Individual Detergent Screen HT Reagents蛋白结晶试剂盒Hampton Research

发表于2024年12月6日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Custom Shop Crystallization Reagents > Detergent Screen HT > Individual Detergent Screen HT Reagents

Individual Detergent Screen HT Reagents

Applications

Individual Detergent Screen reagents for screening and optimization

Features

0.5 ml volume in a sterile polypropylene tune with o-ring seal.
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-406-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-406-01

Individual reagents from the original HR2-406 Detergent Screen HT supplied in a volume of 0.5 milliliter in a sterile polypropylene tune with o-ring seal. All detergents formulated in deionized, sterile filtered Type 1 Laboratory Water. Container is purged with argon prior to closure.

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CAT NO

HR2-406-92

Polyethylene glycol 10,000蛋白结晶试剂盒Hampton Research

发表于2024年12月5日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 10,000

Polyethylene glycol 10,000

Applications

Crystallization grade Polyethylene glycol 10,000 for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Polyethylene glycol 10,000

Synonyms: PEG 10,000
H(OCH₂CH₂)_nOH
M_r 8,500 – 11,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4110000
MDL Number MFCD00081839
PubChem Substance ID 24887732

Aldehyde None detected
Peroxide None detected

Measured Conductivity Range: 38.2 – 48.3 µS/cm at 25°C
Measured Refractive Index Range: 1.40165 – 1.40271 at 20°C

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CAT NO

HR2-607

NAME

50% w/v Polyethylene glycol 10,000

DESCRIPTION

200 mL

PRICE

$100.00

cart quote

Support Material(s)

HR2-607 Polyethylene glycol 10,000 SDS

Certificate Of Analysis

References

1. Crystal structure of a catalytic antibody Fab with esterase-like activity. Golinelli-Pimpaneau, B; Gigant, B; Bizebard, T; Navaza, J; Saludjian, P; Zemel, R; Tawfik, DS; Eshhar, Z; Green, BS; Knossow, M. Structure 2, 175- 183, 1994.

Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).

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3 Well Midi UVXPO蛋白结晶试剂盒Hampton Research

发表于2024年12月5日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > 96 Well Crystallization Plates > Swissci > 3 Well Midi UVXPO

3 Well Midi UVXPO

Applications

Sitting drop crystallization

Features

Lens effect drop wells for enhanced optics
Drop volume: 50 nl to 5 µl
Micro-numbering alongside drop volumes
Reservoir volume: 20 to 40 µl
ANSI/SLAS 1-2004 Standard
Drop well allows easier crystal harvesting
Three drop wells per reservoir
Midi profile for easier storage
UV compatible

Description

The SWISSCI 3 Lens Crystallization Plate is an ideal product for protein crystallization presented in a 96-well plate format.

The SWISSCI UVXPO 3 Lens Crystallisation Plate is made from a new polymer mixture that means crystals can be viewed in UV, Polar and Visible light. UVXPO is short for UV compatible as well as excellent uniform (zero) background when using cross polarised light.

The SWISSCI 3 Lens Crystallisation Plate is an ideal product for protein crystallisation presented in a 96-well plate format.

The Advantages of the 3 Lens Crystallization Plate

Easy crystal retrieval
Raised wide wells make the crystal mounting especially easy.

Easy viewing
Crystals can be viewed in UV, Polar and Visible light.
Uniform (zero) background when using cross polarised light.
The wells are a wide conical shape and have a lens effect for perfect illumination.
The micro-numbering ensures you will never get lost again (visible by microscope).
The optically superior polymer is UV transmissible and may be used to differentiate between salt and protein crystals.

Better sealing
Wide partition walls between the wells give plenty of area for good sealing with tape.
No central bending occurs in this very robust structure.
Excellent long term storage – no sample evaporation.

Wide range of volumes
Typical volumes are 20-40 µl of reservoir and 50 nl-5 µl drop size. The 288 optical wells offer three times the number of experimental constructions.

ANSI/SLAS 1-2004 Standard
The plates are designed to the 96-well ANSI/SLAS 1-2004 standard for all common holders and external numbering (A-H, 1-12) with corner location make the plate easy to use in a robotic sampler.
The 3 Lens plate is suitable for centrifugation.
The unique 3 drop protein crystallisation plate offers a new way of sitting-drop crystallography.
The 288 wells are optically perfect designed to observe crystals under a microscope.

3 Lens plate features
Easy to fill 96-lens structure – typically 30 microlitres per buffer lens.
Standard ANSI/SLAS 1-2004 Standard form for automation.
Optically perfect wells with lens effect for a better view with microscope.
Microscopic identifier within wells, simplifying the orientation under the microscope.
Maximum volume of the buffer reservoir is 40 microlitres, typically 10 nanolitres is used for the protein well filling.
Grown crystals are easy to identify and to remove from lens due to a low-binding polymer.
Plate with 3 lenses for each sample, better growing security with triplicates or the ability to use lens two and lens threeas mixing stations.
Lenses fill without micro-droplets jumping out due to static effects.
Wells can be individually sealed with a perfectly flat upper surface – there is a large land area designed to ensureintegrity of each lens section.
The profile allows for easier storage.
Low volume buffer well enables savings on reagents.
Triplicates allow for 288 constructs per plate.

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CAT NO

HR3-193

NAME

Swissci 3 Well Midi Crystallization Plate in UVXPO (10)

DESCRIPTION

10 plate case

PRICE

$97.00

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CAT NO

HR3-194

NAME

Swissci 3 Well Midi Crystallization Plate in UVXPO (40)

DESCRIPTION

40 plate case

PRICE

$375.00

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Support Material(s)

Swissci 3 Well Midi UVXPO (HR3-193/HR3-194) Plate Drawing

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Individual Slice pH Reagents蛋白结晶试剂盒Hampton Research

发表于2024年12月5日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Custom Shop Crystallization Reagents > Slice pH > Individual Slice pH Reagents

Individual Slice pH Reagents

Applications

Individual Slice pH reagents for screening, optimization and production

Features

185 ml volume
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-070-**
** Refers to the reagent number in the kit.
For example, Slice pH reagent number 1 = HR2-070-01

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Support Material(s)

HR2-070-** Individual Slice pH Reagents SDS

Polyethylene glycol 6,000蛋白结晶试剂盒Hampton Research

发表于2024年12月5日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 6,000

Polyethylene glycol 6,000

Applications

Crystallization grade Polyethylene glycol 6,000 for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Polyethylene glycol 6,000

Synonym: PEG 6,000
H(OCH₂CH₂)_nOH
M_r 5,000 – 7,000
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4105000
MDL Number MFCD00081839
PubChem Substance ID 24887715

HR2-533 Measured Conductivity range: 30.9 – 53.8 µS/cm at 25°C
HR2-533 Measured Refractive Index range: 1.40197 – 1.40251 at 20°C

DNases: none detected
RNases: none detected
Proteases: none detected
Phosphatases: none detected
Peroxides (as H₂O₂) < 0.001%

Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.02%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.02%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

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CAT NO

HR2-513

NAME

Polyethylene glycol 6,000

DESCRIPTION

500 grams

PRICE

$145.00

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CAT NO

HR2-533

NAME

50% w/v Polyethylene glycol 6,000

DESCRIPTION

200 mL

PRICE

$100.00

cart quote

Support Material(s)

HR2-513 Polyethylene glycol 6,000 (Flake) SDS

HR2-533 Polyethylene glycol 6,000 SDS

Certificate Of Analysis

References

1. McPherson, A. 1976, Crystallization of proteins from polyethylene glcyol. J. Biol. Chem. 251: 6300.

2. E144S active-site mutant of the bacillus cereus thermolysin-like neutral protease at 2.8 A resolution. Litster, SA; Wetmore, DR; Roche, RS; Codding, PW. Acta Crystallogr D 52, 543- 550, 1996.

3. Luft J.R. and DeTitta G.T. Chaperone salts, polyethylene glcyol and rates of equilibration in vapor-diffusion crystallization. Acta Crystallographica Section D 51: 780.

4. Gilliland, G.L. 1988. A biological macromolecule crystallization database: A basis for a crystallography strategy. J. Cryst. Growth. 90:51.

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Calcium chloride dihydrate蛋白结晶试剂盒Hampton Research

发表于2024年12月5日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Salts > Calcium chloride dihydrate

Calcium chloride dihydrate

Applications

Crystallization grade Calcium chloride dihydrate for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Calcium chloride dihydrate

Synonyms: None
CaCl₂• 2H₂O
Cl₂Ca • 2H₂O
M_r 147.01
CAS Number [10035-04-8]
EC Number 233-140-8
Merck 14,1659
RTECS EV9810000
MDL Number MFCD00149613
PubChem Substance ID 24852558
Purity ≥ 99.5%

Measured pH Range: 5.3 – 7.9 at 25°C
Measured Conductivity range: 170.0 – 198.8 mS/cm at 25°C
Measured Refractive Index range: 1.38108 – 1.38195 at 20°C

As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Cd ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.005%
Mn ≤0.0005%
Mo ≤0.0005%
N ≤0.002%
Na ≤0.01%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.01%
Zn ≤0.0005%

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CAT NO

HR2-557

NAME

2.0 M Calcium chloride dihydrate

DESCRIPTION

100 mL

PRICE

$92.00

cart quote

Support Material(s)

HR2-557 Calcium chloride dihydrate SDS

Certificate Of Analysis

References

1. The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 A and an analysis of the structural consequences of peroxide inactivation. Bott, R; Ultsch, M; Kossiakoff, A; Graycar, T; Katz, B; Power, S. J Biol Chem 263, 7895- 7906, 1988.

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Sodium phosphate monobasic monohydrate蛋白结晶试剂盒Hampton Research

发表于2024年12月4日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Salts > Sodium phosphate monobasic monohydrate

Sodium phosphate monobasic monohydrate

Applications

Crystallization grade Sodium phosphate monobasic monohydrate for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Sodium phosphate monobasic monohydrate

Synonyms: Monosodium phosphate or Sodium dihydrogen phosphate monohydrate
NaH₂PO₄ • H₂O
H₂NaO₄P • H₂O
M_r 137.99
CAS Number [10049-21-5]
EC Number 231-449-2
Merck 14,8660
MDL Number MFCD00149208
PubChem Substance ID 24886208
Purity ≥ 99.0%
pKa 6.8 – 7.2 (depending on the physicochemical characteristics during pKa determination)
pKa 7.2 at 20°C

Measured pH range: 3.4 – 3.5 at 25°C
Measured Conductivity Range: 58.8 – 60.5 mS/cm at 25°C
Measured Refractive Index Range: 1.38208 – 1.38243 at 20°C

As ≤0.00005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
Mn ≤0.0005%
N ≤0.001%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.003%
Zn ≤0.0005%

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CAT NO

HR2-551

NAME

4.0 M Sodium phosphate monobasic monohydrate

DESCRIPTION

200 mL

PRICE

$114.00

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Support Material(s)

HR2-551 Sodium phosphate monobasic monohydrate SDS

Sodium potassium phosphate dilution table

Certificate Of Analysis

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Individual GRAS Screen 7 Reagents蛋白结晶试剂盒Hampton Research

发表于2024年12月4日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Custom Shop Crystallization Reagents > GRAS Screen 7 > Individual GRAS Screen 7 Reagents

Individual GRAS Screen 7 Reagents

Applications

Individual GRAS Screen 7 reagents for screening, optimization, and production

Features

185 mL volume
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-457-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-457-01

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Support Material(s)

HR2-457-** Individual GRAS Screen 7 Reagents SDS

NDSB-211蛋白结晶试剂盒Hampton Research

发表于2024年12月4日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-211

NDSB-211

Applications

Crystallization grade NDSB-211 for formulating screens or for optimization

Features

Efficiently solubilizes proteins at non-denaturing concentrations
Useful in preventing protein aggregation
Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
Protein specific additive for solubility and stability

Description

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-211
Synonyms: Dimethyl(2-hydroxyethyl)ammonium propane sulfonate or Non detergent sulfobetaine 211
C₇H₁₇NO₄S
M_r 211.30
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

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CAT NO

HR2-793

NAME

NDSB-211

DESCRIPTION

5 grams

PRICE

$116.00

cart quote

Support Material(s)

HR2-793 NDSB-211 User Guide

HR2-793 NDSB-211 SDS

Certificate Of Analysis

References

1. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

2. Vuillard, L., et al. 1995. Biochem. J. 305-, 337.

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18 mm Siliconized Glass Cover Slides蛋白结晶试剂盒Hampton Research

发表于2024年12月3日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Crystallization Plates, Hardware & Accessories > Cover Slides & Related Tools > 18 mm Siliconized Glass Cover Slides

18 mm Siliconized Glass Cover Slides

Applications

Siliconized glass cover slides for hanging & sitting drop crystallization
Hanging drop crystallization
Sitting drop crystallization
Sandwich drop crystallization

Features

High purity of glass
Siliconized, hydrophobic glass surface
0.22 mm & 0.96 mm glass thickness
18 mm glass diameter
Circle
0.22 mm glass thickness is UV Compatible (>85% transmission)

Description

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×10¹² ohms Siliconized glass 1.2 x 10¹³ ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm²

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(+/-)-2-Methyl-2,4-pentanediol蛋白结晶试剂盒Hampton Research

发表于2024年12月3日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Organics (Non-Volatile) > (+/-)-2-Methyl-2,4-pentanediol

(+/-)-2-Methyl-2,4-pentanediol

Applications

Crystallization grade (+/-)-2-Methyl-2,4-pentanediol for formulating screens or for optimization

Description

(+/-)-2-Methyl-2,4-pentanediol

Synonyms: MPD or Hexylene glycol
C₆H₁₄O₂
CH₃CH(OH)CH₂C(CH₃)₂OH
M_r 118.18
CAS Number [107-41-5]
EC Number 203-489-0
Beilstein Registry Number 1098298
Merck 14,4710
RTECS SA0810000
MDL Number MFCD00004547
PubChem Substance ID 329760818
Purity ≥ 99.0%
Density 0.925 g/mL at 25°C(lit.)
Boiling point 197.0° C (lit.)
Vapor pressure 0.02 mm Hg at 20°C (lit.)

Measured Conductivity Range: 0.0 – 0.3 µS/cm at 25°C
Measured Refractive Index Range: 1.42725 – 1.42825 at 20°C

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CAT NO

HR2-627

NAME

100% (+/-)-2-Methyl-2,4-pentanediol

DESCRIPTION

200 mL

PRICE

$78.00

cart quote

Support Material(s)

HR2-627 (+/-)-2-Methyl-2,4-pentanediol SDS

Certificate Of Analysis

References

1. Crystallization and Preliminary X-ray Analysis of a Thermophillic Bacillus Xylanase. Pickersgill, RW; Debeire, P; Debeire-Gosselin, M; Jenkins, JA. J Mol Biol 230, 664- 666, 1993.

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StockOptions ADA Buffer Kit蛋白结晶试剂盒Hampton Research

发表于2024年12月2日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimization Screens > StockOptions Buffer Kits > StockOptions ADA Buffer Kit

StockOptions ADA Buffer Kit

Applications

Focused, titrated buffer stocks for crystal screening, optimization & production

Features

pH 5.6 to 7.5 in 0.1 pH unit increments (20 reagents)
1.0 M concentrated stocks
ADA pKa = 6.6

Description

StockOptions ADA buffer kit is a preformulated, sterile filtered set of titrated buffer stocks. The StockOptions buffer stock reagents are supplied as 1.0 M stock solutions in 10 milliliter volumes. Each StockOptions ADA buffer reagent is carefully titrated using Sodium hydroxide. StockOptions ADA is comprised of 20 unique reagents covering the pH range of 5.6 to 7.5 in 0.1 pH unit increments.

ADA (Buffer)
H₂NCOCH₂N(CH₂CO₂H) ₂ C₆H₁₀N₂O₅
M_r 190.16
CAS Number [26239-55-4]
EC Number 247-530-0
Merck 14,147
Beilstein Registry Number 1787181
Purity ≥99.0%
pKa = 6.6 at 25.0°C and 6.6 at 20.0°C
Useful pH range of ADA buffer is 5.6 – 7.5
MDL Number: MFCD00008031
PubChem Substance ID 24845005

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CAT NO

HR2-255

NAME

StockOptions ADA

DESCRIPTION

pH 5.6 to 7.5 in 0.1 pH unit increments

PRICE

$243.00

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Support Material(s)

HR2-255 StockOptions ADA Documents

HR2-255 StockOptions ADA SDS

Polyethylene glycol 600蛋白结晶试剂盒Hampton Research

发表于2024年12月2日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 600

Polyethylene glycol 600

Applications

Crystallization grade Polyethylene glycol 600 for formulating screens or for optimization

Features

HR2-859 100% Polyethylene glycol is a waxy solid at room temperature
HR2-860 80% w/v Polyethylene glycol is a solution at room temperature
HR2-860 is sterile filtered and formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Polyethylene glycol 600

Synonyms: PEG 600
H(OCH₂CH₂)_nOH
M_r 570-630
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24888888
Vapor density >1 (vs air)
Vapor pressure <0.01 mmHg (20°C)
Autoignition temperature 581°F
Refractive index (lit.) n20/D 1.468-1.470
Melting point 17-22°C
HR2-859 100% Polyethylene glycol is a waxy solid at room temperature
HR2-860 80% w/v Polyethylene glycol is a solution at room temperature
Solubility in water: soluble to 80% v/v at 25°C, clear, colorless
Density (100%) 1.13 g/mL at 20°C

HR2-859 Measured Refractive Index Range: 1.46874 – 1.46951 at 20°C
HR2-859 Measured Conductivity Range: 2.9 – 3.6 µS/cm at 25°C

HR2-860 Measured Refractive Index Range: 1.43676 – 1.46951 at 20°C
HR2-860 Measured Conductivity Range: 21.3 – 24.6 µS/cm at 25°C

Total impurities as insoluble matter: passes filter test
Peroxides (as H₂O₂) ≤0.001%
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.02%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.02%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

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CAT NO

HR2-859

NAME

100% Polyethylene glycol 600

DESCRIPTION

100 mL

PRICE

$44.00

cart quote

CAT NO

HR2-860

NAME

80% w/v Polyethylene glycol 600

DESCRIPTION

100 mL

PRICE

$36.00

cart quote

Support Material(s)

HR2-859 Polyethylene glycol 600 100% solution SDS

HR2-860 Polyethylene glycol 600 80% w/v solution SDS

Certificate Of Analysis

References

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Individual Heavy Atom Screen M1 Reagents蛋白结晶试剂盒Hampton Research

发表于2024年12月2日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

Individual Heavy Atom Screen M1 Reagents

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Products > Custom Shop Crystallization Reagents > Heavy Atom M1 > Individual Heavy Atom Screen M1 Reagents

Individual Heavy Atom Screen M1 Reagents

Applications

Individual Heavy Atom M1 screen reagents for heavy atom derivitization

Features

100 mg

Description

** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-448-01

Thallium (III) chloride hydrate and Thallium (I) chloride discontinued from HR2-448 Heavy Atom M1 kit effective lot 244816. Limited quantities available for individual reagent HR2-448-01A and HR2-448-02A.

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CAT NO

HR2-448-14

NAME

Individual GRAS Screen 3 Reagents蛋白结晶试剂盒Hampton Research

发表于2024年12月1日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Custom Shop Crystallization Reagents > GRAS Screen 3 > Individual GRAS Screen 3 Reagents

Individual GRAS Screen 3 Reagents

Applications

Individual GRAS Screen 3 reagents for screening, optimization, and production

Features

185 mL volume
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-453-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-453-01

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Support Material(s)

HR2-453-** Individual GRAS Screen 3 Reagents SDS

Grid Screen MPD

发表于2024年12月1日由Harlan Teklad中国

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Products > Crystallization Screens > Grid Screens > Grid Screen MPD

Grid Screen MPD

Applications

Primary or secondary, (+/-)-2-Methyl-2,4-pentanediol (MPD) versus pH grid crystallization screen for biological macromolecules

Features

A systematic grid screen varying (+/-)-2-Methyl-2,4-pentanediol (MPD) concentration versus pH
Samples pH 4 to 9
Samples four concentrations of (+/-)-2-Methyl-2,4-pentanediol (MPD)
Precise pH versus (+/-)-2-Methyl-2,4-pentanediol (MPD) screen
- pH adjusted after reagent addition; 25°Celsius
Combine reagents between rows or columns to create expanded grid screens

Description

Grid Screen™ MPD is a preformulated reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules.

The screen is simple and practical for finding initial crystallization conditions as well as determining the solubility of a macromolecule in (+/-)-2-Methyl-2,4-pentanediol (MPD) between pH 4.0 and 9.0.

Grid Screen MPD evaluates (+/-)-2-Methyl-2,4-pentanediol (MPD) at four concentrations (10, 20, 40, 65% v/v) versus six pH levels (4, 5, 6, 7, 8, 9).

Grid Screen MPD consists of 24 unique reagents, each supplied as 10 milliliter in sterile, screw cap tubes. Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity polyol and buffers. Individual reagents are available through the Hampton Research Custom Shop.

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CAT NO

HR2-215

NAME

Grid Screen MPD

DESCRIPTION

10 ml. tube format

PRICE

$126.00

cart quote

Support Material(s)

HR2-215 Grid Screen MPD Documents

HR2-215 Grid Screen MPD SDS

Grid Screen MPD Formulation & Scoring Data

References

1. A protein crystallization strategy using automated grid searches on successively finer grids. Patricia C. Weber. Methods: A Companion to Methods in Enzymology Vol. 1, No. 1, August, pp. 31-37, 1990.

2. Crystallization of Biological Macromolecules. Alexander McPherson. pp. 271-329. Cold Spring Harbor Laboratory Press, 1999. ISBN 0-87969-527-7.

3. Advance in Protein Chemistry Volume 41. Pages 1-33 (Patricia C. Weber). Academic Press, 1991.

4. Crystallization of nucleic acids and proteins, Edited by A. Ducruix and R. Giege, The Practical Approach Series, Oxford Univ. Press, 1992.

5. Current approaches to macromolecular crystallization. McPherson, A. Eur. J. Biochem. 189, 1-23, 1990.

6. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

7. Crystallization studies of the keratin-like domain from Arabidopsis thaliana SEPALLATA 3. Samira Acajjaoui and Chloe Zubieta. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep 1; 69(Pt 9): 997-1000.

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VDXm Plate with sealant蛋白结晶试剂盒Hampton Research

发表于2024年11月30日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Crystallization Plates, Hardware & Accessories > 24 Well Crystallization Plates > VDXm Plate with sealant

VDXm Plate with sealant

Applications

Hanging drop crystallization

Features

24 well plate with raised cover
Sealant applied to 24 wells, ready to seal with cover slides
Compatible with 18 mm diameter circle cover slides
Optically clear plastic
128 mm x 85 mm microplate footprint
Compatible with UV crystal imaging when used with HR3-239 and HR3-241 – 18 mm x 0.22 mm Siliconized circle cover slides

Description

24 well plate with sealant for hanging drop vapor diffusion crystallization with a 128 mm x 85 mm microplate footprint. Stackable, optically clear plastic, 24 well plates with raised covers (to allow room for cover slides) and flat bottoms for exceptional optics. Raised, wide rings around each reservoir (well) minimize cross contamination and allow each well to be individually sealed with 18 mm diameter circle cover slides. Plates are individually wrapped and supplied with applied sealant.

The microplate footprint is space-saving and compatible with automated liquid handling systems.

Approximate dimensions: 128 mm x 85 mm.
Approximate well ID: 14.4 mm.
Typical well volume: 100 to 500 µl.
Height with cover 19.5 mm.
Height without cover with 18 x 0.22 mm cover slide 16 mm.
Height without cover with 18 x 0.96 mm cover slides 17 mm.

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CAT NO

HR3-306

NAME

VDXm™ Plate with sealant

DESCRIPTION

50 plate case

PRICE

$270.00

cart quote

References

1. Expression and crystallization of DsbA from Staphylococcus aureus. J.L. Martin et al. Acta Cryst. (2007). F63, 953-956.

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Parabar 10312 (previously known as Paratone)蛋白结晶试剂盒Hampton Research

发表于2024年11月29日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

Parabar 10312 (previously known as Paratone)

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Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)

Parabar 10312 (previously known as Paratone)

Applications

Oil based cryoprotectant

Features

Synonyms used in the crystallographic literature: Paratone^® N, Paratone^® 8277, Infineum V8512
Oil based cryoprotectant

Description

Sorry, Parabar 10312 is no longer available. We’ve left this page up for reference only. See HR2-861 Santovac Cryo Oil as a possible substitute.

Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.¹

Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.

HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.

HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.

The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.

Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.

Refractive index 1.48487 at 20°C.

Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.

Clear to slightly hazy is appearance.

Synonyms used in the crystallographic literature: Paratone^® N, Paratone^® 8277, Infineum V8512.

Paratone^® is a registered trademark of Chevron Corporation.

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Support Material(s)

HR2-862 Parabar 10312 User Guide

HR2-643 HR2-862 Parabar 10312 SDS

Certificate Of Analysis

References

1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.

2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.

3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.

4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.

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Harlan Teklad动物饲料Vision, mission and values

发表于2024年11月29日由Harlan Teklad中国

上海金畔生物科技有限公司代理Harlan Teklad动物饲料全线产品，欢迎访问Harlan Teklad动物饲料官网了解更多信息。

Vision, mission and values

Vision

Working together to build a healthier and safer world

Mission

To be the best company to work with and the best company to work for

Values

Doing our best work, together, every day
Caring about each other, our animals and the environment
Being honest and respectful
Always learning, challenging and improving

Polyethylene glycol 3,000蛋白结晶试剂盒Hampton Research

发表于2024年11月29日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 3,000

Polyethylene glycol 3,000

Applications

Crystallization grade Polyethylene glycol 3,000 for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Polyethylene glycol 3,000

Synonyms: PEG 3,000
H(OCH₂CH₂)_nOH
M_r 2,700-3,300
CAS Number [25322-68-3]
EC Number 500-038-2
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24887699

Measured Conductivity Range: 44.8 – 45.4 µS/cm at 25°C
Measured Refractive Index Range: 1.40163 – 1.40173 at 20°C

Peroxides (as H₂O₂) ≤0.001%

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CAT NO

HR2-604

NAME

50% w/v Polyethylene glycol 3,000

DESCRIPTION

200 mL

PRICE

$100.00

cart quote

Support Material(s)

HR2-604 Polyethylene glycol 3,000 SDS

Certificate Of Analysis

References

1. Discovery of novel bacterial queuine salvage enzymes and pathways in human pathogens. Yuan, Y., Zallot, R., Grove, T.L., Payan, D.J., Martin-Verstraete, I., Sepic, S., Balamkundu, S., Neelakandan, R., Gadi, V.K., Liu, C.F., Swairjo, M.A., Dedon, P.C., Almo, S.C., Gerlt, J.A., de Crecy-Lagard, V. (2019) Proc Natl Acad Sci U S A 116: 19126-19135 PubMed: 31481610 DOI: 10.1073/pnas.1909604116

2. Crystal structure of the N-terminal domain of TagH reveals a potential drug targeting site. Yang, C.S., Huang, W.C., Ko, T.P., Wang, Y.C., Wang, A.H., Chen, Y. (2020) Biochem Biophys Res Commun 536: 1-6 PubMed: 33360015 DOI: 10.1016/j.bbrc.2020.12.028

3. Intracellular Neutralization of Ricin Toxin by Single-domain Antibodies Targeting the Active Site. Rudolph, M.J., Czajka, T.F., Davis, S.A., Thi Nguyen, C.M., Li, X.P., Tumer, N.E., Vance, D.J., Mantis, N.J. (2020) J Mol Biol 432: 1109-1125 PubMed: 31931008 DOI: 10.1016/j.jmb.2020.01.006

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Quartz Capillaries蛋白结晶试剂盒Hampton Research

发表于2024年11月26日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Capillaries, Mounts & Supplies > Capillaries > Quartz Capillaries

Quartz Capillaries

Applications

X-ray data collection
Liquid-liquid diffusion crystallization
Gel acupuncture crystallization

Features

Thin walled – 10 micron
Quartz Glass 0620

Description

Crystal Clear Quartz capillaries that are extremely thin walled (approximately 10 micron wall thickness). The length of the capillary has a well defined diameter, with one end having a funnel shape and the other end closed. Quartz capillaries have a wall thickness of 0.01 mm and an overall length of 80 mm +/-5 mm. Quartz capillaries are available in a wide range of outside diameters from 0.1 mm to 5.0 mm. They are designed to mount, hold, and store small molecule and biological macromolecular crystals for x-ray data collection. Capillaries can also be used for crystal density measurements and crystal growth experiments. The capillaries can be sealed tightly against moisture and gases using wax, epoxy, or other sealing materials.

In determining what glass or quartz capillary is right for you, please refer to the “Linear Absorption Coefficient µ cm-1” table. This table indicates the amount of radiation that is absorbed by the capillary during x-ray data collection.

For 0.1 mm to 2.5 mm capillaries the open end capillary tube base size is 3.0 +/- 0.2 mm OD x 0.2 +/- 0.15 mm Wall thickness. For 3.0 and 3.5 mm capillaries the open end capillary base size is about 4.4 mm OD x 0.25 mm Wall thickness. For 4.0 and 5.0 mm capillaries the open end capillary based size is about 6.0 x 0.25 mm.

The Diameter is measured about 30 to 40 mm from the closed end (measuring instrument: LaserMicrometer LS 7500) The tolerances are as follows.

Diameter Tolerance Minimum Diameter Maximum Diameter

0.1 mm       +/-0.05 mm          0.05 mm                     0.15 mm
0.2 mm       +/-0.05 mm          0.15 mm                     0.25 mm
0.3 mm       +/-0.05 mm          0.25 mm                     0.35 mm
0.4 mm       +/-0.05 mm      0.35 mm                     0.45 mm
0.5 mm       +/-0.05 mm          0.45 mm                     0.55 mm
0.6 mm       +/-0.05 mm          0.55 mm                     0.65 mm
0.7 mm       +/-0.05 mm      0.65 mm                     0.75 mm
0.8 mm       +/-0.05 mm          0.75 mm                     0.85 mm
0.9 mm       +/-0.05 mm          0.85 mm                     0.95 mm
1.0 mm       -0.05 +0.25 mm    0.95 mm                     1.25 mm
1.5 mm       +/-0.25 mm          1.25 mm                     1.75 mm
2.0 mm       +/-0.25 mm          1.75 mm                     2.25 mm
2.5 mm       +/-0.05 mm          2.25 mm                     2.55 mm
3.0 mm +/-0.25 mm          2.75 mm                     3.25 mm
3.5 mm       +/-0.25 mm          3.25 mm                     3.75 mm
4.0 mm       +/-0.25 mm          3.75 mm                     4.25 mm
5.0 mm       +/-0.25 mm          4.75 mm                     5.25 mm

Quartz Specifications
Glass type Fused silica / quartz glass
Density 2.2 g/cm³
Transformation temperature Tg= 1075 – 1210 degrees Celsius
Strain point approximately 1075 degrees Celsius
Annealing point approximately 1180 degrees Celsius
Softening point approximately 1730 degrees Celsius
Working point 1700 – 2100 degrees Celsius
Refractive index 1.46 589.3nm
Mohs hardness 5.5 – 6.5
Hydrolytic resistance, class 1 (DIN 12 111)
Acid resistance, class 1 (DIN 12 116)
Alkalai resistance, class 1 (DIN 52 322)
Chemical composition SiO₂ 99.99%
Trace elements
Al 16.00 ppm
Ca 0.80 ppm
Fe 0.80 ppm
K 0.90 ppm
Li 0.70 ppm
Na 0.90 ppm
Ti 1.50 ppm
Mg 0.10 ppm
Cu <0.05 ppm
Cr <0.05 ppm

Capillaries have only been tested at atmospheric pressure (760 mmHg (torr), 29.92 inHg, 14.696 psi). Use at other pressures has not been tested.

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Support Material(s)

Tolerances for Capillaries

Quartz Technical Data Sheet

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Duco Cement蛋白结晶试剂盒Hampton Research

发表于2024年11月25日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Capillaries, Mounts & Supplies > Capillary Sealants > Duco Cement

Duco Cement

Applications

Sealing capillaries
Mount crystals to glass fibers

Description

Old school cement for sealing glass capillaries when mounting crystals. Easy to apply. Requires no heat. Fast-drying, medium-strength cement dries clear. Bonds glass, wood, some plastics, and metal. Not recommended for most polyethylene or polypropylene plastics. Sets in 5 minutes, item can be handled in 1 hour, full bond strength in 16 hours. 1 ounce (29.5 ml) tube.

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CAT NO

HR4-320

NAME

Duco Cement

DESCRIPTION

29.5 ml tube

PRICE

$8.00

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Support Material(s)

HR4-320 Duco Cement SDS

Individual Proti-Ace & Proti-Ace 2 Reagents蛋白结晶试剂盒Hampton Research

发表于2024年11月24日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

Individual Proti-Ace & Proti-Ace 2 Reagents

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Products > Custom Shop Crystallization Reagents > Proti-Ace & Proti-Ace 2 > Individual Proti-Ace & Proti-Ace 2 Reagents

Individual Proti-Ace & Proti-Ace 2 Reagents

Applications

In situ proteolysis and proteolytic screening of protein samples for crystallization and structure determination
Individual reagents from Proti-Ace & Proti-Ace 2 kits

Features

Lyophilized enzymes for enhanced stability

Description

Individual reagents from the Proti-Ace and Proti-Ace 2 kits. Each protease is supplied in a stable, lyophilized format in an optimized digest buffer. Simply add water when ready to use.

The unique freeze dried formulation of the Proti-Ace kit offers a much improved protease stability compared to liquid protease formulations. Each tube contains 0.1 mg of lyophilized enzyme and digest buffer as shown below. Add 100 microliters of deionized water (Type 1+) to each tube of lyophilized content to create a 1 mg/ml protease solution in digest buffer. Proti-Ace Dilution buffer is supplied as a ready to use solution.

Proti-Ace Reagent 1* (Qty 3):
• 1 mg/ml a-Chymotrypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 2* (Qty 3):
• 1 mg/ml Trypsin, 1 mM Hydrochloric acid, 2 mM Calcium chloride dihydrate
Proti-Ace Reagent 3* (Qty 3):
• 1 mg/ml Elastase, 200 mM Tris pH 8.0
Proti-Ace Reagent 4* (Qty 3):
• 1 mg/ml Papain, 2 mM EDTA, 2 mM TCEP hydrochloride, 2 mM L-Cysteine
Proti-Ace Reagent 5* (Qty 3):
• 1 mg/ml Subtilisin, 10 mM Sodium acetate trihydrate pH 7.5, 5 mM Calcium acetate hydrate
Proti-Ace Reagent 6* (Qty 3):
• 1 mg/ml Endoproteinase Glu-C (in deionized water)
* When 100 µl of deionized water is added to each Proti-Ace freeze dried reagent

Proti-Ace Dilution Buffer (Qty 3):
• 1.6 ml of 10 mM HEPES pH 7.5, 500 mM Sodium chloride

Proti-Ace 2 Reagent 1* (Qty 3):
• 1 mg/ml Proteinase K, 10 mM TRIS hydrochloride pH 7.5, 1 mM Calcium acetate hydrate
Proti-Ace 2 Reagent 2* (Qty 3):
• 1 mg/ml Clostripain (Endoproteinase-Arg-C), 10 mM HEPES pH 7.5, 2 mM TCEP hydrochloride, 2 mM L-Cysteine, 1 mM Calcium chloride dihydrate
Proti-Ace 2 Reagent 3* (Qty 3):
• 1 mg/ml Pepsin, 1 mM Hydrochloric acid
Proti-Ace 2 Reagent 4* (Qty 3):
• 1 mg/ml Thermolysin, 10 mM Calcium acetate hydrate pH 8.0, 50 mM Sodium chloride
Proti-Ace 2 Reagent 5* (Qty 3):
• 1 mg/ml Bromelain, 5 mM HEPES pH 7.5, 2 mM L-Cysteine, 1 mM Ethylenediaminetetraacetic acid disodium salt dihydrate
Proti-Ace 2 Reagent 6* (Qty 3):
• 1 mg/ml Actinase, 5 mM HEPES pH 7.5, 10 mM Sodium chloride
* When 100 µl of deionized water is added to each Proti-Ace 2 freeze dried reagent

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Support Material(s)

HR2-429-** Individual Proti-Ace Reagents SDS

HR2-432-** Individual Proti-Ace 2 Reagents SDS

Sodium malonate蛋白结晶试剂盒Hampton Research

发表于2024年11月24日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Salts > Sodium malonate

Sodium malonate

Applications

Crystallization grade Sodium malonate for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

The Sodium malonate offered by Hampton Research is Malonic acid (CAS Number 141-82-2) titrated to pH 4.0-8.0 using Sodium hydroxide as described in the publication “A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.”. In this same publication, Sodium malonate was reported to be more successful than any other salt, resulting in the crystallization of 19 of the 23 macromolecules, almost twice as effective as the next most successful salt, which was a draw between sodium acetate, sodium tartrate, sodium formate, and ammonium sulfate. The high success rate of Sodium malonate in producing crystals was even more impressive when an overall unique success rate with individual macromolecules was considered.

The use of Sodium malonate in place of Ammonium sulfate has been reported in one instance to improve the reproducibility of crystals and improve resistance to physicochemical shocks. Sodium malonate also acted as a good cryoprotectant (Xing and Xu 2003).

One may combine the four different sodium malonate stocks to create a pH versus sodium malonate concentration grid for screening or optimization. Refer to the pH/Dilution Table for Sodium malonate.

Based on the structure of malonic acid titrated with sodium hydroxide, there are no chiral carbons so it is not characterized as having enantiomeric structures, such as D or L.

Synonyms: Propanedioic acid or Malonic acid
C₃H₄O₄ (before titration with NaOH)
M_r 104.06 (before titration with NaOH)
CAS Number 141-82-2
EC Number 205-503-0
Beilstein Registry Number 1751370
Merck 14,5710
RTECS OO0175000
MDL Number MFCD00002707
Purity > 99.0%
pKa1 2.8
pKa2 5.7

HR2-747 Measured Conductivity Range: 73.3 – 75.3 mS/cm at 25°C
HR2-747 Measured Refractive Index Range: 1.38365 – 1.38425 at 20°C

HR2-749 Measured Conductivity Range: 77.2 – 78.0 mS/cm at 25°C
HR2-749 Measured Refractive Index Range: 1.39264 – 1.39300 at 20°C

HR2-751 Measured Conductivity Range: 73.4 – 75.2 mS/cm at 25°C
HR2-751 Measured Refractive Index Range: 1.39938 – 1.39973 at 20°C

HR2-707 Measured Conductivity Range: 71.0 – 73.7 mS/cm at 25°C
HR2-707 Measured Refractive Index Range: 1.40090 – 1.40098 at 20°C

HR2-807 Measured Conductivity Range: 71.8 – 75.0 mS/cm at 25°C
HR2-807 Measured Refractive Index Range: 1.40106 – 1.40115 at 20°C

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Support Material(s)

HR2-747 Sodium malonate pH 4.0 SDS

HR2-749 Sodium malonate pH 5.0 SDS

HR2-751 Sodium malonate pH 6.0 SDS

HR2-707 Sodium malonate pH 7.0 SDS

HR2-807 Sodium malonate pH 8.0 SDS

pH/Dilution Table for Sodium Malonate

Certificate Of Analysis

References

1. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

2. Crystallization of the PX domain of cytokine-independent survival kinase (CISK): improvement of crystal quality for X-ray diffraction with sodium malonate. Yi Xing and Wenqing Xu. Acta Cryst. (2003). D59, 1816-1818.

3. Malonate: a versatile cryoprotectant and stabilizing solution for salt-grown macromolecular crystals. T. Holyoak, T. D. Fenn, M. A. Wilson, A. G. Moulin, D. Ringe and G. A. Petsko. Acta Cryst. (2003). D59, 2356-2358.

4. Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase. Kim et al Acta Cryst. (2007). F63, 579–581.

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Harlan Teklad动物饲料Doxycycline diets

发表于2024年11月23日由Harlan Teklad中国

上海金畔生物科技有限公司代理Harlan Teklad动物饲料全线产品，欢迎访问Harlan Teklad动物饲料官网了解更多信息。

Doxycycline diets

Envigo Teklad diets makes a variety of doxycycline containing diets for your Tet regulated systems. TD.01306 with doxycycline hyclate added at 625 mg/kg to Teklad global 2018 rodent diet is stocked and can typically ship within 1-2 days (non-irradiated) or 1-2 weeks (irradiated). Diets are easily customizable. Custom specifications include: base diet, concentration of doxycycline, and food coloring. Contact us to discuss your needs if you don’t find a suitable option below.

See our lists of doxycycline references and phytoestrogen references for additional information.

Formula examples with Teklad global 2018 base diet

Doxycycline hyclate¹ USP (mg/kg diet)	Natural color	Alternatives with food coloring
46	TD.10483	TD.180327 Red TD.120240 Blue
100	TD.04233	TD.130840 Blue
200	TD.00502	TD.180625 Red TD.150431 Blue TD.04104 Green
625	TD.01306 stocked²	TD.08541 Red TD.120769 Blue TD.09628 Green
1000	TD.05298	TD.06294 Red TD.120658 Blue
2000	TD.05512	TD.140011 Red TD.09633 Green
6000	TD.01533

¹ Doxycycline hyclate is ~87% doxycycline
² TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation

Considerations when choosing a base diet

Teklad Global Rodent Diets^® like 2018 do not contain alfalfa and are thus ideal if your work requires an alfalfa-free diet for fluorescent imaging, If further imaging clarity is needed, we can make purified diets with doxycycline.
Teklad global 2018 (base diet in examples above) contains a moderate amount of soybean meal, a source of phytoestrogens. The table below includes several options of minimal phytoestrogen diets with the most common doxycycline concentration of 625 mg/kg diet. For other concentrations contact us. For additional phytoestrogen information, see our list of phytoestrogen references.

Examples of minimal phytoestrogen global rodent diets with doxycycline hyclate¹ at 625 mg/kg diet

Base diet	Natural color	Alternatives with food coloring mg per kg diet
2014	TD.00426	TD.07382 Red TD.07573 Yellow
2016	TD.02503	TD.140263 Blue TD.170364v Red
2019	TD.09651	TD.09761 Green
2020	TD.110720	TD.130141 Red

¹ Doxycycline hyclate is ~87% doxycycline

Advantages of diet delivery of doxycycline

Typically the same doxycycline concentration can be used in diet as in water. Lower concentrations of doxycycline may be adequate with 625 mg/kg diet being the most common concentration.
Diet offers protection from light. Water may have to be given in dark-colored or foil-wrapped bottles
Diet is typically changed once per week. Water may need to be changed every few days
Diet delivery may also reduce risk of dehydration and preclude the need to give with sucrose (Cawthorne et. al. 2007)

Key planning information

Minimum order quantity is 3 kg, sufficient for feeding ~20 mice for one month
Store diet refrigerated and plan to use within six months
Typical lead time is two weeks (four weeks if irradiated)
TD.01306 typically ships within 2 days non-irradiated or 2 weeks with irradiation
Irradiation (20 – 50 kGy) is optional, and must be requested at time of order

Safe handling of doxycycline diets

Doxycycline is a tetracycline class antibiotic.
Usual dose for therapeutic effects in humans is 100-200 mg/day.
Doxycycline diets for rodents typically contain ~2 mg/pellet.
Accidental doxycycline exposure can be minimized by using typical lab precautions of lab coat, gloves, and mask when handling the diet.
Your chemical safety department should be contacted for additional institution specific guidelines for handling and disposal of doxycycline containing diets.

See our list of doxycycline references for additional information.

Individual Heavy Atom Pt Reagents蛋白结晶试剂盒Hampton Research

发表于2024年11月23日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Custom Shop Crystallization Reagents > Heavy Atom Pt > Individual Heavy Atom Pt Reagents

Individual Heavy Atom Pt Reagents

Applications

Individual Heavy Atom Pt screen reagents for heavy atom derivitization

Features

100 mg

Description

** Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-442-01

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Support Material(s)

HR2-442-** Individual Heavy Atom Screen Pt Reagents SDS

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Polyethylene glycol 400蛋白结晶试剂盒Hampton Research

发表于2024年11月22日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 400

Polyethylene glycol 400

Applications

Crystallization grade Polyethylene glycol 400 for formulating screens or for optimization

Description

Polyethylene glycol 400

Synonyms: PEG 400
H(OCH₂CH₂)_nOH
M_r 380-420
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3675000
MDL Number MFCD00081839
PubChem Substance ID 24897878
Viscosity (Rotation) 105 – 152 mPas
Density 1.126 g/mL at 20°C

Measured Conductivity Range: 2.3 – 3.4 µS/cm at 25°C
Measured Refractive Index Range: 1.46629 – 1.46762 at 20°C

Aldehydes None detected
Peroxides (as H₂O₂) ≤0.001%
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.2%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.2%
Ni ≤0.0005%
Pb ≤0.0005%
SO₄ ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%

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CAT NO

HR2-603

NAME

100% Polyethylene glycol 400

DESCRIPTION

200 mL

PRICE

$67.00

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Support Material(s)

HR2-603 Polyethylene glycol 400 SDS

Certificate Of Analysis

References

1. Crystallographic study of the large tryptic fragments of elongation factor G from Escherichia coli. Reshetnikova, LS; Garber, MB; Fomenkova, NP; Nikonov, SV; Chirgadze, YN. J Mol Biol 160, 127- 132, 1982.

2. Rapid crystallization of chemically synthesized hammerhead RNAs using a double screening procedure. Scott, WG; Finch, JT; Grenfell, R; Fogg, J; Smith, T; Gait, MJ; Klug, A. J Mol Biol 250, 327- 332, 1995.

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Magnesium sulfate hydrate蛋白结晶试剂盒Hampton Research

发表于2024年11月22日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Salts > Magnesium sulfate hydrate

Magnesium sulfate hydrate

Applications

Crystallization grade Magnesium sulfate hydrate for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

Magnesium sulfate hydrate

Synonyms: Magnesium sulphate hydrate
MgSO₄ • aqueous
M_r 120.37 • aqueous
CAS Number [22189-08-8]
EC Number 231-298-2
MDL Number MFCD00011110
PubChem Substance ID 24882418
Purity ≥ 99.0%

Measured pH Range: 7.8 – 8.3 at 25°C
Measured Conductivity Range: 57.9 – 59.1 mS/cm at 25°C
Measured Refractive Index Range: 1.36702 – 1.37174 at 20°C

Ca ≤0.02%
Cd ≤0.005%
Cl ≤0.01%
Co ≤0.005%
Cu ≤0.005%
Fe ≤0.005%
K ≤0.05%
Na ≤0.01%
Ni ≤0.005%
Pb ≤0.005%
Zn ≤0.005%

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CAT NO

HR2-633

NAME

2.5 M Magnesium sulfate hydrate

DESCRIPTION

200 mL

PRICE

$103.00

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Support Material(s)

HR2-633 Magnesium sulfate hydrate SDS

Certificate Of Analysis

References

1. Rudikoff, S; Potter, M; Segal, DM; Padlan, EA; Davies, DR; Proc Natl Acad Sci U S A 69, 3689- 3692, 1972.

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Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents蛋白结晶试剂盒Hampton Research

发表于2024年11月22日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents

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Products > Custom Shop Crystallization Reagents > Crystal Screen • Crystal Screen 2 > Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents

Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents

Applications

Individual reagents from Crystal Screen • Crystal Screen 2 • Crystal Screen HT for screening, optimization and production

Features

185 ml volume
Sterile filtered
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

HR2-910-** or HR2-912-**

** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-910-01

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Support Material(s)

HR2-910-** Individual Crystal Screen Reagents SDS

HR2-912-** Individual Crystal Screen 2 Reagents SDS

Tacsimate pH 4, 5, 6, 7, 8, & 9蛋白结晶试剂盒Hampton Research

发表于2024年11月21日由Harlan Teklad中国

Hampton Research蛋白结晶试剂盒

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Products > Optimize Reagents > Optimize – Salts > Tacsimate pH 4, 5, 6, 7, 8, & 9

Tacsimate pH 4, 5, 6, 7, 8, & 9

Applications

Crystallization grade Tacsimate for formulating screens or for optimization

Features

Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)

Description

To screen pH as a crystallization variable using Tacsimate one can mix together different ratios of different pH Tacsimate solutions. Refer to the pdf “Mixing Tacsimate Stocks to Screen pH” on this page. Or, one can add a buffer to the crystallizaiton reagent containing Tacsimate. Recommended final buffer concentration in the reagent well is 0.05 to 0.1 M.

Tacsimate
A pH titrated mixture of organic acids. 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic. pH adjusted using NaOH

Measured pH of 100% Tacsimate is 4.0 at 25°C
Measured pH of 100% Tacsimate is 5.0 at 25°C
Measured pH of 100% Tacsimate is 6.0 at 25°C
Measured pH of 100% Tacsimate is 7.0 at 25°C
Measured pH of 100% Tacsimate is 8.0 at 25°C
Measured pH of 100% Tacsimate is 9.0 at 25°C

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Support Material(s)

What is Tacsimate?

Mixing Tacsimate Stocks to Screen Ph

HR2-823 Tacsimate 100% solution pH 4.0 SDS

HR2-825 Tacsimate 100% solution pH 5.0 SDS

HR2-827 Tacsimate 100% solution pH 6.0 SDS

HR2-755 Tacsimate 100% solution pH 7.0 SDS

HR2-829 Tacsimate 100% solution pH 8.0 SDS

HR2-813 Tacsimate 100% solution pH 9.0 SDS

Certificate Of Analysis

References

1. Searching for silver bullets: An alternative strategy for crystallizing macromolecules. Alexander McPherson and Bob Cudney. Journal of Structural Biology 156 (2006) 387–406.

2. A novel strategy for the crystallization of proteins: X-ray diffraction validation. Steven B. Larson, John S. Day, Robert Cudney and Alexander McPherson. Acta Cryst. (2007). D63, 310–318.

3. Structure of the catalytic and ubiquitin-associated domains of the protein kinase MARK/Par-1. Eckhard Mandelkow et al. Structure 14, 173-183, February 2006.

4. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

5. Crystallization of a newly discovered histidine acid phosphatase from Francisella tularensis. Richard L. Felts, Thomas J. Reilly, Michael J. Calcutt, and John J. Tanner. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 January 1; 62(Pt 1): 32–35.

6. Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila. Petermann et al, FEBS Letters Volume 584, Issue 4, Pages 733-738 (19 February 2010).

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