fluor® 594（Alexa Fluor® 是 ThermoFisher 的商标）。

产品说明书

实验方案

储备溶液配制

1. 蛋白质原液（溶液 A）
将 100 µL 反应缓冲液（例如，1 M 碳酸钠溶液或 1 M 磷酸盐缓冲液，pH ~ 9.0）与 900 µL 目标蛋白溶液（例如抗体，如果可能，蛋白质浓度 >2 mg/mL）混合，得到 1 mL 蛋白质标记原液。
注意：蛋白质溶液（溶液 A）的 pH 值应为 8.5 ± 0.5。如果蛋白质溶液的 pH 值低于 8.0，则使用 1 M 碳酸氢钠溶液或 1 M pH 9.0 磷酸盐缓冲液将 pH 值调整到 8.0-9.0 的范围内。
注意：蛋白质应溶于1X 磷酸盐缓冲盐水 (PBS)，pH 7.2-7.4。如果蛋白质溶解在 Tris 或甘氨酸缓冲液中，则必须用 1X PBS，pH 7.2-7.4 进行透析，以去除用于蛋白质沉淀的游离胺或铵盐（如硫酸铵和醋酸铵）。
注意：如果蛋白质浓度低于 2 mg/mL，缀合效率会显着降低。为获得最佳标记效率，建议最终蛋白质浓度范围为 2-10 mg/mL。

2. iFluor Ultra 594 SE 原液（溶液 B）
将无水 DMSO 添加到 iFluor Ultra 594 SE 小瓶中，制成 10 mM 储备溶液。通过移液或涡旋混合均匀。
注意：在开始缀合之前准备染料储备溶液（溶液 B），并及时使用。染料原液的长期储存可能会降低染料活性。溶液 B 在避光和防潮的情况下可以在冰箱中储存两周，避免冻融循环。

操作步骤

该标记方案是为山羊抗小鼠 IgG 与 iFluor Ultra 594 SE 的缀合而开发的。您可能需要进一步优化您的特定蛋白质。
注意：每种蛋白质需要不同的染料/蛋白质比例，这也取决于染料的特性。蛋白质的过度标记会对其缀合亲和力产生不利影响，而低染料/蛋白质比率的蛋白质缀合物会降低灵敏度。

1.进行缀合反应
1.1使用 10:1 的溶液 B（染料）/溶液 A（蛋白质）的摩尔比作为起点：将 5 μL 的染料储备溶液（溶液 B，假设染料储备溶液为 10 mM）在有效摇动下倒入蛋白质溶液（95 µL 溶液 A）小瓶中。假设蛋白质浓度为 10 mg/mL 且蛋白质的分子量为 ~200KD，则蛋白质的浓度为 ~0.05 mM。
注意：我们建议使用 10:1 摩尔比的溶液 B（染料）/溶液 A（蛋白质）。如果太低或太高，分别确定最佳染料/蛋白质比例为 5:1、15:1 和 20:1。
1.2在室温下继续旋转或摇动反应混合物 30-60 分钟。

2.纯化缀合物
以下方案是使用 Sephadex G-25 柱进行染料-蛋白质缀合物纯化的示例。
根据制造说明准备 Sephadex G-25 柱。
2.1将反应混合物（来自“1.进行缀合反应”）加载到 Sephadex G-25 列的顶部。
2.2样品刚好在顶部树脂表面下方运行时，立即添加 PBS (pH 7.2-7.4)。
2.3向所需样品中添加更多 PBS (pH 7.2-7.4) 以完成柱纯化。结合含有所需染料-蛋白质偶联物的组分。
注意：要立即使用，染料-蛋白质偶联物需要用染色缓冲液稀释，并分装多次使用。
注意：对于长期储存，染料-蛋白质缀合物溶液需要浓缩或冷冻干燥。

3.数据处理

3.1表征所需的染料-蛋白质缀合物
取代度 (DOS) 是表征染料标记蛋白质的最重要因素。较低 DOS 的蛋白质通常具有较弱的荧光强度，但较高 DOS 的蛋白质（例如 DOS > 6）也往往具有较低的荧光强度。大多数抗体的最佳 DOS 建议在 2 到 10 之间，具体取决于染料和蛋白质的特性。为了有效标记，应控制取代度，使 6-8 摩尔 iFluor Ultra 594 SE 对 1 摩尔抗体。以下步骤用于确定 iFluor Ultra 594 SE 标记蛋白质的 DOS。

3.2吸收检测
要检测染料-蛋白质缀合物的吸收光谱，建议将样品浓度保持在 1-10 µM 的范围内，具体取决于染料的消光系数。

3.3读取 280 nm 处的 OD（吸光度）和染料最大吸光度（对于 iFluor Ultra 594 染料，ƛmax = 588 nm）
对于大多数分光光度计，样品（来自色谱柱馏分）需要用去离子水稀释，以便 OD 值在 0.1 到 0.9 的范围内。 OD（吸光度）在 280 nm 是蛋白质的最大吸收，而 588 nm 是 iFluor Ultra 594 SE 的最大吸收。要获得准确的 DOS，请确保缀合物中不含非结合染料。

3.4计算DOS
您可以通过链接使用我们的工具计算DOS

参考文献

A fully integrated isotachophoresis with a programmable microfluidic platform.
Authors: Shebindu, Adam and Somaweera, Himali and Estlack, Zachary and Kim, Jungtae and Kim, Jungkyu
Journal: Talanta (2021): 122039

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).
Authors: Seo, Hyesu and Kim, Kyunghee and Park, Woong June
Journal: Biomolecules (2021)

Liquid Droplet Formation and Facile Cytosolic Translocation of IgG in the Presence of Attenuated Cationic Amphiphilic Lytic Peptides.
Authors: Iwata, Takahiro and Hirose, Hisaaki and Sakamoto, Kentarou and Hirai, Yusuke and Arafiles, Jan Vincent V and Akishiba, Misao and Imanishi, Miki and Futaki, Shiroh
Journal: Angewandte Chemie (International ed. in English) (2021)

MicroRNA-126 inhibits pathological retinal neovascularization via suppressing vascular endothelial growth factor expression in a rat model of retinopathy of prematurity.
Authors: Fan, Yuan-Yao and Liu, Chi-Hsien and Wu, An-Lun and Chen, Hung-Chi and Hsueh, Yi-Jen and Chen, Kuan-Jen and Lai, Chi-Chun and Huang, Chung-Ying and Wu, Wei-Chi
Journal: European journal of pharmacology (2021): 174035

Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.
Authors: Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R
Journal: Brain structure & function (2021)

Fluorescently-labeled fremanezumab is distributed to sensory and autonomic ganglia and the dura but not to the brain of rats with uncompromised blood brain barrier.
Authors: Noseda, Rodrigo and Schain, Aaron J and Melo-Carrillo, Agustin and Tien, Jason and Stratton, Jennifer and Mai, Fanny and Strassman, Andrew M and Burstein, Rami
Journal: Cephalalgia : an international journal of headache (2020): 229-240

Modeling iontophoretic drug delivery in a microfluidic device.
Authors: Moarefian, Maryam and Davalos, Rafael V and Tafti, Danesh K and Achenie, Luke E and Jones, Caroline N
Journal: Lab on a chip (2020): 3310-3321

Cell-based immunofluorescence assay for screening the neurogenesis potential of new drugs in adult hippocampal neural progenitor cells.
Authors: Zhang, Kun and Li, Bin and Li, Peifang and Yang, Xiaoli and Cui, Huixian and Liu, Xiaoyun
Journal: Acta neurobiologiae experimentalis (2019): 302-308

Super blinking and biocompatible nanoprobes based on dye doped BSA nanoparticles for super resolution imaging.
Authors: Zong, Shenfei and Pan, Fengmei and Zhang, Ruohu and Chen, Chen and Wang, Zhuyuan and Cui, Yiping
Journal: Nanotechnology (2019): 065701

[Sepsis impairs aggregation of nicotinic acetylcholine receptors on murine skeletal muscle cell membranes by inhibiting AKT/GSK3β phosphorylation].
Authors: Li, Tianmei and Liu, Li and Wang, Xiaobin
Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (2019): 1337-1343

说明书

iFluor Ultra 594 琥珀酰亚胺酯.pdf

iFluor Ultra 750 琥珀酰亚胺酯货号71680-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor Ultra 750 琥珀酰亚胺酯

货号	71680	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	3612
Ex (nm)	749	Em (nm)	773
分子量	1426.78	溶剂	DMSO
产品详细介绍

简要概述

荧光染料缀合抗体是许多应用中鉴定蛋白质的一种工具，包括荧光细胞成像、流式细胞术、蛋白质印迹、免疫组织化学等。使用荧光标记抗体的优势包括更高的灵敏度、多路复用能力和易用性。 iFluor Ultra 系列是我们广受欢迎的 iFluor 染料的最新升级版，并针对用于荧光成像和流式细胞术应用的标记抗体进行了优化。用 iFluor Ultra 750 制备的抗体缀合物远远优于其他现有类似染料的缀合物，如 Alexa Fluor® 750。iFluor Ultra 750 缀合物在相同条件下比用 Alexa Fluor® 750 制备的缀合物更亮。此外，iFluor Ultra 750 的荧光不受 pH (4-10) 的影响。 iFluor Ultra 750 SE 染料相当稳定，并表现出与蛋白质氨基的良好反应性和选择性。 iFluor Ultra 750 的光谱特性和反应性类似于 Alexa Fluor® 750（Alexa Fluor® 是 ThermoFisher 的商标）。

产品说明书

实验方案

储备溶液配制

2. iFluor Ultra 750 SE 原液（溶液 B）
将无水 DMSO 添加到 iFluor Ultra 750 SE 小瓶中，制成 10 mM 储备溶液。通过移液或涡旋混合均匀。
注意：在开始缀合之前准备染料储备溶液（溶液 B），并及时使用。染料原液的长期储存可能会降低染料活性。溶液 B 在避光和防潮的情况下可以在冰箱中储存两周，避免冻融循环。

操作步骤

该标记方案是为山羊抗小鼠 IgG 与 iFluor Ultra 750 SE 的缀合而开发的。您可能需要进一步优化您的特定蛋白质。
注意：每种蛋白质需要不同的染料/蛋白质比例，这也取决于染料的特性。蛋白质的过度标记会对其缀合亲和力产生不利影响，而低染料/蛋白质比率的蛋白质缀合物会降低灵敏度。

3.数据处理

3.1表征所需的染料-蛋白质缀合物
取代度 (DOS) 是表征染料标记蛋白质的最重要因素。较低 DOS 的蛋白质通常具有较弱的荧光强度，但较高 DOS 的蛋白质（例如 DOS > 6）也往往具有较低的荧光强度。大多数抗体的最佳 DOS 建议在 2 到 10 之间，具体取决于染料和蛋白质的特性。为了有效标记，应控制取代度，使 6-8 摩尔 iFluor Ultra 750 SE 对 1 摩尔抗体。以下步骤用于确定 iFluor Ultra 750 SE 标记蛋白质的 DOS。

3.2吸收检测
要检测染料-蛋白质缀合物的吸收光谱，建议将样品浓度保持在 1-10 µM 的范围内，具体取决于染料的消光系数。

3.3读取 280 nm 处的 OD（吸光度）和染料最大吸光度（对于 iFluor Ultra 750 染料，ƛmax = 588 nm）
对于大多数分光光度计，样品（来自色谱柱馏分）需要用去离子水稀释，以便 OD 值在 0.1 到 0.9 的范围内。 OD（吸光度）在 280 nm 是蛋白质的最大吸收，而 588 nm 是 iFluor Ultra 750 SE 的最大吸收。要获得准确的 DOS，请确保缀合物中不含非结合染料。

3.4计算DOS
您可以通过链接使用我们的工具计算DOS

参考文献

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).
Authors: Seo, Hyesu and Kim, Kyunghee and Park, Woong June
Journal: Biomolecules (2021)

说明书

iFluor Ultra 750 琥珀酰亚胺酯.pdf

iFluor Ultra 750 琥珀酰亚胺酯货号71681-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor Ultra 750 琥珀酰亚胺酯

货号	71681	存储条件	在零下15度以下保存, 避免光照
规格	100 ug	价格	1164
Ex (nm)	749	Em (nm)	773
分子量	1426.78	溶剂	DMSO
产品详细介绍

简要概述

荧光染料缀合抗体是许多应用中鉴定蛋白质的一种工具，包括荧光细胞成像、流式细胞术、蛋白质印迹、免疫组织化学等。使用荧光标记抗体的优势包括更高的灵敏度、多路复用能力和易用性。 iFluor Ultra 系列是我们广受欢迎的 iFluor 染料的最新升级版，并针对用于荧光成像和流式细胞术应用的标记抗体进行了优化。用 iFluor Ultra 750 制备的抗体缀合物远远优于其他现有类似染料的缀合物，如 Alexa Fluor® 750。iFluor Ultra 750 缀合物在相同条件下比用 Alexa Fluor® 750 制备的缀合物更亮。此外，iFluor Ultra 750 的荧光不受 pH (4-10) 的影响。 iFluor Ultra 750 SE 染料相当稳定，并表现出与蛋白质氨基的良好反应性和选择性。 iFluor Ultra 750 的光谱特性和反应性类似于 Alexa Fluor® 750（Alexa Fluor® 是 ThermoFisher 的商标）。

产品说明书

实验方案

储备溶液配制

操作步骤

3.数据处理

3.1表征所需的染料-蛋白质缀合物
取代度 (DOS) 是表征染料标记蛋白质的最重要因素。较低 DOS 的蛋白质通常具有较弱的荧光强度，但较高 DOS 的蛋白质（例如 DOS > 6）也往往具有较低的荧光强度。大多数抗体的最佳 DOS 建议在 2 到 10 之间，具体取决于染料和蛋白质的特性。为了有效标记，应控制取代度，使 6-8 摩尔 iFluor Ultra 750 SE 对 1 摩尔抗体。以下步骤用于确定 iFluor Ultra 750 SE 标记蛋白质的 DOS。

3.2吸收检测
要检测染料-蛋白质缀合物的吸收光谱，建议将样品浓度保持在 1-10 µM 的范围内，具体取决于染料的消光系数。

3.4计算DOS
您可以通过链接使用我们的工具计算DOS

参考文献

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).
Authors: Seo, Hyesu and Kim, Kyunghee and Park, Woong June
Journal: Biomolecules (2021)

说明书

iFluor Ultra 750 琥珀酰亚胺酯.pdf

iFluor Ultra 750 琥珀酰亚胺酯货号71682-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor Ultra 750 琥珀酰亚胺酯

货号	71682	存储条件	在零下15度以下保存, 避免光照
规格	5 mg	价格	11628
Ex (nm)	749	Em (nm)	773
分子量	1426.78	溶剂	DMSO
产品详细介绍

简要概述

荧光染料缀合抗体是许多应用中鉴定蛋白质的一种工具，包括荧光细胞成像、流式细胞术、蛋白质印迹、免疫组织化学等。使用荧光标记抗体的优势包括更高的灵敏度、多路复用能力和易用性。 iFluor Ultra 系列是我们广受欢迎的 iFluor 染料的最新升级版，并针对用于荧光成像和流式细胞术应用的标记抗体进行了优化。用 iFluor Ultra 750 制备的抗体缀合物远远优于其他现有类似染料的缀合物，如 Alexa Fluor® 750。iFluor Ultra 750 缀合物在相同条件下比用 Alexa Fluor® 750 制备的缀合物更亮。此外，iFluor Ultra 750 的荧光不受 pH (4-10) 的影响。 iFluor Ultra 750 SE 染料相当稳定，并表现出与蛋白质氨基的良好反应性和选择性。 iFluor Ultra 750 的光谱特性和反应性类似于 Alexa Fluor® 750（Alexa Fluor® 是 ThermoFisher 的商标）。

产品说明书

实验方案

储备溶液配制

操作步骤

3.数据处理

3.1表征所需的染料-蛋白质缀合物
取代度 (DOS) 是表征染料标记蛋白质的最重要因素。较低 DOS 的蛋白质通常具有较弱的荧光强度，但较高 DOS 的蛋白质（例如 DOS > 6）也往往具有较低的荧光强度。大多数抗体的最佳 DOS 建议在 2 到 10 之间，具体取决于染料和蛋白质的特性。为了有效标记，应控制取代度，使 6-8 摩尔 iFluor Ultra 750 SE 对 1 摩尔抗体。以下步骤用于确定 iFluor Ultra 750 SE 标记蛋白质的 DOS。

3.2吸收检测
要检测染料-蛋白质缀合物的吸收光谱，建议将样品浓度保持在 1-10 µM 的范围内，具体取决于染料的消光系数。

3.4计算DOS
您可以通过链接使用我们的工具计算DOS

参考文献

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).
Authors: Seo, Hyesu and Kim, Kyunghee and Park, Woong June
Journal: Biomolecules (2021)

说明书

iFluor Ultra 750 琥珀酰亚胺酯.pdf

APC-iFluor 750 串联染料货号2626-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC-iFluor 750 串联染料

货号	2626	存储条件	在2-8度冷藏保存, 避免光照
规格	1 mg	价格	2388
Ex (nm)	754	Em (nm)	776
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：2626

产品名称：APC-iFluor 750 串联染料

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

产品物理化学光谱特性

外观：固体

溶剂：水

产品介绍

串联染料是一类独特的荧光分子，由两种不同的共价连接荧光团、供体（例如 PE 或 APC）和发射较长波长的荧光受体（例如 Texas Red、Cy5、Cy7、iFluor 594 或 iFluor 750组成）。APC-iFluor 750 串联染料是常用 APC-Cy7 的绝佳替代品，具有更高的 FRET 效率和信号。其主要吸收峰位于 651 nm，发射峰位于 ~780 nm。金畔还提供其预活化的 APC-iFluor 750 串联染料，以促进 APC-iFluor 750 串联与抗体和其他蛋白质（如链霉亲和素和其他二级试剂）的串联。我们的预活化 APC-iFluor 750 可随时进行缀合，其产量比传统繁琐的SMCC 的化学方法高得多。此外，我们的预活化 APC-iFluor 750 串联染料通过蛋白质中丰富的氨基与蛋白质缀合，而 SMCC 化学靶向必须通过抗体还原再生的硫醇基团。

参考文献

Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.
Authors: Elezov, D S and Kudryavtsev, I V and Arsent’ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42

Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53

A flow cytometric method for the analysis of macrophages in the vascular wall.
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43

Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77

The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86

Measurement conditions for flow cytometry analyses of cell lines from urological carcinomas.
Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86

An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood.
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

说明书

APC-iFluor 750 串联染料.pdf

APC-AF750 串联染料货号2627-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC-AF750 串联染料

货号	2627	存储条件	在2-8度冷藏保存, 避免光照
规格	1 mg	价格	2388
Ex (nm)	754	Em (nm)	782
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：2627

产品名称：APC-AF750 串联染料

规格：1mg

储存条件：2-8℃避光防潮

保质期：6个月

产品物理化学光谱特性

外观：固体

溶剂：水

产品介绍

串联染料是一类独特的荧光分子，由两种不同的共价连接荧光团、供体（例如 PE 或 APC）和发射较长波长的荧光受体（例如 Texas Red、Cy5、Cy7、iFluor 594 或 iFluor 750组成）。APC-AF750 串联染料具有与常用 APC-Alexa Fluor® 750 相同的结构和特性。其主要吸收峰位于 651 nm，发射峰位于 ~780 nm。

参考文献

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry.
Authors: Rai, S and Bhardwaj, U and Misra, A and Singh, S and Gupta, R
Journal: International journal of laboratory hematology (2018): e52-e54

Dual-Color Fluorescence Imaging of EpCAM and EGFR in Breast Cancer Cells with a Bull’s Eye-Type Plasmonic Chip.
Authors: Izumi, Shota and Yamamura, Shohei and Hayashi, Naoko and Toma, Mana and Tawa, Keiko
Journal: Sensors (Basel, Switzerland) (2017)

Antigen processing of glycoconjugate vaccines; the polysaccharide portion of the pneumococcal CRM(197) conjugate vaccine co-localizes with MHC II on the antigen processing cell surface.
Authors: Lai, Zengzu and Schreiber, John R
Journal: Vaccine (2009): 3137-44

Investigating the role of Natural Killer T-cells in Gram negative infections of patients with type 2 diabetes mellitus.
Authors: Karagianni, P and Polyzos, S A and Bougiouklis, D and Tsapas, A and Paletas, K
Journal: Hippokratia: 231-4

说明书

APC-AF750 串联染料.pdf

dATP 100 mM PCR Grade 货号17250-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

dATP *100 mM PCR Grade*

dATP 100 mM PCR Grade

货号	17250	存储条件	在零下15度以下保存, 避免光照
规格	500 uL	价格	1164
Ex (nm)		Em (nm)
分子量	491.18	溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：17250

产品名称：dATP *100 mM PCR Grade*

CAS：1927-31-7

规格：500ul

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：491.18

外观：液体

溶剂：水

产品介绍

2′-脱氧腺苷 5′-三磷酸钠盐 (dATP) 溶液用于 DNA 合成反应，如 PCR、DNA 测序和分子克隆技术。这种 dATP 溶液适用于需要高质量试剂的许多应用。此类程序包括逆转录 (RT)、聚合酶链反应 (PCR)、RT-PCR、DNA 标记反应和测序/循环测序分析。

参考文献

Development of a Novel Bioluminescence Pyrophosphate Assay for the High-Sensitivity Detection of Hepatitis B Virus.
Authors: Tan, Guolei and Fei, Zhongjie and Wei, Rongbin and Wu, Xuping and Xiao, Pengfeng
Journal: Applied biochemistry and biotechnology (2021)

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2′-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364

Plasmodium falciparum DDX55 is a nucleocytoplasmic protein and a 3′-5′ direction-specific DNA helicase.
Authors: Yasmin, Rahena and Kaur, Inderjeet and Tuteja, Renu
Journal: Protoplasma (2020): 1049-1067

Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP.
Authors: Magriñá, Ivan and Jauset-Rubio, Miriam and Ortiz, Mayreli and Tomaso, Herbert and Simonova, Anna and Hocek, Michal and O’Sullivan, Ciara K
Journal: ACS omega (2019): 21900-21908

Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP.
Authors: Magriñá, Ivan and Toldrà, Anna and Campàs, Mònica and Ortiz, Mayreli and Simonova, Anna and Katakis, Ioanis and Hocek, Michal and O’Sullivan, Ciara K
Journal: Biosensors & bioelectronics (2019): 76-82

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli.
Authors: Singh, Prashant Kumar and Shrivastava, Alok Kumar and Singh, Shilpi and Rai, Ruchi and Chatterjee, Antra and Rai, L C
Journal: Functional & integrative genomics (2017): 39-52

GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small Intestine.
Authors: Thompson, Cayla A and Wojta, Kevin and Pulakanti, Kirthi and Rao, Sridhar and Dawson, Paul and Battle, Michele A
Journal: Cellular and molecular gastroenterology and hepatology (2017): 422-446

说明书

dATP *100 mM PCR Grade*.pdf

iFluor 597 琥珀酰亚胺酯货号1050-AAT Bioquest荧光染料

发表于2022年2月16日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

iFluor 597 琥珀酰亚胺酯

货号	1050	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	3612
Ex (nm)	598	Em (nm)	618
分子量	1058.29	溶剂	DMSO
产品详细介绍

简要概述

iFluor 597琥珀酰亚胺酯是iFluor系列荧光标记染料之一，可以覆盖整个可见光谱。所有iFluor 染料都具有优异的水溶性。它们的亲水性使有机溶剂的使用极小化。与常规的染料(如FITC，TRITC，Texas Red ，Cy3 ，Cy5和Cy7)相比，iFluor 染料具有更好的标记性能。一些iFluor 染料在某些抗体上明显优于Alexa Fluor 标记染料。它们是用于标记蛋白质和核酸的极便宜的荧光染料（替代Alexa Fluor 染料）。每种iFluor染料的开发都与特定的Alexa Fluor 或其他标记染料（如DyLight 染料）的光谱特性相匹配。

琥珀酰亚胺基（NHS）酯被证明是用于胺修饰的极佳试剂，因为形成的酰胺键基本上与天然肽键相同并且稳定。这些试剂通常是稳定的并且与脂族胺显示出良好的反应性和选择性。当琥珀酰亚胺酯化合物用于缀合反应时，需要考虑的因素很少：1.溶剂：在大多数情况下，活性染料应溶于无水二甲基甲酰胺（DMF）或二甲基亚砜（DMSO）中。 2.反应pH：胺与琥珀酰亚胺酯的标记反应强烈依赖于pH。胺反应性试剂与非质子化脂族胺基团反应，包括蛋白质的末端胺和赖氨酸的β-氨基。因此，胺酰化反应通常在pH 7.5以上进行。通过琥珀酰亚胺酯进行的蛋白质修饰通常可以在pH 8.5-9.5下进行。 3.反应缓冲液：使用胺反应试剂时，必须避免使用含有游离胺（如Tris和甘氨酸）和硫醇化合物的缓冲液。广泛用于蛋白质沉淀的铵盐（例如硫酸铵和乙酸铵）也必须在进行染料缀合之前除去（例如通过透析）。 4.反应温度：大多数缀合在室温下进行。特定标记反应可能需要升高或降低的温度。iFluor系列染料是AF系列染料的完美替代品。

iFluor 597 琥珀酰亚胺酯货号1050

AAT Bioquest iFluor 染料干货锦集你想了解的都在这里
锦囊：iFluor 系列染料大集合

产品说明书

染色样本分析

操作步骤

1.准备蛋白质储备溶液（溶液A）：

将100μL反应缓冲液（如1 M碳酸钠溶液或1 M磷酸盐缓冲液，pH~9.0）与900μL目标蛋白溶液（如抗体，蛋白质浓度> 2 mg / ml，如果可能）混合至100μL给予1 mL蛋白质标记原液。

注1：蛋白质溶液（溶液A）的pH值应为8.5±0.5。如果蛋白质溶液的pH低于8.0，则使用1M碳酸氢钠溶液或1M pH 9.0磷酸盐缓冲液将pH调节至8.0-9.0的范围。

注2：蛋白质应溶解于pH7.2-7.4的1X磷酸盐缓冲盐水（PBS）中。如果蛋白质溶解在Tris或甘氨酸缓冲液中，则必须用pH7.2-7.4的1X PBS透析，以除去广泛用于蛋白质沉淀的游离胺或铵盐（例如硫酸铵和乙酸铵）。

注3：不纯的抗体、稳定的牛血清蛋白（BSA）抗体或明胶不会被很好的标记。叠氮化钠或硫柳汞的存在也可能干扰缀合反应。可以通过透析或旋转柱除去叠氮化钠或硫柳汞，以获得极佳标记结果。

注4：如果蛋白质浓度低于2 mg / mL，则结合效率会显着降低。为获得极佳标记效率，建议极终蛋白质浓度范围为2-10 mg / mL。

2.准备染料储备溶液（溶液B）：

将无水DMSO加入到iFluor 染料SE小瓶中以制备10-20mM储备溶液。通过移液或涡旋混合均匀。

注意：在开始缀合前准备染料储备溶液（溶液B）。及时使用。染料储备溶液的长期储存可降低染料活性。溶液B可在冰箱中保存两周，避光保存。避免冻融循环。

3.确定极佳染料/蛋白质比例（可选）：

注意：每种蛋白质都需要不同的染料/蛋白质比例，这也取决于染料的性质。蛋白质的过度标记可能不利地影响其结合亲和力，而低染料/蛋白质比率的蛋白质缀合物会降低灵敏度。我们建议您通过使用连续不同量的标记染料溶液重复步骤4和5来实验确定极佳染料/蛋白质比率。通常，对于大多数染料 – 蛋白质缀合物，推荐使用4-6种染料/蛋白质。

3.1使用10：1摩尔比的溶液B（染料）/溶液A（蛋白质）作为起始点：将5μl染料储备溶液（溶液B，假设染料储备溶液为10 mM）加入到样品瓶中。蛋白质溶液（95μl溶液A）有效摇动。假设蛋白质浓度为10mg / mL并且蛋白质的分子量为~200KD，蛋白质的浓度为~0.05mM。

注意：蛋白质溶液中DMSO的浓度应<10％。

3.2运行缀合反应（参见下面的步骤4）。

3.3重复＃3.2，溶液B /溶液A的摩尔比为5：1;分别为15：1和20：1。

3.4使用预制的旋转柱纯化所需的缀合物。

3.5计算上述4种结合物的染料/蛋白质比（DOS）（见说明书）。

3.6运行上述4种结合物的功能测试，确定极佳的染料/蛋白质比例，以扩大标记反应。

4.运行结合反应：

4.1有效加入适量的染料储备溶液（溶液B）到蛋白质溶液（溶液A）的小瓶中晃动。

注意：溶液B /溶液的极佳摩尔比由步骤3.6确定。如果跳过步骤3，我们建议使用10：1溶液B（染料）/溶液A（蛋白质）的摩尔比。

4.2继续在室温下旋转或摇动反应混合物30-60分钟。

5.纯化缀合物

以下方案是使用Sephadex G-25柱纯化染料 – 蛋白质缀合物的实例。

5.1按照制造说明准备Sephadex G-25色谱柱。

5.2将反应混合物（直接从步骤4）加载到Sephadex G-25柱的顶部。

5.3样品在顶部树脂表面下方运行时立即加入PBS（pH 7.2-7.4）。

5.4向所需样品中加入更多PBS（pH 7.2-7.4）以完成色谱柱纯化。合并含有所需染料 – 蛋白质缀合物的级分。

注1：立即使用时，染料 – 蛋白质偶联物需要用染色缓冲液稀释，并等分多次使用。

注2：对于长期储存，染料 – 蛋白质缀合物溶液需要浓缩或冷冻干燥

参考文献

Comparison of Sensory and Motor Innervation Between the Acupoints LR3 and LR8 in the Rat With Regional Anatomy and Neural Tract Tracing.
Authors: Xu, Dongsheng and Zou, Ling and Zhang, Wenjie and Liao, Jieying and Wang, Jia and Cui, Jingjing and Su, Yuxin and Wang, Yuqing and Guo, Yating and Shen, Yi and Bai, Wanzhu
Journal: Frontiers in integrative neuroscience (2021): 728747

Effect of VIRP1 Protein on Nuclear Import of Citrus Exocortis Viroid (CEVd).
Authors: Seo, Hyesu and Kim, Kyunghee and Park, Woong June
Journal: Biomolecules (2021)

KFP-1, a Novel Calcium-Binding Peptide Isolated from Kefir, Promotes Calcium Influx Through TRPV6 Channels.
Authors: Chang, Gary Ro-Lin and Tu, Min-Yu and Chen, Yu-Hsuan and Chang, Ku-Yi and Chen, Chien-Fu and Lai, Jen-Chieh and Tung, Yu-Tang and Chen, Hsiao-Ling and Fan, Hueng-Chuen and Chen, Chuan-Mu
Journal: Molecular nutrition & food research (2021): e2100182

相关产品

产品名称	货号
iFluor 594马来酰亚胺	Cat#1064

说明书

iFluor 597 琥珀酰亚胺酯.pdf

AF750 NHS 酯与 Alexa Fluor 750 NHS 酯结构相同货号71912-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

AF750 NHS 酯 *与 Alexa Fluor 750 NHS 酯结构相同*

AF750 NHS 酯与 Alexa Fluor 750 NHS 酯结构相同

货号	71912	存储条件	在零下15度以下保存, 避免光照
规格	5 mg	价格	9180
Ex (nm)	752	Em (nm)	776
分子量	~1300	溶剂	DMSO
产品详细介绍

简要概述

产品基本信息

货号：71912

产品名称：AF750 NHS 酯 *与 Alexa Fluor 750 NHS 酯结构相同*

规格：5mg

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：~1300

外观：深蓝色固体

溶剂：DMSO

激发波长(nm)：752

发射波长(nm)：776

产品介绍

AF750 由 AAT Bioquest 制造，具有与 Alexa Fluor® 750 相同的化学结构（Alexa Fluor® 是 ThermoFisher 的商标）。 AF750 是一种鲜红色荧光染料。它是水溶性的，在 pH 4 至 pH 10 范围内对 pH 不敏感。AF750 的 NHS 酯（或琥珀酰亚胺酯）是将这种染料与蛋白质或抗体结合的最方便的胺反应形式。

参考文献

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601

A mouse monoclonal antibody against Alexa Fluor 647
Authors: Wuethrich, I.; Guillen, E.; Ploegh, H. L.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12

Green tea catechins quench the fluorescence of bacteria-conjugated Alexa fluor dyes
Authors: Zhao, L.; Li, W.; Zhu, S.; Tsai, S.; Li, J.; Tracey, K. J.; Wang, P.; Fan, S.; Sama, A. E.; Wang, H.
Journal: Inflamm Allergy Drug Targets (2013): 308-14

Volume labeling with Alexa Fluor dyes and surface functionalization of highly sensitive fluorescent silica (SiO2) nanoparticles
Authors: Wang, W.; Nallathamby, P. D.; Foster, C. M.; Morrell-Falvey, J. L.; Mortensen, N. P.; Doktycz, M. J.; Gu, B.; Retterer, S. T.
Journal: Nanoscale (2013): 10369-75

Fluorescence of Alexa fluor dye tracks protein folding
Authors: Lindhoud, S.; Westphal, A. H.; Visser, A. J.; Borst, J. W.; van Mierlo, C. P.
Journal: PLoS One (2012): e46838

a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594]
Authors: Cui, J. J.; Zhu, X. L.; Ji, C. F.; Jing, X. H.; Bai, W. Z., [Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4
Journal: Zhen Ci Yan Jiu (2011): 262-7

相关产品

产品名称	货号
iFluor 488琥珀酰亚胺酯	Cat#1023
AF 594 NHS酯等同于Alexa Fluor 594	Cat#1830
AF 350 NHS酯等同于Alexa Fluor 350	Cat#1800

说明书

AF750 NHS 酯 *与 Alexa Fluor 750 NHS 酯结构相同*.pdf

TAMRA 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67000-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*

TAMRA 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67000	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

TAMRA 染料 qPCR 校准板可用于校准和维护配备快速 96 孔模块的 7500 实时 PCR 系统。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

Development and validation of a new 18 X-STR typing assay for forensic applications.
Authors: Zhang, Yinming and Yu, Zhengliang and Mo, Xiaoting and Zhao, Xingchun and Li, Wanshui and Liu, Hong and Liu, Chao and Wu, Riga and Sun, Hongyu
Journal: Electrophoresis (2021): 766-773

[Mesenchymal stem cells derived apoptotic extracellular vesicles attenuate pro-inflammatory macrophages induced by Porphyromonas gingivalis lipopolysaccharide].
Authors: Ye, Q Y and Li, Z H and Wang, Y Z and Liu, S Y and Zhou, J and Liu, S Y and Wang, Q T
Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (2021): 791-798

MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.
Authors: Wu, Xiaosong and Pan, Jingyu and Zhu, Xinchao and Hong, Chenggang and Hu, Anzhong and Zhu, Cancan and Liu, Yong and Yang, Ke and Zhu, Ling
Journal: The Analyst (2021): 3823-3833

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027

Double-Stranded DNA Fragments Bearing Unrepairable Lesions and Their Internalization into Mouse Krebs-2 Carcinoma Cells.
Authors: Dolgova, Evgeniya V and Evdokimov, Alexey N and Proskurina, Anastasia S and Efremov, Yaroslav R and Bayborodin, Sergey I and Potter, Ekaterina A and Popov, Alexey A and Petruseva, Irina O and Lavrik, Olga I and Bogachev, Sergey S
Journal: Nucleic acid therapeutics (2019): 278-290

Design and anti-tumor activity of self-loaded nanocarriers of siRNA.
Authors: Han, Wenzhao and Yuan, Ye and Li, Hui and Fu, Zhendong and Wang, Mingyang and Guan, Shuwen and Wang, Liping
Journal: Colloids and surfaces. B, Biointerfaces (2019): 110385

The mechanism and regularity of quenching the effect of bases on fluorophores: the base-quenched probe method.
Authors: Mao, Huihui and Luo, Guanghua and Zhan, Yuxia and Zhang, Jun and Yao, Shuang and Yu, Yang
Journal: The Analyst (2018): 3292-3301

Gene expression profiling of tumor-initiating stem cells from mouse Krebs-2 carcinoma using a novel marker of poorly differentiated cells.
Authors: Potter, Ekaterina A and Dolgova, Evgenia V and Proskurina, Anastasia S and Efremov, Yaroslav R and Minkevich, Alexandra M and Rozanov, Aleksey S and Peltek, Sergey E and Nikolin, Valeriy P and Popova, Nelly A and Seledtsov, Igor A and Molodtsov, Vladimir V and Zavyalov, Evgeniy L and Taranov, Oleg S and Baiborodin, Sergey I and Ostanin, Alexander A and Chernykh, Elena R and Kolchanov, Nikolay A and Bogachev, Sergey S
Journal: Oncotarget (2017): 9425-9441

Detection of Yersinia Enterocolitica Species in Pig Tonsils and Raw Pork Meat by the Real-Time Pcr and Culture Methods.
Authors: Stachelska, M A
Journal: Polish journal of veterinary sciences (2017): 477-484

Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20

说明书

TAMRA 染料 qPCR 校准板*针对 ABI7500 快速 96 孔进行了优化*.pdf

VIC 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67002-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

VIC 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67002	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

VIC 染料 qPCR 校准板可用于维护您的 7500 实时 PCR 系统和快速 96 孔板。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

Rapid screening method of Saccharomyces cerevisiae mutants using calcofluor white and aniline blue.
Authors: Perrine-Walker, Francine and Payne, Jennifer
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2021): 1077-1086

The P2X7 receptor antagonist JNJ-47965567 administered thrice weekly from disease onset does not alter progression of amyotrophic lateral sclerosis in SOD1G93A mice.
Authors: Ly, Diane and Dongol, Anjila and Cuthbertson, Peter and Guy, Thomas V and Geraghty, Nicholas J and Sophocleous, Reece A and Sin, Lucia and Turner, Bradley J and Watson, Debbie and Yerbury, Justin J and Sluyter, Ronald
Journal: Purinergic signalling (2020): 109-122

Development of the MitoQ assay as a real-time quantification of mitochondrial DNA in degraded samples.
Authors: Wai, Ka Tak and Gunn, Peter and Barash, Mark
Journal: International journal of legal medicine (2019): 411-417

Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms.
Authors: Gerdes, Lars and Iwobi, Azuka and Busch, Ulrich and Pecoraro, Sven
Journal: Biomolecular detection and quantification (2016): 9-20

Lineage-specific detection of influenza B virus using real-time polymerase chain reaction with melting curve analysis.
Authors: Tewawong, Nipaporn and Chansaenroj, Jira and Klinfueng, Sirapa and Vichiwattana, Preeyaporn and Korkong, Sumeth and Thongmee, Thanunrat and Theamboonlers, Apiradee and Payungporn, Sunchai and Vongpunsawad, Sompong and Poovorawan, Yong
Journal: Archives of virology (2016): 1425-35

Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22

Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57

The effects of escitalopram on myocardial apoptosis and the expression of Bax and Bcl-2 during myocardial ischemia/reperfusion in a model of rats with depression.
Authors: Wang, Yiming and Zhang, Hongming and Chai, Fangxian and Liu, Xingde and Berk, Michael
Journal: BMC psychiatry (2014): 349

Transcriptional regulators Myb and BCL11A interplay with DNA methyltransferase 1 in developmental silencing of embryonic and fetal β-like globin genes.
Authors: Roosjen, Mark and McColl, Bradley and Kao, Betty and Gearing, Linden J and Blewitt, Marnie E and Vadolas, Jim
Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014): 1610-20

Identification of an isogenic semidwarf rice cultivar carrying the Green Revolution sd1 gene by multiplex codominant ASP-PCR and SSR markers.
Authors: Naito, Yoshiki and Tomita, Motonori
Journal: Biochemical genetics (2013): 530-42

说明书

VIC 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

ROX 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67004-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

ROX 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67004	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

ROX 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600

RT-qPCR Detection of Low-Copy HIV RNA with Yin-Yang Probes.
Authors: Kireev, Dmitry E and Farzan, Valentina M and Shipulin, German A and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 27-35

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027

Developmental validation of the Microreader™ 20A ID system.
Authors: Qu, Shengqiu and Li, Hang and Li, Yifan and Lv, Meili and Yang, Fan and Zhu, Jing and Yu, Zailiang and Liu, Yuqing and Chen, Chuguang and Wang, Yinji and Li, Zhuo and Zhang, Lin and Liang, Weibo
Journal: Electrophoresis (2019): 3099-3107

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

说明书

ROX 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

FAM 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67006-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67006	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

FAM 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

Novel design of nucleic acid standards for hydrolysis probe-based PCR with melting analysis.
Authors: Baoutina, Anna and Bhat, Somanath
Journal: Gene therapy (2021)

Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel.
Authors: Zhang, Haoqing and Yan, Zhiqiang and Wang, Xinlu and Gaňová, Martina and Chang, Honglong and Laššáková, Soňa and Korabecna, Marie and Neuzil, Pavel
Journal: ACS omega (2021): 22292-22300

SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600

Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity.
Authors: Menendez-Gil, Pilar and Caballero, Carlos J and Solano, Cristina and Toledo-Arana, Alejandro
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 41-58

Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis.
Authors: Nyaruaba, Raphael and Xiong, Jin and Mwaliko, Caroline and Wang, Nuo and Kibii, Belindah J and Yu, Junping and Wei, Hongping
Journal: Microorganisms (2020)

A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773

Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions.
Authors: Tomé, Stéphanie and Gourdon, Geneviève
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 11-23

Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection.
Authors: Wang, Zhenzhen and Xue, Zhiqiang and Hao, Xiaoli and Miao, Chenfang and Zhang, Jianzhong and Zheng, Yanjie and Zheng, Zongfu and Lin, Xinhua and Weng, Shaohuang
Journal: Analytica chimica acta (2020): 212-219

PharmFrag: An Easy and Fast Multiplex Pharmacogenetics Assay to Simultaneously Analyze 9 Genetic Polymorphisms Involved in Response Variability of Anticancer Drugs.
Authors: Bouvet, Régis and Verdier, Marie-Clémence and El Baroudi, Yahya and Galibert, Marie-Dominique and David, Véronique and Schutz, Sacha and Tron, Camille
Journal: International journal of molecular sciences (2020)

说明书

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

SYBR 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67008-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

SYBR 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67008	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

SYBR 染料 qPCR 校准板可用于维护配备 Fast 96 孔板的 7500 实时 PCR 系统。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

Combined evaluation of proliferation and apoptosis to calculate IC50 of VPA-induced PANC-1 cells and assessing its effect on the Wnt signaling pathway.
Authors: Ekici, Yeliz and Yilmaz, Abdullah and Kucuksezer, Umut Can and Gazioglu, Sema Bilgic and Yamalioglu, Zeynep Dogusan and Gurol, Ali Osman and Linn, Thomas and Tuncer, Feyza Nur
Journal: Medical oncology (Northwood, London, England) (2021): 109

Identification of Echinococcus granulosus Genotypes G1 and G3 by SNPs Genotyping Assays.
Authors: Bonelli, Piero and Dei Giudici, Silvia and Peruzzu, Angela and Mura, Lorena and Santucciu, Cinzia and Maestrale, Caterina and Masala, Giovanna
Journal: Pathogens (Basel, Switzerland) (2021)

Expression levels of serum circulating microRNAs in pediatric patients with ventricular and supraventricular arrhythmias.
Authors: Moric-Janiszewska, Ewa and Smolik, Sławomir and Morka, Aleksandra and Szydłowski, Lesław and Kapral, Małgorzata
Journal: Advances in medical sciences (2021): 411-417

RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors.
Authors: Munawar, Mustafa Ahmad and Martin, Frank and Toljamo, Anna and Kokko, Harri and Oksanen, Elina
Journal: BioTechniques (2020): 270-280

Rapid detection of porcine DNA in processed food samples using a streamlined DNA extraction method combined with the SYBR Green real-time PCR assay.
Authors: Tan, Lee Lee and Ahmed, Siti Aminah and Ng, Siew Kit and Citartan, Marimuthu and Raabe, Carsten A and Rozhdestvensky, Timofey S and Tang, Thean Hock
Journal: Food chemistry (2020): 125654

Expression of SARS-CoV-2 receptor ACE2 and TMPRSS2 in human primary conjunctival and pterygium cell lines and in mouse cornea.
Authors: Ma, Di and Chen, Chong-Bo and Jhanji, Vishal and Xu, Ciyan and Yuan, Xiang-Ling and Liang, Jia-Jian and Huang, Yuqiang and Cen, Ling-Ping and Ng, Tsz Kin
Journal: Eye (London, England) (2020): 1212-1219

Preoperative heat shock protein 47 levels identify colorectal cancer patients with lymph node metastasis and poor prognosis.
Authors: Mori, Koichiro and Toiyama, Yuji and Okugawa, Yoshinaga and Ichikawa, Takashi and Nagano, Yuka and Oki, Satoshi and Shimura, Tadanobu and Fujikawa, Hiroyuki and Hiro, Junichiro and Kobayash, Minako and Araki, Toshimitsu and Inoue, Yasuhiro and Mohri, Yasuhiko and Kusunoki, Masato
Journal: Oncology letters (2020): 333

Distribution of virulence genes in bacteremic methicillin-resistant Staphylococcus aureus isolates from various sources.
Authors: Wang, Fu-Der and Wu, Ping-Feng and Chen, Su-Jung
Journal: Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi (2019): 426-432

Polycystic ovary syndrome dependency on mtDNA mutation; copy Number and its association with insulin resistance.
Authors: Saeed, Noor AlHuda Ali A H and Hamzah, Israa Hussein and Al-Gharrawi, Samar Abdul Raheem
Journal: BMC research notes (2019): 455

Aberrant Expression of the miR-181b/miR-222 after Hematopoietic Stem Cell Transplantation in Patients with Acute Myeloid Leukemia.
Authors: Iravani Saadi, Mahdiyar and Arandi, Nargess and Yaghobi, Ramin and Azarpira, Negar and Geramizadeh, Bita and Ramzi, Mani
Journal: Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion (2019): 446-450

说明书

SYBR 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

NED 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67010-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

NED 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67010	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

NED 染料 qPCR 校准板可用于维护您的 7500 实时 PCR 系统和快速 96 孔板。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

DNA degradation in human teeth exposed to thermal stress.
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118

Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82

Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22

Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57

An improved rapid quantitative detection and identification method for a wide range of fungi.
Authors: Soeta, Nobutoshi and Terashima, Masanori and Gotoh, Mitsukazu and Mori, Shuichi and Nishiyama, Kyoko and Ishioka, Ken and Kaneko, Hisatoshi and Suzutani, Tatsuo
Journal: Journal of medical microbiology (2009): 1037-1044

Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36

TaqMan reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Medeiros, L Jeffrey
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 135-45

TaqMan RT-PCR assay coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Sanchez-Vega, Beatriz and Medeiros, L Jeffrey
Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2004): 96-103

Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses.
Authors: Dimsoski, Pero
Journal: Croatian medical journal (2003): 332-5

Semiquantitative and qualitative assessment of B-lymphocyte V H repertoire by a fluorescent multiplex PCR.
Authors: Feuchtenberger, Martin and Tony, Hans-Peter and Rouzière, Anne-Sophie and Jacobi, Anette and Dörner, Thomas and Kneitz, Christian and Starostik, Petr
Journal: Journal of immunological methods (2003): 121-7

说明书

NED 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

JOE 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67012-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67012	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

JOE 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81

Molecular beacons with JOE dye: Influence of linker and 3′ couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290

Mineralization Effect of Hyaluronan on Dental Pulp Cells via CD44.
Authors: Chen, Kuan-Liang and Yeh, Ying-Yi and Lung, Jrhau and Yang, Yu-Chi and Yuan, Kuo
Journal: Journal of endodontics (2016): 711-6

Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220

Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56

Combined effects of simvastatin and enamel matrix derivative on odontoblastic differentiation of human dental pulp cells.
Authors: Karanxha, Lorena and Park, Su-Jung and Son, Won-Jun and Nör, Jacques E and Min, Kyung-San
Journal: Journal of endodontics (2013): 76-82

In vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time PCR.
Authors: Kim, Sin-Young and Shin, Yooseok and Lee, Chan-Young and Jung, Il-Young
Journal: Journal of endodontics (2013): 1359-63

Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqMan™ probe.
Authors: Ryazantsev, Dmitry Y and Tsybulsky, Dmitry A and Prokhorenko, Igor A and Kvach, Maksim V and Martynenko, Yury V and Philipchenko, Pavel M and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Analytical and bioanalytical chemistry (2012): 59-68

ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
Authors: Zhao, Zheng and Liu, Hongchen and Wang, Dongsheng
Journal: Journal of endodontics (2011): 332-9

说明书

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

7 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67020-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

7 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67020	存储条件	在零下15度以下保存, 避免光照
规格	1 Set	价格	11400
Ex (nm)		Em (nm)
分子量		溶剂
产品详细介绍

简要概述

7 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。对于大多数 qPCR 仪器，必要的校准应至少每六个月进行一次。该校准板可以立即使用，无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

参考文献

Evaluation of the Thermo Scientific SureTect Salmonella Species PCR Assay in a Broad Range of Foods and Select Environmental Surfaces: Pre-Collaborative and Collaborative Study: First Action 2021.02.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Leonte, Ana-Maria and Vandoros, Evangelos J and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022): 167-190

Development and Evaluation of a TaqMan Real-Time PCR Assay for the Rapid Detection of Cross-Contamination of RD (Human) and L20B (Mouse) Cell Lines Used in Poliovirus Surveillance.
Authors: Ahmad, Ausaf and Lee, Joo R and Metz, John M and Tang, Xiaoling and Lin, Seh-Ching and Bagarozzi, Dennis A and Petway, David and Herzegh, Owen
Journal: Journal of virological methods (2022): 114354

Evaluation of the Thermo ScientificTM SureTectTMListeria monocytogenes PCR Assay in a Broad Range of Foods and Selected Environmental Surfaces: Pre-Collaborative and Collaborative Study, First Action 2021.05.
Authors: Bastin, Benjamin and Thompson, Wesley and Benzinger, M Joseph and Crowley, Erin S and Vandoros, Evangelos J and Leonte, Ana-Maria and Thomas, Daniel and Hughes, Annette and Crabtree, David and Evans, Katharine and Sohier, Daniele
Journal: Journal of AOAC International (2022)

The Comparison of Real-time-PCR-HRM and Microscopy Methods for Detection of Mixed Plasmodium spp. Infections in Laghman Province, Afghanistan.
Authors: Dalimi, Abdolhossein and Mosawi, Sayed Hussain
Journal: Infectious disorders drug targets (2021): 399-404

Clinical evaluation of a multiplex real-time RT-PCR assay for detection of SARS-CoV-2 in individual and pooled upper respiratory tract samples.
Authors: Laverack, Melissa and Tallmadge, Rebecca L and Venugopalan, Roopa and Cronk, Brittany and Zhang, XiuLin and Rauh, Rolf and Saunders, Amy and Nelson, William M and Plocharczyk, Elizabeth and Diel, Diego G
Journal: Archives of virology (2021): 2551-2561

TaqMan real time PCR for the Detection of the Gilbert’s Syndrome Markers UGT1A1*28; UGT1A1*36 and UGT1A1*37.
Authors: Daprà, Valentina and Alliaudi, Carla and Galliano, Ilaria and Dini, Maddalena and Curcio, Giada Lo and Calvi, Cristina and Archetti, Marialaura and Gavatorta, Martina and Bergallo, Massimiliano
Journal: Molecular biology reports (2021): 4953-4959

Rapid and Safe Detection of SARS-CoV-2 and Influenza Virus RNA Using Onsite Quantitative PCR Diagnostic Testing From Clinical Specimens Collected in Molecular Transport Medium.
Authors: Daum, Luke T and Fischer, Gerald W
Journal: The journal of applied laboratory medicine (2021): 1409-1416

Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay.
Authors: Potter, Robert F and Abro, Brooj and Eby, Charles S and Burnham, Carey-Ann D and Anderson, Neil W and Parikh, Bijal A
Journal: Diagnostic microbiology and infectious disease (2021): 115387

Validation of the Thermo Scientific™ SureTect™ Staphylococcus aureus PCR Assay for the Detection of Staphylococcus aureus in Dairy Matrices: AOAC Performance Tested MethodsSM 052101.
Authors: Evans, Katharine and Faulds, Nikki and Crabtree, David and Hughes, Annette and Sohier, Daniele and Manthe, Craig and Hahs, Matthew and Heikkinen, Pauliina and Hurskainen, Emmi and Koch, Kateland and Thompson, Wesley and Bastin, Benjamin and Benzinger, M Joseph
Journal: Journal of AOAC International (2021)

Comparison of Two Commercial Molecular Tests and a Laboratory-Developed Modification of the CDC 2019-nCoV Reverse Transcriptase PCR Assay for the Detection of SARS-CoV-2.
Authors: Moore, Nicholas M and Li, Haiying and Schejbal, Debra and Lindsley, Jennifer and Hayden, Mary K
Journal: Journal of clinical microbiology (2020)

说明书

7 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

Cy3.5 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化货号67014-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

Cy3.5 染料 qPCR 校准板针对 ABI7500 快速 96 孔进行了优化

货号	67014	存储条件	在零下15度以下保存, 避免光照
规格	1 Plate	价格	1140
Ex (nm)		Em (nm)
分子量		溶剂	DMSO
产品详细介绍

简要概述

产品基本信息

货号：67014

产品名称：Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

规格：1 Plate

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

外观：液体

溶剂：DMSO

产品介绍

Cy3.5 Dye qPCR 校准板可用于维护您的 7500 Real-Time PCR 系统和快速 96 孔模块。对于大多数 qPCR 仪器，必须至少每六个月校准一次设备。该校准板无需任何额外的准备步骤即可使用。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来显着改善多路复用的 qPCR 结果。有关详细的校准操作，请参阅您的仪器指南。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cy3.5 染料 qPCR 校准板。

参考文献

Development of a rapid qPCR method to quantify lactic acid bacteria in cold-smoked salmon.
Authors: Jérôme, Marc and Passerini, Delphine and Chevalier, Frédérique and Marchand, Laetitia and Leroi, Françoise and Macé, Sabrina
Journal: International journal of food microbiology (2022): 109504

Semi-Quantitative Detection of Drosophila suzukii (Diptera: Drosophilidae) From Bulk Trap Samples Using PCR Technology.
Authors: Renkema, Justin M and McFadden-Smith, Wendy and Chen, Shu
Journal: Journal of economic entomology (2022)

Continuous polymerase chain reaction microfluidics integrated with a gold-capped nanoslit sensing chip for Epstein-Barr virus detection.
Authors: Hsieh, Han-Yun and Chang, Ray and Huang, Yung-Yu and Juan, Po-Han and Tahara, Hidetoshi and Lee, Kuan-Yi and Vo, Di Ngoc Kha and Tsai, Ming-Han and Wei, Pei-Kuen and Sheen, Horn-Jiunn and Fan, Yu-Jui
Journal: Biosensors & bioelectronics (2022): 113672

XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway.
Authors: Zhang, Yuan and Chen, Xiaolan and Fan, Yaping and Liu, Jing and Yuan, Li
Journal: Nephron (2022): 84-98

DNA-based quantification of Fusarium oxysporum f. sp. vasinfectum in environmental soils to describe spatial variation in inoculum density.
Authors: Davis, Roy and Isakeit, Thomas and Chappell, Thomas
Journal: Plant disease (2022)

First case of SARS-CoV-2 RNA detection in municipal solid waste leachate from Brazil.
Authors: Mondelli, Giulliana and Silva, Ednei Rodrigues and Claro, Ieda Carolina Mantovani and Augusto, Matheus Ribeiro and Duran, Adriana Feliciano Alves and Cabral, Aline Diniz and de Moraes Bomediano Camillo, Lívia and Dos Santos Oliveira, Luísa Helena and de Freitas Bueno, Rodrigo
Journal: The Science of the total environment (2022): 153927

Precise RNA Quantification by Counting Individual RNA Molecules Using High-Sensitivity Capillary Flow Cytometry.
Authors: Yoo, Hee-Bong and Park, Sang-Ryoul and Hong, Kee-Suk and Yang, Inchul
Journal: Analytical chemistry (2022): 1752-1759

Accurate qPCR quantification in polymicrobial communities requires assessment of gDNA extraction efficiency.
Authors: Cerca, Nuno and Lima, Ângela and França, Angela
Journal: Journal of microbiological methods (2022): 106421

Lentiviral standards to determine the sensitivity of assays that quantify lentiviral vector copy numbers and genomic insertion sites in cells.
Authors: Corre, Guillaume and Seye, Ababacar and Frin, Sophie and Ferrand, Maxime and Winkler, Kathrin and Luc, Cyril and Dorange, Fabien and Rocca, Céline J and Galy, Anne
Journal: Gene therapy (2022)

One-Step Multiplexed Droplet Digital Polymerase Chain Reaction for Quantification of p190 BCR-ABL1 Fusion Transcript in B-Lymphoblastic Leukemia.
Authors: Martinez, Ryan J and Kang, Qing and Nennig, Davis and Bailey, Nathanael G and Brown, Noah A and Betz, Bryan L and Tewari, Muneesh and Thyagarajan, Bharat and Bachanova, Veronika and Mroz, Pawel
Journal: Archives of pathology & laboratory medicine (2022): 92-100

说明书

Cy3.5 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

LAMP Green™ 50X DMSO 溶液货号17555-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

LAMP Green™ *50X DMSO 溶液*

LAMP Green™ 50X DMSO 溶液

货号	17555	存储条件	在零下15度以下保存, 避免光照
规格	100 ul	价格	3540
Ex (nm)		Em (nm)
分子量	578.73	溶剂	DMSO
产品详细介绍

简要概述

等温扩增方法以流线型、指数方式提供核酸靶序列的检测。现有成功检测 LAMP 反应的染料大多基于使用比色分析的终点法。 LAMP Green 可以对 LAMP 反应进行实时荧光测量。可以使用普通实时 PCR 仪器上的 SYBR 或 FAM 通道检测染料。 LAMP Green 与琼脂糖凝胶电泳兼容，可以在凝胶上运行并通过凝胶成像仪进行分析。

适用仪器

qPCR
仪器规格：	SYBR 或 FAM 滤波片
荧光酶标仪
Ex(nm):	490
Em(nm):	525
cutoff:	515
推荐孔板：	纯黑色孔板

产品说明书

样品实验方案

表 1. 25 μL LAMP 反应混合物

成分	DNA/RNA 目标检测	无模板对照（NTC）
LAMP master mix (2X）	12.5ul	12.5ul
LAMP primer mix (10X)	2.5ul	2.5ul
LAMP Green (50X)	0.5ul	0.5ul
Target DNA/RNA	Varies	0ul
ddH2O	Varies	9.5ul
总容积	25ul	25ul

以下方案可用作指导，应根据您的实验需要进行优化

1.在室温下解冻所有要使用的组件并放在冰上。短暂涡旋混合并离心以收集材料。

2.如表 1 所述制备反应混合物。列出了 25 μL/LAMP 反应的体积。根据使用情况相应调整体积。如有必要，可以优化 LAMP Green 浓度。

3.涡旋反应混合物和离心机收集材料。

4.密封反应容器。

5.在 65°C 下孵育 30 分钟。如有必要，可以延长时间（即，对于低拷贝目标、具有挑战性的样品类型或已知会产生较慢扩增时间的反应）

6.如果使用 qPCR仪，可以使用 SYBR 或 FAM 滤波片实时读取信号。对于基于酶标仪的检测，在 Ex/Em = 490/525 nm（Cutoff = 515 nm）处测量信号

7.如果在 LAMP 反应完成后分析反应产物，则通过在 >80°C 下加热 5 分钟使酶失活。

注意：LAMP Green 与琼脂糖凝胶电泳兼容，因此可以在琼脂糖凝胶上分析样品。

图示

LAMP Green™ *50X DMSO 溶液* 货号17555

图1. HeLa 细胞中 BRCA1 的 LAMP 检测。使用 ABI 7500 qPCR 仪将 HeLa 细胞中的 500 ng（绿色）、50 ng（蓝色）、5 ng（橙色）和 NTC（黑色）的 gDNA 用于与 LAMP Green 荧光染料的 LAMP 反应

说明书

LAMP Green™ *50X DMSO 溶液*.pdf

ReadiCleave Cy5-SSL-NHS 酯货号7050-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiCleave Cy5-SSL-NHS 酯

货号	7050	存储条件	在零下15度以下保存, 避免光照
规格	1 mg	价格	4740
Ex (nm)	651	Em (nm)	670
分子量	1018.33	溶剂	DMSO
产品详细介绍

简要概述

产品基本信息

货号：7050

产品名称：ReadiCleave Cy5-SSL-NHS 酯

规格：1mg

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：1018.33

外观：固体

溶剂：DMSO

激发波长(nm)：651

发射波长(nm)：670

产品介绍

基于荧光的方法在灵敏度和方便性方面对生物检测具有许多优势。许多生物分子可以很容易地用荧光标签标记，用于荧光成像和流式细胞术分析。然而，大多数现有的荧光标签用于永久标记生物目标，添加的荧光标签不能从这些生物目标上切割下来，以进行进一步的下游分析，例如质谱分析或其他检测模式。 AAT Bioquest 的 ReadiCleave 探针使荧光标签能够与生物靶标结合，在需要时可以从该靶标上去除添加的荧光标签。 ReadiCleave Cy5-SSL-NHS 酯包含一个 S-S 探针，可以用 TCEP 或 DTT 切割以从目标分子中去除 Cy5 荧光团。裂解可通过加入 DTT 或 TCEP 溶液并从室温至 65°C 孵育 1-10 分钟来进行。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiCleave Cy5-SSL-NHS 酯。

点击查看光谱

参考文献

Repurposing an atherosclerosis targeting peptide for tumor imaging.
Authors: Kovacs, Luciana and Davis, Ryan A and Ganguly, Tanushree and Chammas, Roger and Sutcliffe, Julie L
Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2022): 112469

Small molecule based EGFR targeting of biodegradable nanoparticles containing temozolomide and Cy5 dye for greatly enhanced image-guided glioblastoma therapy.
Authors: Schmitt, Rebecca R and Mahajan, Supriya D and Pliss, Artem and Prasad, Paras N
Journal: Nanomedicine : nanotechnology, biology, and medicine (2022): 102513

Nucleic Acid-Gated Covalent Organic Frameworks for Cancer-Specific Imaging and Drug Release.
Authors: Gao, Peng and Shen, Xiaoying and Liu, Xiaohan and Chen, Yuanyuan and Pan, Wei and Li, Na and Tang, Bo
Journal: Analytical chemistry (2021): 11751-11757

Near-Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette.
Authors: Abe, Masahiro and Nishihara, Ryo and Kim, Sung-Bae and Suzuki, Koji
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 103-110

Optimizing the Biodistribution of Radiofluorinated Barbiturate Tracers for Matrix Metalloproteinase Imaging by Introduction of Fluorescent Dyes as Pharmacokinetic Modulators.
Authors: Schwegmann, Katrin and Hohn, Michael and Hermann, Sven and Schäfers, Michael and Riemann, Burkhard and Haufe, Günter and Wagner, Stefan and Breyholz, Hans-Jörg
Journal: Bioconjugate chemistry (2020): 1117-1132

Targeting and imaging colorectal cancer by activatable cell-penetrating peptides.
Authors: Zeng, Ziwei and Chen, Junji and Luo, Shuangling and Dong, Jianghui and Hu, Huanxin and Yang, Zihuan and Feng, Xingzhi and Liu, Yiting and Liu, Binbin and Pan, Guangyu and Zhou, Fiona H and Wang, Liping and Kang, Liang
Journal: American journal of translational research (2020): 1754-1766

Simultaneous and ultrasensitive detection of multiple microRNAs by single-molecule fluorescence imaging.
Authors: Zhang, Hongding and Huang, Xuedong and Liu, Jianwei and Liu, Baohong
Journal: Chemical science (2020): 3812-3819

A split aptamer sensing platform for highly sensitive detection of theophylline based on dual-color fluorescence colocalization and single molecule photobleaching.
Authors: Liu, Shi Gang and Zhang, Dong and He, Yu and Gao, Wenli and Shi, Xingbo
Journal: Biosensors & bioelectronics (2020): 112461

Hybrid Tracers Based on Cyanine Backbones Targeting Prostate-Specific Membrane Antigen: Tuning Pharmacokinetic Properties and Exploring Dye-Protein Interaction.
Authors: Hensbergen, Albertus W and Buckle, Tessa and van Willigen, Danny M and Schottelius, Margret and Welling, Mick M and van der Wijk, Felicia A and Maurer, Tobias and van der Poel, Henk G and van der Pluijm, Gabri and van Weerden, Wytske M and Wester, Hans-Jürgen and van Leeuwen, Fijs W B
Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine (2020): 234-241

说明书

ReadiCleave Cy5-SSL-NHS 酯.pdf

ReadiUse MTS 试剂 50 mM 水溶液货号15710-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse MTS 试剂 *50 mM 水溶液*

ReadiUse MTS 试剂 50 mM 水溶液

货号	15710	存储条件	在2-8度冷藏保存, 避免光照
规格	5 mL (122 mg)	价格	2100
Ex (nm)		Em (nm)
分子量	487.51	溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：15710

产品名称：ReadiUse MTS 试剂 *50 mM 水溶液*

规格：5 mL (122 mg)

储存条件：2-8℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：487.51

外观：液体

溶剂：水

产品介绍

MTS 试剂是最常见的水溶性四唑鎓试剂之一，广泛用于通过吸收分光光度法定量细胞活力。多种四唑内盐用于通过检测它们的颜色变化来确定增殖实验中的细胞活力。代谢活性细胞将无色四唑内盐还原为有色甲臜产物，根据四唑试剂的结构，这些产物可能在细胞培养基中可溶或不溶。四唑内盐向甲臜的转化被认为是通过 NAD(P)H 依赖性脱氢酶进行的。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiUse MTS 试剂 *50 mM 水溶液*。

参考文献

The edge effect: A global problem. The trouble with culturing cells in 96-well plates.
Authors: Mansoury, Morva and Hamed, Maya and Karmustaji, Rashid and Al Hannan, Fatima and Safrany, Stephen T
Journal: Biochemistry and biophysics reports (2021): 100987

Profiling the Neoplasm Microenvironment of Silica Nanomaterial-Derived Scaffolds of Single, 2-, and 3-Composite Systems.
Authors: Akpe, Victor and Murhekar, Shweta and Kim, Tak H and Brown, Christopher L and Cock, Ian E
Journal: Assay and drug development technologies (2021): 191-203

A Modified MTS Proliferation Assay for Suspended Cells to Avoid the Interference by Hydralazine and β-Mercaptoethanol.
Authors: Wang, Yutang and Nguyen, Dinh Tam and Yang, Guang and Anesi, Jack and Kelly, Jason and Chai, Zhonglin and Ahmady, Fahima and Charchar, Fadi and Golledge, Jonathan
Journal: Assay and drug development technologies (2021): 184-190

Synthesis, structural characterization, and prospects for new cobalt (II) complexes with thiocarbamoyl-pyrazoline ligands as promising antifungal agents.
Authors: Dias, Bianca Boni and da Silva Dantas, Fabiana Gomes and Galvão, Fernanda and Cupozak-Pinheiro, Wellinton Jhon and Wender, Heberton and Pizzuti, Lucas and Rosa, Persiely Pires and Tenório, Kátia Veronica and Gatto, Claudia Cristina and Negri, Melyssa and Casagrande, Gleison Antônio and de Oliveira, Kelly Mari Pires
Journal: Journal of inorganic biochemistry (2020): 111277

The Predictive Value of Estrogen Receptor 1 on Adjuvant Chemotherapy in Locally Advanced Colorectal Cancer: A Retrospective Analysis With Independent Validation and Its Potential Mechanism.
Authors: Ye, Shu-Biao and Cheng, Yi-Kan and Deng, Ru and Deng, Yanhong and Li, Peisi and Zhang, Lin and Lan, Ping
Journal: Frontiers in oncology (2020): 214

Calcineurin/NFAT Signaling Modulates Pulmonary Artery Smooth Muscle Cell Proliferation, Migration and Apoptosis in Monocrotaline-Induced Pulmonary Arterial Hypertension Rats.
Authors: He, Rui-Lan and Wu, Zhi-Juan and Liu, Xiao-Ru and Gui, Long-Xin and Wang, Rui-Xing and Lin, Mo-Jun
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2018): 172-189

Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules.
Authors: Tong, Xuhui and Lopez, William and Ramachandran, Jayalakshmi and Ayad, Wafaa A and Liu, Yu and Lopez-Rodriguez, Angelica and Harris, Andrew L and Contreras, Jorge E
Journal: The Journal of general physiology (2015): 245-54

Caution is required in interpretation of mutations in the voltage sensing domain of voltage gated channels as evidence for gating mechanisms.
Authors: Kariev, Alisher M and Green, Michael E
Journal: International journal of molecular sciences (2015): 1627-43

Cysteine accessibility probes timing and extent of NBD separation along the dimer interface in gating CFTR channels.
Authors: Chaves, Luiz A Poletto and Gadsby, David C
Journal: The Journal of general physiology (2015): 261-83

CHARMM-GUI PDB manipulator for advanced modeling and simulations of proteins containing nonstandard residues.
Authors: Jo, Sunhwan and Cheng, Xi and Islam, Shahidul M and Huang, Lei and Rui, Huan and Zhu, Allen and Lee, Hui Sun and Qi, Yifei and Han, Wei and Vanommeslaeghe, Kenno and MacKerell, Alexander D and Roux, Benoît and Im, Wonpil
Journal: Advances in protein chemistry and structural biology (2014): 235-65

说明书

ReadiUse MTS 试剂 *50 mM 水溶液*.pdf

ReadiUse MTS 试剂 50 mM 水溶液货号15711-AAT Bioquest荧光染料

发表于2022年2月15日由Harlan Teklad中国

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

ReadiUse MTS 试剂 *50 mM 水溶液*

ReadiUse MTS 试剂 50 mM 水溶液

货号	15711	存储条件	在2-8度冷藏保存, 避免光照
规格	50 mL (1.22 g)	价格	15000
Ex (nm)		Em (nm)
分子量	487.51	溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：15711

产品名称：ReadiUse MTS 试剂 *50 mM 水溶液*

规格：50 mL (1.22 g)

储存条件：2-8℃避光防潮

保质期：12个月

产品物理化学光谱特性

分子量：487.51

外观：液体

溶剂：水

产品介绍

参考文献

说明书

ReadiUse MTS 试剂 *50 mM 水溶液*.pdf