Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > StockOptions Tricine > Individual StockOptions Tricine Reagents
Individual StockOptions Tricine Reagents
Applications
- Individual StockOptions Tricine reagents for screening, optimization and production
Features
- 1.0 M concentrated stocks
- 0.1 unit increments for fine tuning pH
- Available in pH 7.4 to 8.8
- 185 ml volume
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-253-**
** Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-253-01
Synonym: N-[Tris(hydroxymethyl)methyl]glycine
(HOCH2)3CNHCH2CO2H
Mr 179.17
CAS Number [5704-04-1]
Beilstein Registry Number 1937804
EC Number 227-193-6
MDL Number MFCD00004277
PubChem Substance ID 24889851
Purity ≥ 99.5%
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CAT NO
HR2-253-01
NAME
DESCRIPTION
1.0 M Tricine pH 7.4
PRICE
$181.00
cart quote
CAT NO
HR2-253-02
NAME
DESCRIPTION
1.0 M Tricine pH 7.5
PRICE
$181.00
cart quote
CAT NO
HR2-253-03
NAME
DESCRIPTION
1.0 M Tricine pH 7.6
PRICE
$181.00
cart quote
CAT NO
HR2-253-04
NAME
DESCRIPTION
1.0 M Tricine pH 7.7
PRICE
$181.00
cart quote
CAT NO
HR2-253-05
NAME
DESCRIPTION
1.0 M Tricine pH 7.8
PRICE
$181.00
cart quote
CAT NO
HR2-253-06
NAME
DESCRIPTION
1.0 M Tricine pH 7.9
PRICE
$181.00
cart quote
CAT NO
HR2-253-07
NAME
DESCRIPTION
1.0 M Tricine pH 8.0
PRICE
$181.00
cart quote
CAT NO
HR2-253-08
NAME
DESCRIPTION
1.0 M Tricine pH 8.1
PRICE
$181.00
cart quote
CAT NO
HR2-253-09
NAME
DESCRIPTION
1.0 M Tricine pH 8.2
PRICE
$181.00
cart quote
CAT NO
HR2-253-10
NAME
DESCRIPTION
1.0 M Tricine pH 8.3
PRICE
$181.00
cart quote
CAT NO
HR2-253-11
NAME
DESCRIPTION
1.0 M Tricine pH 8.4
PRICE
$181.00
cart quote
CAT NO
HR2-253-12
NAME
DESCRIPTION
1.0 M Tricine pH 8.5
PRICE
$181.00
cart quote
CAT NO
HR2-253-13
NAME
DESCRIPTION
1.0 M Tricine pH 8.6
PRICE
$181.00
cart quote
CAT NO
HR2-253-14
NAME
DESCRIPTION
1.0 M Tricine pH 8.7
PRICE
$181.00
cart quote
CAT NO
HR2-253-15
NAME
DESCRIPTION
1.0 M Tricine pH 8.8
PRICE
$181.00
Epoxy蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Cryocrystallography > CryoLoops > Epoxy
Epoxy
Applications
- Sealing capillary tubes
- Securing MicroTubes into CrystalCaps
- Securing CryoLoops into MicroTubes
Features
- Fast setting
- Excellent cohesive strength
Description
Double/Bubble® Epoxy HR4-347 extra fast setting with a work time of 3 to 5 minutes. The preferred epoxy for cryocrystallography. Sold as a pack of 5. Hardman Double/Bubble Fast-Setting Epoxy is an extra fast setting, two part, room temperature curing epoxy adhesive. It has a convenient mix ratio, excellent cohesive strength and bonds to most substrates including metal, wood, concrete, ceramics and many plastics. The Double/Bubble package is a handy, dual-pouch, one-shot, job-size packaging concept. Fills gaps, will not shrink. Contains no solvents. Two part epoxy in a single, dual-pouch 3.5 gram packet.
To use:
1. Fold Double/Bubble packet
2. Cut or tear to open end
3. Squeeze out adhesive
4. Mix parts A and B together with supplied spatula
Supplied as 5 packets with 5 spatulas.
Color/Appearance: Amber
Product Name: Hardman DOUBLE/BUBBLE Extra-Fast Set Epoxy Red Package 3.5 g Packet 1 part or 2: 2 part
Base Chemistry: Part A: Epoxy; Part B: Polymer
Handling strength: 15 to 30 minutes at 25°C
Cure Time: 24h at 25°C
Durometer/Hardness: 80 D at 25°C
Flash Point: Part A: 190.5°C; Part B: 132.2°C
Mix Ratio: 1:1
Peel Strength: 1.8 pli at 25°C
Service Temperature: Up to 82°C
Shear Strength Psi: 3,000 at 25°C
Specific Gravity: Mixed: 1.16 at 25°C
Viscosity Cp: Mixed: 40,000 at 25°C
Work Life/Pot Life: 3 minutes
Click to Zoom In
CAT NO
HR4-347
NAME
DESCRIPTION
5 pack
PRICE
$14.00
cart quote
Support Material(s)
HR4-347 Double/Bubble Epoxy SDSRelated Item(S)
- 18 mm Mounted CryoLoop™ – 20 micron
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Harlan Teklad动物饲料2020X Teklad global soy protein-free extruded rodent diets
上海金畔生物科技有限公司代理Harlan Teklad动物饲料全线产品，欢迎访问Harlan Teklad动物饲料官网了解更多信息。
2020X Teklad global soy protein-free extruded rodent diets
Product Features
- Designed to support gestation, lactation, and growth of rodents
- Formulated to exclude soybean meal, thus minimizing the presence of isoflavones, the primary type of phytoestrogen found in lab animal diets
- Typical isoflavone concentrations (daidzein + genistein aglycone equivalents) range from non-detectable to 20 mg/kg
- Exclusion of alfalfa reduces chlorophyll, greatly improving fluorescent optical imaging clarity
- Absence of animal protein and fish meal minimizes the presence of nitrosamines (a potential carcinogen)
Products
Not all products are stocked locally; extended lead time and additional fees may apply.
Many diets are available in certified format designated by a “C” following the product code. When diets are certified a representative sample is tested for a panel of contaminants. If not stocked as certified, certification can be made available upon request. Minimum order size and additional charges may apply.
BIS-TRIS Buffer蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer
BIS-TRIS Buffer
Applications
- Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
BIS-TRIS
Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C
HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C
HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C
Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.05%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na ≤0.005%
Pb ≤0.001%
SO4 ≤0.05%
Zn ≤0.0005%
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CAT NO
HR2-781
NAME
DESCRIPTION
100 mL
PRICE
$155.00
cart quote
CAT NO
HR2-783
NAME
DESCRIPTION
100 mL
PRICE
$155.00
cart quote
Support Material(s)
HR2-781 BIS-TRIS pH 5.5 SDS
HR2-783 BIS-TRIS pH 6.5 SDSCertificate Of Analysis
Related Item(S)
- Hydrochloric acid
- StockOptions Bis-Tris Buffer Kit
- Individual StockOptions Bis-Tris Reagents
References
1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.
2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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ClearSeal Film™ and Applicator蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Crystallization Plates, Hardware & Accessories > Sealing Films, Tapes, Mats & Covers > ClearSeal Film™ and Applicator
ClearSeal Film™ and Applicator
Applications
- Sealing film used to seal sitting drop crystallization experiments
Features
- High optical clarity, low auto-fluorescence
- Handling and plate positioning is simplified as a result of the non-tacky adhesive
- Pressure sensitive adhesive
- For best results use specially designed applicator
- UV transmissable, compatible with UV imaging systems
- Low birefringence
- Certified DNase-, RNase-, and nucleic-acid-free
- Silicone adhesive is non-tacky until pressed against the sealing surface with the applicator
- Chemically inert; no extractable except at extreme pH
- Excellent chemical resistance (e.g. DMSO, Methanol, Acetonitrile)
- Heat and cold resistant, recommended for temperatures from -70°C and 110°C
- Complimentary sealing paddle is included in each box
Description
ClearSeal Film fits all SBS format 96 well plates.
Minimal evaporation, minimal cross-well contamination, easy to handle, and optically clear. Single coated, 2 mil (0.05 mm), optically clear polyolefin film with pressure-sensitive silicone-based adhesive. Supplied as a 140 mm x 78 mm film with white polyester release liner (backing). Actual seal area after end tabs removed is 116 x 78 mm Seals well to polypropylene, polystyrene, and polycarbonate plates. Compatible with aqueous and organic solvents. Suitable for use between -70 and 110°C. For best seal, the plate must be sealed with the Film Sealing Paddle HR4-513. ClearSeal Film has a limited shelf life. Must be used before expiration date printed on box.
Each pack of film (HR4-521) includes one free Film Sealing Paddle (available separately as HR4-413). HR4-413 Film Sealing Paddle, 3 7/8″ x 2 15/16″, polyethylene, white. 5 paddles per pack. An alternative to the plate roller or brayer for pressing films to assure a secure uniform seal around all wells on 96-well, 384-well, and 1536-well plates. These paddles are especially recommended for sealing films on raised-rim plates because they fit within the rim of the plate.
Store ClearSeal Film at 20°C +/- 10 degrees at 50% RH +/- 10%.
Helpful Tips
- Sufficient pressure must be applied to activate the silicone adhesive
- Multiple swipes of the sealing paddle in both directions is highly recommended
- A 10 minute or greater wait period before using for thermal cycling increases the film-to-plate bond
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CAT NO
HR4-521
NAME
DESCRIPTION
100 pack
PRICE
$190.00
cart quote
Related Item(S)
- Film Sealing Paddle
References
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Crystal Crusher蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Tools & Seeding > Crystal Crusher > Crystal Crusher
Crystal Crusher
Applications
- Crush macro crystals to create micro crystals
Features
- Solid, borosilicate glass tool and handle
- Smooth, hemispherical tip, will not damage crystallization plate
- Prepare micro crystals for seed stock for Microseed Matrix Screening(MMS)
Description
The Crystal Crusher is designed to crush macro crystals into micro crystals for seeding and other applications. The hemispherical end of the tool fits round, concave bottomed sitting drop crystallization plates for efficient crystal crushing. The Crystal Crusher is molded from solid, borosilicate glass. One end of the tool features a larger diameter, cylindrical handle, while the opposite end features a smooth, hemispherical end for crushing crystals without damaging crystallization plates.
Overall length: ~ 43 mm
Diameter of handle: ~ 3 mm
Diameter of hemispherical end: ~ 1 mm
Five Crystal Crushers per pack.
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CAT NO
HR4-216
NAME
DESCRIPTION
5 pack
PRICE
$23.00
cart quote
Support Material(s)
HR4-216 Crystal Crusher User GuideRelated Item(S)
- Seed Bead™ Kits
- Crystal Clear Sealing Tape
- X-Acto Gripster Knife
References
1. Microseed matrix screening for optimization in protein crystallization: what have we learned? D’Arcy A, Bergfors T, Cowan-Jacob SW, Marsh M. Acta Crystallogr F Struct Biol Commun. 2014 Sep;70 (Pt 9):1117-26. doi: 10.1107/S2053230X14015507. Epub 2014 Aug 29.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Methods & Results in Crystallization of Membrane Proteins蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Books > Books > Methods & Results in Crystallization of Membrane Proteins
Methods & Results in Crystallization of Membrane Proteins
Features
- Editor: So Iwata
- Publisher: International University Line, 2002
- ISBN-10: 0963681796
- ISBN-13: 978-0963681799
- Hardcover: 380 pages
Description
Sorry, Methods & Results in Crystallization of Membrane Proteins is no longer available. We’ve left this page up for reference only.
This text consists of four parts. Parts 1 and 2 include an introduction as well as general principles and techniques in membrane protein crystallization. This is followed by chapters covering the use of detergents, crystallization in lipidic cubic phases, the use and generation of antibody fragments, porin as a model, and crystallization of membrane proteins in oils. Part 3 focuses on specific examples of membrane proteins whose structures have been solved. Part 4 covers the design of a kit for membrane protein crystallization.
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Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Duco Cement蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Capillaries, Mounts & Supplies > Capillary Sealants > Duco Cement
Duco Cement
Applications
- Sealing capillaries
- Mount crystals to glass fibers
Description
Old school cement for sealing glass capillaries when mounting crystals. Easy to apply. Requires no heat. Fast-drying, medium-strength cement dries clear. Bonds glass, wood, some plastics, and metal. Not recommended for most polyethylene or polypropylene plastics. Sets in 5 minutes, item can be handled in 1 hour, full bond strength in 16 hours. 1 ounce (29.5 ml) tube.
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CAT NO
HR4-320
NAME
DESCRIPTION
29.5 ml tube
PRICE
$8.00
cart quote
Support Material(s)
HR4-320 Duco Cement SDSRelated Item(S)
- Glass Number 50 Capillaries
- Special Glass 10 Capillaries
- Quartz Capillaries
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Macromolecular Crystallization, Methods, Volume 34, Number 3蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Books > Books > Macromolecular Crystallization, Methods, Volume 34, Number 3
Macromolecular Crystallization, Methods, Volume 34, Number 3
Features
- Edited by: Alexander McPherson
- Publisher: Elsevier, Methods, Volume 34, Number 3, November 2004
- ISSN: 1046-2023
- Paperback: 172 pages
Description
This is a single issue of Methods, a companion journal to Methods in Enzymology, focusing on rapidly developing techniques. This single issue is dedicated to Macromolecular Crystallization and begins with an Introduction to Protein Crystallization by Alexander McPherson.
The issue features the following additional thirteen articles:
•Protein crystallization and phase diagrams, Neer Asherie.
•Growth and disorder of macromolecular crystals: insights from atomic force microscopy and x-ray diffraction studies, Alexander J. Malkin and Robert E. Thorne.
•Ions from the Hofmeister series and osmolytes: effects on proteins in solution and in the crystallization process, Kim D. Collins.
•Effects of naturally occurring osmolytes on protein stability and solubility; issues important in protein crystallization, D.W. Bolen.
•Practical aspects of using the microbatch method in screening conditions for protein crystallization, Allan D’Arcy, Aengus Mac Sweeney, and Alexander Haber.
•Automated systems for protein crystallization, Joel Bard, Kimberly Ercolani, Kristine Svenson, Andrea Olland, and Will Somers.
•Lipidic cubic phases as matrices for membrane protein crystallization, Peter Nollert.
•The use of recombinant and molecular engineering in protein crystallization, Zygmunt Derewenda.
•A pedestrian guide to membrane protein crystallization, Michael Wiener.
•Crystallization data mining in structural genomics: using positive and negative results to optimize protein crystallization screens, Rebecca Page and Raymond C. Stevens.
•Predictive models for protein crystallization, Bernhard Rupp and Junwen Wang.
•Crystallization of RNA and RNA-protein complexes, Ailong Ke and Jennifer A. Doudna.
•Macromolecular cryocrystallography – methods for cooling and mounting protein crystals are cryogenic temperatures, J.W. Pflugrath
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CAT NO
HR8-061
NAME
DESCRIPTION
each
PRICE
$46.00
cart quote
Related Item(S)
- Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Individual Grid Screen PEG 6000 Reagents蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > Grid Screen PEG 6000 > Individual Grid Screen PEG 6000 Reagents
Individual Grid Screen PEG 6000 Reagents
Applications
- Individual Grid Screen PEG 6000 reagents for screening, optimization and production
Features
- 185 ml volume
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-926-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-926-A1
Click to Zoom In
CAT NO
HR2-926-A1
NAME
DESCRIPTION
0.1 M Citric acid pH 4.0, 5% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-A2
NAME
DESCRIPTION
0.1 M Citric acid pH 5.0, 5% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-A3
NAME
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 5% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-A4
NAME
DESCRIPTION
0.1 M HEPES pH 7.0, 5% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-A5
NAME
DESCRIPTION
0.1 M Tris pH 8.0, 5% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-A6
NAME
DESCRIPTION
0.1 M BICINE pH 9.0, 5% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-B1
NAME
DESCRIPTION
0.1 M Citric acid pH 4.0, 10% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-B2
NAME
DESCRIPTION
0.1 M Citric acid pH 5.0, 10% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-B3
NAME
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 10% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-B4
NAME
DESCRIPTION
0.1 M HEPES pH 7.0, 10% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-B5
NAME
DESCRIPTION
0.1 M Tris pH 8.0, 10% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-B6
NAME
DESCRIPTION
0.1 M BICINE pH 9.0, 10% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-C1
NAME
DESCRIPTION
0.1 M Citric acid pH 4.0, 20% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-C2
NAME
DESCRIPTION
0.1 M Citric acid pH 5.0, 20% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-C3
NAME
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 20% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-C4
NAME
DESCRIPTION
0.1 M HEPES pH 7.0, 20% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-C5
NAME
DESCRIPTION
0.1 M Tris pH 8.0, 20% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-C6
NAME
DESCRIPTION
0.1 M BICINE pH 9.0, 20% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-D1
NAME
DESCRIPTION
0.1 M Citric acid pH 4.0, 30% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-D2
NAME
DESCRIPTION
0.1 M Citric acid pH 5.0, 30% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-D3
NAME
DESCRIPTION
0.1 M MES monohydrate pH 6.0, 30% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-D4
NAME
DESCRIPTION
0.1 M HEPES pH 7.0, 30% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-D5
NAME
DESCRIPTION
0.1 M Tris pH 8.0, 30% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
CAT NO
HR2-926-D6
NAME
DESCRIPTION
0.1 M BICINE pH 9.0, 30% w/v Polyethylene glycol 6,000
PRICE
$171.00
cart quote
Support Material(s)
HR2-926-** Individual Grid Screen PEG 6000 Reagents SDSRelated Item(S)
- Grid Screen PEG 6000
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Cobalt(II) chloride hexahydrate蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Cobalt(II) chloride hexahydrate
Cobalt(II) chloride hexahydrate
Applications
- Crystallization grade Cobalt(II) chloride hexahydrate for formulating screens or for optimization
Features
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Cobalt(II) chloride hexahydrate
Synonyms: Cobaltous chloride hexahydrate
Cl2Co • 6H2O
Mr 237.93
CAS Number [7791-13-1]
EC Number 231-589-4
Merck 14,2437
RTECS GG0200000
MDL Number MFCD00149652
UN Number UN 3260 8/PG 2
Purity ≥ 98.0%
Appearance (Starting Material): Violet, Crystals
Appearance (Solution): Very deep red
Measured pH Range: 2.1 – 4.6 at 25°C
Measured Conductivity Range: 103.4 – 114.8 mS/cm at 25°C
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CAT NO
HR2-713
NAME
DESCRIPTION
100 mL
PRICE
$79.00
cart quote
Support Material(s)
HR2-713 Cobalt(II) chloride hexahydrate SDSCertificate Of Analysis
References
1. Crystallization of tRNA ‘Leu’ from escherichia coli after purification with hydroxyapatite. Spencer, M; Neidle, S; Jones, TA. Biochem Biophys Res Commun 86, 66-70, 1979.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Harlan Teklad动物饲料Basal mixes
上海金畔生物科技有限公司代理Harlan Teklad动物饲料全线产品，欢迎访问Harlan Teklad动物饲料官网了解更多信息。
Basal mixes
You may want to add some specific ingredients in your own facility. In that case, Envigo provides basal mixes that are, by design, a starting point for additional inclusions. When you choose a basal mix diet, you simply add any ingredient(s) in a specific proportion that your require. The nutrient levels in our basal mix are adjusted to account for the dilution of the added ingredient(s), so that the final complete diet contains the desired nutrient levels.
Here are some examples of how a basal mix could be helpful to you:
- Leave all or a portion of the fat source out. The end user can add various fats and make multiple diets from one basal mix
- Make fresh diet as needed for an unstable, hazardous, or limited supply ingredient
- Add a different vitamin or mineral mix to the same basal mix to create a series of vitamin or mineral adjusted diets
If proper mixing equipment is available, and you have the capability to feed powder diet, basal mixes offer flexibility and economy.
Sodium acetate trihydrate Buffer蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Buffers > Sodium acetate trihydrate Buffer
Sodium acetate trihydrate Buffer
Applications
- Crystallization grade Sodium acetate trihydrate buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Formulated from Sodium acetate trihydrate
Synonyms: Acetic acid sodium salt trihydrate
C2H3NaO2 • 3H2O
CH3COONa • 3H2O
Mr 136.08
CAS Number [6131-90-4]
EC Number 204-823-8
Merck 14,8571
Beilstein Registry Number 3732037
RTECS AJ4580000
MDL Number MFCD00071557
PubChem Substance ID 24886015
Purity ≥ 99.5%
pKa = 4.8
The useful pH range of a Sodium acetate trihydrate buffer is pH 3.6 – 5.6.
Titrate HR2-569 to the desired pH using HCl
HR2-569 Measured pH Range: 8.7 – 9.3 at 25°C
HR2-569 Measured Conductivity Range: 48.0 – 51.7 mS/cm at 25°C
HR2-569 Measured Refractive Index Range: 1.34395 – 1.34415 at 20°C
HR2-789 has been titrated to pH 4.5 using HCl
HR2-789 Measured Conductivity Range: 60.8 – 67.7 mS/cm at 25°C
HR2-789 Measured Refractive Index Range: 1.34530 – 1.34579 at 20°C
HR2-731 has been titrated to pH 4.6 using HCl
HR2-731 Measured Conductivity Range: 59.3 – 64.5 mS/cm at 25°C
HR2-731 Measured Refractive Index Range: 1.34535 – 1.345745 at 20°C
Al ≤0.0005%
As ≤0.00001%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni ≤0.0005%
Pb ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.002%
Sr ≤0.0005%
Zn ≤0.0005%
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CAT NO
HR2-569
NAME
DESCRIPTION
100 mL
PRICE
$37.00
cart quote
CAT NO
HR2-789
NAME
DESCRIPTION
100 mL
PRICE
$37.00
cart quote
CAT NO
HR2-731
NAME
DESCRIPTION
100 mL
PRICE
$37.00
cart quote
Support Material(s)
HR2-569 Sodium acetate trihydrate solution SDS
HR2-789 Sodium acetate trihydrate pH 4.5 solution SDS
HR2-731 Sodium acetate trihydrate pH 4.6 solution SDS
Titration Table Certificate Of Analysis
Related Item(S)
- Hydrochloric acid
- StockOptions Sodium Acetate Buffer Kit
- Individual StockOptions Sodium Acetate Reagents
References
1. Crystal structure of thioltransferase at 2.2 A resolution. Katti, SK; Robbins, AH; Yang, Y; Wells, WW. Protein Sci 4, 1998- 2005, 1995.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Harlan Teklad动物饲料Vitamin adjusted diets
上海金畔生物科技有限公司代理Harlan Teklad动物饲料全线产品，欢迎访问Harlan Teklad动物饲料官网了解更多信息。
Vitamin adjusted diets
A few vitamin adjusted formulas are shown below. Please contact us for additional formulas of this nature or for more information about altering the vitamin profile of a diet, and please read below to learn more.
Most vitamin deficient diets use vitamin-free test casein (VFT casein) as the protein source. VFT casein is casein that has been extracted with denatured alcohol to reduce the fat content from about 1% to 0.1%. In the process the content of a number of vitamins (present at low or trace levels in casein) is further reduced, making this an ideal protein source for these types of diets. Envigo produces our own VFT in-house, and we also offer this as an ingredient for those mixing their own diets.
From this vitamin deficient base, various levels of vitamins can be added back. Many formulas adjust multiple vitamins, and other nutrients.
If use of an intact protein source is not advisable, an amino acid defined diet can be used.
Contact us for other formula examples or to discuss your specific needs.
Crystal Screen • Crystal Screen 2 • Crystal Screen HT蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Crystallization Screens > Crystal Screen • Crystal Screen 2 > Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Crystal Screen • Crystal Screen 2 • Crystal Screen HT
Applications
- Primary screen for proteins, soluble peptides, nucleic acids, & water soluble small molecules
- Sparse matrix additive screen
Features
- The original sparse matrix screen
- Sparse matrix formula efficiently samples salts, polymers, organics, & pH
- Proven effective with more than 1,000 biological macromolecules
- Tube or Deep Well block format
Description
The Crystal Screen and Crystal Screen 2 reagent kits are designed to provide a highly effective and rapid screening method for the crystallization of macromolecules. The screens are simple and practical for finding initial crystallization conditions. The initial crystallization conditions for more than 1,000 proteins, peptides, oligonucleotides, and small molecules have been determined using Crystal Screen.
A highly effective approach to overcome the exhaustive search for suitable crystallization conditions is the use of a sparse matrix method of trial conditions that is biased and selected from known crystallization conditions for macromolecules. The formulation utilized in Crystal Screen and Crystal Screen 2 evaluates 96 unique mixtures of pH, salts, polymers and organics, and their ability to promote crystal growth.
Crystal Screen contains 50 unique reagents, 10 ml each and is based on the sparse matrix formulation first described by Jancarik and Kim in 1991.
Crystal Screen 2, an extension of Crystal Screen, contains 48 unique reagents, 10 ml each and is based on the formulation first described by Cudney et al in 1994.
Crystal Screen HT contains 1 ml each of reagents 1-48 from Crystal Screen and all 48 reagents from Crystal Screen 2 in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
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CAT NO
HR2-110
NAME
DESCRIPTION
10 ml, tube format
PRICE
$253.00
cart quote
CAT NO
HR2-112
NAME
DESCRIPTION
10 ml, tube format
PRICE
$253.00
cart quote
CAT NO
HR2-130
NAME
DESCRIPTION
1 ml, Deep Well block format
PRICE
$147.00
cart quote
Support Material(s)
HR2-110 Crystal Screen Documents
HR2-110 Crystal Screen SDS
HR2-112 Crystal Screen 2 Documents
HR2-112 Crystal Screen 2 SDS
HR2-130 Crystal Screen HT Documents
HR2-130 Crystal Screen HT SDS
Crystal Screen Formulation & Scoring Data Related Item(S)
- Individual Crystal Screen • Crystal Screen 2 • Crystal Screen HT Reagents
References
1. Jancarik, J. & Kim, S.H. J. Appl. Cryst. 24, 409-411, (1991).
2. Cudney, R., Patel, S., Weisgraber, K., Newhouse, Y., and McPherson, A., Acta Cryst. (1994) D50, 414-423.
3. Expression, purification, crystallization and preliminary X-ray analysis of two arginine-biosynthetic enzymes from Mycobacterium tuberculosis. F. Moradian, C. Garen, L. Cherney, M. Cherney and M. N. G. James. Acta Cryst. (2006). F62, 986-988.
4. The development and application of a method to quantify the quality of cryoprotectant solutions using standard area-detector X-ray images. McFerrin and Snell, J. Appl. Cryst. (2002). 35, 538-545.
5. Get phases from arsenic anomalous scattering: de novo SAD phasing of two protein structures crystallized in cacodylate buffer. Liu X, Zhang H, Wang XJ, Li LF, Su XD. PLoS One. 2011;6(9):e24227. Epub 2011 Sep 2.
6. Cloning, crystallization and preliminary X-ray diffraction analysis of an intact DNA methyltransferase of a type I restriction-modification enzyme from Vibrio vulnificus. L. Huynh Thi Yen, S.-Y. Park and J.-S. Kim. Acta Cryst. (2014). F70, 489-492 [ doi:10.1107/S2053230X14004543 ]
7. Crystallization and preliminary X-ray analysis of a novel type of lipolytic hydrolase from Bacillus licheniformis. H. Ju, R. Pandian, K. Kim, K. K. Kim and T. D. Kim. Acta Cryst. (2014). F70, 473-475 [ doi:10.1107/S2053230X14004142 ]
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Adjustable Crystal Mount蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Capillaries, Mounts & Supplies > Magnetic Mounts & Supports > Adjustable Crystal Mount
Adjustable Crystal Mount
Applications
- Adjustable, magnetic crystal mounting with capillaries or fibers
Features
- Adjustable Z
- Magnetic mount
- Fits XYZ Goniometer Heads with Z Platform or magnetic base
- Fast, easy, and convenient crystal positioning and retrieval
Description
The Adjustable Crystal Mount is a magnetic piece (approximately 12 mm in height, 9.8 mm in diameter) designed to position a brass specimen pin and either a capillary or glass fiber onto a goniometer head for x-ray data collection.
The Adjustable Crystal Mount is engineered to fit onto the Z Platform or magnetic base within a goniometer head. The recessed bottom properly positions the crystal mount over the center of the goniometer head and prevents the mount from sliding across the goniometer head during data collection. The bottom of the crystal mount is flat and closed to prevent the brass specimen pin from protruding through the bottom of the mount. The magnetic design allows for quick, convenient, and rapid placement and retrieval of the crystal mount. Finally, the crystal mount is designed with a single allen screw which allows the Z-translation of the brass specimen pin to be adjusted (up and down) and then secured in position by tightening the screw using a goniometer head key or 0.88 mm allen key.
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CAT NO
HR8-028
NAME
DESCRIPTION
5 pack
PRICE
$71.00
cart quote
Related Item(S)
- Glass Number 50 Capillaries
- Special Glass 10 Capillaries
- Quartz Capillaries
- Brass Specimen Pin
- Magnetic Base
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Polyethylene glycol 20,000蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol 20,000
Polyethylene glycol 20,000
Applications
- Crystallization grade Polyethylene glycol 20,000 for formulating screens or for optimization
Features
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Polyethylene glycol 20,000
Synonyms: PEG 20,000
H(OCH2CH2)nOH
Mr ~ 16,000-24,000
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ4110000
MDL Number MFCD00081839
PubChem Substance ID 24887748
Melting point (Starting material) 63.0-66.0°C
Aldehyde None detected
Peroxide None detected
Measured Conductivity Range: 42.1 – 76.9 µS/cm at 25°C
Measured Refractive Index Range: 1.37413 – 1.37495 at 20°C
HR2-609 is sterile filtered and purged with dry argon.
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CAT NO
HR2-609
NAME
DESCRIPTION
200 mL
PRICE
$100.00
cart quote
Support Material(s)
HR2-609 Polyethylene glycol 20,000 SDSCertificate Of Analysis
References
1. Crystallization and preliminary crystallographic studies of an alpha-amylase inhibitor from wheat (Triticum aestivum). Oliva, G; Iulek, J; Ida, EI. Acta Crystallogr D 52, 1016- 1017, 1996.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Individual PEG/Ionic Liquid 1 • PEG/Ionic Liquid 2 • PEG/Ionic Liquid HT Reagents蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > PEG/Ionic Liquid 1 • PEG/Ionic Liquid 2 > Individual PEG/Ionic Liquid 1 • PEG/Ionic Liquid 2 • PEG/Ionic Liquid HT Reagents
Individual PEG/Ionic Liquid 1 • PEG/Ionic Liquid 2 • PEG/Ionic Liquid HT Reagents
Applications
- Individual reagents from PEG/Ionic Liquid 1, PEG/Ionic Liquid 2, and PEG/Ionic Liquid HT for screening, optimization, and production.
Features
- 185 ml
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-078-** and HR2-079-**
** Refers to the reagent number in the kit.
For example, PEG/Ionic Liquid 1 reagent number 1 = PEG/Ionic Liquid HT reagent A1 = HR2-078-01
For example, PEG/Ionic Liquid 2 reagent number 1 = PEG/Ionic Liquid HT reagent E1 = HR2-079-01
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CAT NO
HR2-078-01
NAME
DESCRIPTION
5% w/v Tetraethylammonium bromide, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-02
NAME
DESCRIPTION
5% w/v Tetraethylammonium bromide, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-03
NAME
DESCRIPTION
5% w/v Tetraethylammonium bromide, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-04
NAME
DESCRIPTION
5% w/v Tetraethylammonium bromide, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-05
NAME
DESCRIPTION
5% w/v Benzyltriethylammonium chloride, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-06
NAME
DESCRIPTION
5% w/v Benzyltriethylammonium chloride, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-07
NAME
DESCRIPTION
5% w/v Benzyltriethylammonium chloride, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-08
NAME
DESCRIPTION
5% w/v Benzyltriethylammonium chloride, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-09
NAME
DESCRIPTION
5% w/v 2-Hydroxyethylammonium formate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-10
NAME
DESCRIPTION
5% w/v 2-Hydroxyethylammonium formate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-11
NAME
DESCRIPTION
5% w/v 2-Hydroxyethylammonium formate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-12
NAME
DESCRIPTION
5% w/v 2-Hydroxyethylammonium formate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-13
NAME
DESCRIPTION
5% w/v Ethylammonium nitrate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-14
NAME
DESCRIPTION
5% w/v Ethylammonium nitrate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-15
NAME
DESCRIPTION
5% w/v Ethylammonium nitrate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-16
NAME
DESCRIPTION
5% w/v Ethylammonium nitrate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-17
NAME
DESCRIPTION
5% w/v Cholin acetate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-18
NAME
DESCRIPTION
5% w/v Cholin acetate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-19
NAME
DESCRIPTION
5% w/v Cholin acetate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-20
NAME
DESCRIPTION
5% w/v Cholin acetate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-21
NAME
DESCRIPTION
5% w/v Choline dihydrogen phosphate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-22
NAME
DESCRIPTION
5% w/v Choline dihydrogen phosphate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-23
NAME
DESCRIPTION
5% w/v Choline dihydrogen phosphate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-24
NAME
DESCRIPTION
5% w/v Choline dihydrogen phosphate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-25
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium acetate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-26
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium acetate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-27
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium acetate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-28
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium acetate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-29
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium chloride, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-30
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium chloride, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-31
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium chloride, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-32
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium chloride, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-33
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium chloride, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-34
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium chloride, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-35
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium chloride, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-36
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium chloride, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-37
NAME
DESCRIPTION
5% w/v 1-Hexyl-3-methylimidazolium chloride, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-38
NAME
DESCRIPTION
5% w/v 1-Hexyl-3-methylimidazolium chloride, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-39
NAME
DESCRIPTION
5% w/v 1-Hexyl-3-methylimidazolium chloride, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-40
NAME
DESCRIPTION
5% w/v 1-Hexyl-3-methylimidazolium chloride, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-41
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium dicyanamide, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-42
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium dicyanamide, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-43
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium dicyanamide, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-44
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium dicyanamide, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-45
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium dimethyl phosphate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-078-46
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium dimethyl phosphate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-47
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium dimethyl phosphate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-078-48
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium dimethyl phosphate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-01
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium methyl sulfate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-02
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium methyl sulfate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-03
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium methyl sulfate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-04
NAME
DESCRIPTION
5% w/v 1,3-Dimethylimidazolium methyl sulfate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-05
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium methyl sulfate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-06
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium methyl sulfate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-07
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium methyl sulfate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-08
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium methyl sulfate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-09
NAME
DESCRIPTION
5% w/v 1-n-Butyl-3-methylimidazolium n-octylsulfate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-10
NAME
DESCRIPTION
5% w/v 1-n-Butyl-3-methylimidazolium n-octylsulfate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-11
NAME
DESCRIPTION
5% w/v 1-n-Butyl-3-methylimidazolium n-octylsulfate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-12
NAME
DESCRIPTION
5% w/v 1-n-Butyl-3-methylimidazolium n-octylsulfate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-13
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium ethyl sulfate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-14
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium ethyl sulfate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-15
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium ethyl sulfate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-16
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium ethyl sulfate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-17
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium tetrafluoroborate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-18
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium tetrafluoroborate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-19
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium tetrafluoroborate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-20
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium tetrafluoroborate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-21
NAME
DESCRIPTION
5% w/v 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-22
NAME
DESCRIPTION
5% w/v 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-23
NAME
DESCRIPTION
5% w/v 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-24
NAME
DESCRIPTION
5% w/v 1-Butyl-2,3-dimethylimidazolium tetrafluoroborate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-25
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium tetrafluoroborate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-26
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium tetrafluoroborate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-27
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium tetrafluoroborate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-28
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium tetrafluoroborate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-29
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium trifluoroacetate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-30
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium trifluoroacetate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-31
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium trifluoroacetate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-32
NAME
DESCRIPTION
5% w/v 1-Butyl-3-methylimidazolium trifluoroacetate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-33
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium trifluoromethanesulfonate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-34
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium trifluoromethanesulfonate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-35
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium trifluoromethanesulfonate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-36
NAME
DESCRIPTION
5% w/v 1-Ethyl-3-methylimidazolium trifluoromethanesulfonate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-37
NAME
DESCRIPTION
5% w/v Tetrabutylphosphonium bromide, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-38
NAME
DESCRIPTION
5% w/v Tetrabutylphosphonium bromide, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-39
NAME
DESCRIPTION
5% w/v Tetrabutylphosphonium bromide, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-40
NAME
DESCRIPTION
5% w/v Tetrabutylphosphonium bromide, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-41
NAME
DESCRIPTION
5% w/v Triisobutylmethylphosphonium tosylate, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-42
NAME
DESCRIPTION
5% w/v Triisobutylmethylphosphonium tosylate, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-43
NAME
DESCRIPTION
5% w/v Triisobutylmethylphosphonium tosylate, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-44
NAME
DESCRIPTION
5% w/v Triisobutylmethylphosphonium tosylate, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-45
NAME
DESCRIPTION
5% w/v 1-Butylpyridinium chloride, 30% v/v Polyethylene glycol 400
PRICE
$155.00
cart quote
CAT NO
HR2-079-46
NAME
DESCRIPTION
5% w/v 1-Butylpyridinium chloride, 25% w/v Polyethylene glycol 1,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-47
NAME
DESCRIPTION
5% w/v 1-Butylpyridinium chloride, 20% w/v Polyethylene glycol 4,000
PRICE
$155.00
cart quote
CAT NO
HR2-079-48
NAME
DESCRIPTION
5% w/v 1-Butylpyridinium chloride, 15% w/v Polyethylene glycol 20,000
PRICE
$155.00
cart quote
HR2-078-** Individual PEG/Ionic Liquid 1 Reagents SDS
HR2-079-** Individual PEG/Ionic Liquid 2 Reagents SDS
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Individual StockOptions Tris Reagents蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > StockOptions Tris > Individual StockOptions Tris Reagents
Individual StockOptions Tris Reagents
Applications
- Individual StockOptions Tris reagents for screening, optimization and production
Features
- 1.0 M concentrated stocks
- 0.1 unit increments for fine tuning pH
- Available in pH 7.0 to 9.0
- 185 ml volume
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-900-**
** Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-900-01
1.0 M Tris (Tris titrated to indicated pH using HCl)
Synonym: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C
Useful pH range of Tris = 7.0 – 9.0
Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As = 5 ppm max
Ca = 5 ppm max
Cu = 5 ppm max
Fe = 5 ppm max
Pb = 5 ppm max
Mg = 5 ppm max
Use StockOptions Tris kit (HR2-100) for a set of 21 Tris reagents titrated to pH 7.0 to 9.0 in 0.1 pH increments.
Click to Zoom In
CAT NO
HR2-900-01
NAME
DESCRIPTION
1.0 M Tris pH 7.0
PRICE
$211.00
cart quote
CAT NO
HR2-900-02
NAME
DESCRIPTION
1.0 M Tris pH 7.1
PRICE
$211.00
cart quote
CAT NO
HR2-900-03
NAME
DESCRIPTION
1.0 M Tris pH 7.2
PRICE
$211.00
cart quote
CAT NO
HR2-900-04
NAME
DESCRIPTION
1.0 M Tris pH 7.3
PRICE
$211.00
cart quote
CAT NO
HR2-900-05
NAME
DESCRIPTION
1.0 M Tris pH 7.4
PRICE
$211.00
cart quote
CAT NO
HR2-900-06
NAME
DESCRIPTION
1.0 M Tris pH 7.5
PRICE
$211.00
cart quote
CAT NO
HR2-900-07
NAME
DESCRIPTION
1.0 M Tris pH 7.6
PRICE
$211.00
cart quote
CAT NO
HR2-900-08
NAME
DESCRIPTION
1.0 M Tris pH 7.7
PRICE
$211.00
cart quote
CAT NO
HR2-900-09
NAME
DESCRIPTION
1.0 M Tris pH 7.8
PRICE
$211.00
cart quote
CAT NO
HR2-900-10
NAME
DESCRIPTION
1.0 M Tris pH 7.9
PRICE
$211.00
cart quote
CAT NO
HR2-900-11
NAME
DESCRIPTION
1.0 M Tris pH 8.0
PRICE
$211.00
cart quote
CAT NO
HR2-900-12
NAME
DESCRIPTION
1.0 M Tris pH 8.1
PRICE
$211.00
cart quote
CAT NO
HR2-900-13
NAME
DESCRIPTION
1.0 M Tris pH 8.2
PRICE
$211.00
cart quote
CAT NO
HR2-900-14
NAME
DESCRIPTION
1.0 M Tris pH 8.3
PRICE
$211.00
cart quote
CAT NO
HR2-900-15
NAME
DESCRIPTION
1.0 M Tris pH 8.4
PRICE
$211.00
cart quote
CAT NO
HR2-900-16
NAME
DESCRIPTION
1.0 M Tris pH 8.5
PRICE
$211.00
cart quote
CAT NO
HR2-900-17
NAME
DESCRIPTION
1.0 M Tris pH 8.6
PRICE
$211.00
cart quote
CAT NO
HR2-900-18
NAME
DESCRIPTION
1.0 M Tris pH 8.7
PRICE
$211.00
cart quote
CAT NO
HR2-900-19
NAME
DESCRIPTION
1.0 M Tris pH 8.8
PRICE
$211.00
cart quote
CAT NO
HR2-900-20
NAME
DESCRIPTION
1.0 M Tris pH 8.9
PRICE
$211.00
cart quote
CAT NO
HR2-900-21
NAME
DESCRIPTION
1.0 M Tris pH 9.0
PRICE
$211.00
cart quote
Support Material(s)
HR2-900-** Individual StockOptions Tris Reagents SDSRelated Item(S)
- StockOptions Tris Buffer Kit
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Sodium tartrate dibasic dihydrate蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Sodium tartrate dibasic dihydrate
Sodium tartrate dibasic dihydrate
Applications
- Crystallization grade reagents for formulating screens or for optimization
Features
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Sodium tartrate dibasic dihydrate
Synonyms: L-(+)-Tartaric acid disodium salt or Disodium tartrate dihydrate or Sodium tartrate dihydrate
C4H4Na2O6 • 2H2O
Mr 230.08
CAS [6106-24-7]
EC Number 212-773-3
Merck 14,8684
Beilstein Registry Number 6121732
MDL Number MFCD00150035
PubChem Substance ID 24899620
Purity ≥ 99.0%
Measured pH range: 7.5 – 8.8 at 25°C
Measured Conductivity Range: 67.9 – 71.4 mS/cm at 25°C
Measured Refractive Index Range: 1.37544 – 1.37625 at 20°C
Total Impurities ≤0.005% insoluble matter passes filter test
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.0005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Ni ≤0.0005%
Pb ≤0.0005%
PO4 ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%
Click to Zoom In
CAT NO
HR2-677
NAME
DESCRIPTION
200 mL
PRICE
$107.00
cart quote
Support Material(s)
HR2-677 Sodium tartrate dibasic dihydrate SDSCertificate Of Analysis
References
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Polyethylene glycol monomethyl ether 5,000蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Polymers > Polyethylene glycol monomethyl ether 5,000
Polyethylene glycol monomethyl ether 5,000
Applications
- Crystallization grade Polyethylene glycol monomethyl ether 5,000 for formulating screens or for optimization
Features
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Polyethylene glycol 5,000 monomethyl ether
Synonyms: Methoxy poly(ethylene) glycol 5,000 or mono-Methyl polyethylene glycol 5,000 or PEG MME 5,000, or MPEG 5,000
CH3O(CH2CH2O)nH
CAS Number [9004-74-4]
Average Mn 5,000
EC Number 215-801-2
RTECS PE8271000
MDL Number MFCD00084416
PubChem Substance ID 24887753
Melting Point (Starting Material) 59°C
Appearance (Starting Material) Fine, white flakes
Aldehyde None detected
Peroxide None detected
Measured Conductivity range: 19.8 – 27.9 µS/cm at 25°C
Measured Refractive Index range: 1.40164 – 1.40215 at 20°C
Click to Zoom In
CAT NO
HR2-615
NAME
DESCRIPTION
200 mL
PRICE
$107.00
cart quote
Support Material(s)
HR2-615 Polyethylene glycol monomethyl ether 5,000 SDSCertificate Of Analysis
References
1. Poly(ethylene) glycol monomethyl ethers – an alternative to poly(ethylene) glycols in protein crystallization. A. M. Brzozowski and S. P. Tolley. Acta Cryst. (1994). D50, 466-468.
2. Crystallization of a Humicola lanuginosa lipase-inhibitor complex with the use of polyethylene glycol monomethyl ether. A. M. Brzozowski. Acta Cryst. (1993). D49, 352-354.
3. Crystallization and preliminary crystallographic analysis of the transcriptional regulator RfaH from Escherichia coli and its complex with ops DNA. M. N. Vassylyeva, V. Svetlov, S. Klyuyev, Y. D. Devedjiev, I. Artsimovitch and D. G. Vassylyev. Acta Cryst. (2006). F62, 1027-1030.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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PEGRx 1 • PEGRx 2 • PEGRx HT蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Crystallization Screens > PEGRx 1 • PEGRx 2 > PEGRx 1 • PEGRx 2 • PEGRx HT
PEGRx 1 • PEGRx 2 • PEGRx HT
Applications
- Primary and secondary, polymer and pH based crystallization screen for biological macromolecules
Features
- Developed at Hampton Research
- PEGRx 1 primary screen variables are polymer type (16 different polymers), polymer molecular weight, pH and low ionic strength.
- PEGRx 2 primary screen variables are polymer type (13 different polymers), polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.
- PEGRx HT combines PEGRx 1 and PEGRx 2 into a single 96 Deep Well block
- pH range 3.5 – 9, using 10 different buffer systems
- Polymer molecular weight range 200 to 20,000
- Ligand friendly, complex friendly
Description
PEGRx is a primary and secondary, polymer and pH based crystallization screen developed at Hampton Research.
It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.
PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.
PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.
PEGRx 1 contains 48 unique reagents, 10 ml each.
PEGRx 2 contains 48 unique reagents, 10 ml each.
PEGRx HT contains the 96 PEGRx 1 and PEGRx 2 reagent in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
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CAT NO
HR2-082
NAME
DESCRIPTION
10 ml, tube format
PRICE
$253.00
cart quote
CAT NO
HR2-084
NAME
DESCRIPTION
10 ml, tube format
PRICE
$253.00
cart quote
CAT NO
HR2-086
NAME
DESCRIPTION
1 ml, Deep Well block format
PRICE
$147.00
cart quote
Support Material(s)
HR2-082 PEGRx 1 Documents
HR2-082 PEGRx 1 SDS
HR2-084 PEGRx 2 Documents
HR2-084 PEGRx 2 SDS
HR2-086 PEGRx HT Documents
HR2-086 PEGRx HT SDS
Change in Formulation PEGRx Screens
PEGRx Formulation & Scoring Data Related Item(S)
- Individual PEGRx 1 • PEGRx 2 • PEGRx HT Reagents
References
1. Crystallization and preliminary X-ray crystallographic studies of DesR, a thermosensing response regulator in a two-component signalling system from Streptococcus pneumoniae. Ae Kyung Park, Seung Min Bong, Jin Ho Moon and Young Min Chi. Acta Cryst. (2009). F65.
2. Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors. Zhang L, Lin D, Sun X, Curth U, Drosten C, Sauerhering L, Becker S, Rox K, Hilgenfeld R. Science. 2020 Apr 24;368(6489):409-412. doi: 10.1126/science.abb3405. Epub 2020 Mar 20.
3. Structural insight into the electron transfer pathway of a self-sufficient P450 monooxygenase. Zhang, L., Xie, Z., Liu, Z. et al. Nat Commun 11, 2676 (2020). https://doi.org/10.1038/s41467-020-16500-5.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Microbatch 72 Well Plate (Greiner)蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Crystallization Plates, Hardware & Accessories > 72 Well Crystallization Plates > Microbatch 72 Well Plate (Greiner)
Microbatch 72 Well Plate (Greiner)
Applications
- Microbatch crystallization
Features
- Conical, flat bottom wells
- Hydrophobic or hydrophilic versions
- Compact size
- Supplied with cover
- Terasaki plate
Description
The 72 Well Microbatch Plate consists of a 6 by 12 well layout. Paraffin, Silicon, or Al’s Oil is poured into the trough area of the plate, submerging all 72 wells under oil. Sample and reagent are then pipetted into each of the wells. The plate is available as a plasma-treated, hydrophilic version for screening or an untreated, hydrophobic version for optimization. The hydrophilic version gives better liquid handling with small volumes, while the hydrophobic version reduces crystal nucleation and helps to prevent the crystals from sticking to the plastic.
• Plate dimensions: 83.3 mm x 58.0 mm x 10.0 mm
• Conical well with 11 µl drop volume
• Flat bottom well diameter: 1.3 mm
• Supplied with cover
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CAT NO
HR3-081
NAME
DESCRIPTION
untreated, hydrophobic – 270 plate case
PRICE
$254.00
cart quote
CAT NO
HR3-121
NAME
DESCRIPTION
treated, hydrophilic – 270 plate case
PRICE
$256.00
Temporarily Unavailable
More Available Soon!
Support Material(s)
Converting Vapor Diffusion to Microbatch Related Item(S)
- Paraffin & Silicon Oil
References
1. Crystallization and preliminary X-ray crystallographic analysis of a new crystal form of hydroxylamine oxidoreductase from Nitrosomonas europaea. Cedervall et al. Acta Cryst. (2009). F65, 1296-1298.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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X-Acto Gripster Knife蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Tools & Seeding > X-Acto Gripster Knife > X-Acto Gripster Knife
X-Acto Gripster Knife
Applications
- Cutting and removing sealing tape and film from crystallization plates
Features
- Sharp stainless steel blade
- Safety cap for blade
- Soft grip barrel
- Anti-roll design – will not roll off table or microscope
Description
Precisely cuts tapes and films around reservoir and drops from crystallization plates, safely and easily. Ergonomic soft rubberized barrel is firm and comfortable for better control. Easy to clean handle. Rear blade release is for easier and safer blade changing. Rear blade release makes blade changing safer, easier, and faster. Safety cap cover for blade. Anti-roll stop design – knife will not roll off table or microscope and stab you in the toe. Supplied with one #11 blade. Replacement blades available separately.
#11 Replacement Blade – Extremely sharp point for fine angle cutting. Stainless steel. Sold in a package of five blades.
X-Acto® is a registered trademark of Elmer’s Products, Inc.
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CAT NO
HR4-124
NAME
DESCRIPTION
Each
PRICE
$13.00
cart quote
CAT NO
HR4-126
NAME
DESCRIPTION
5/pk
PRICE
$9.00
cart quote
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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- Gallery
- My Account
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- Contact Us
- Quick Order
- Support
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- Privacy Policy
- Terms and Conditions
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- Products
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- My Account
- Support
- Contact Us
- Quick Order
- Privacy Policy
- Terms and Conditions
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© 2021 HAMPTON RESEARCH CORP.
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Individual Crystal Screen Cryo • Crystal Screen 2 Cryo • Crystal Screen Cryo HT Reagents蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Custom Shop Crystallization Reagents > Crystal Screen Cryo • Crystal Screen 2 Cryo > Individual Crystal Screen Cryo • Crystal Screen 2 Cryo • Crystal Screen Cryo HT Reagents
Individual Crystal Screen Cryo • Crystal Screen 2 Cryo • Crystal Screen Cryo HT Reagents
Applications
- Individual reagents from Crystal Screen Cryo • Crystal Screen 2 Cryo • Crystal Screen Cryo HT for screening, optimization and production
Features
- 185 ml volume
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
HR2-914-** or HR2-915-**
** = Refers to the reagent number in the kit.
For example, reagent number 1 = HR2-914-01
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CAT NO
HR2-914-01
NAME
DESCRIPTION
0.02 M Calcium chloride dihydrate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-914-02
NAME
DESCRIPTION
0.26 M Potassium sodium tartrate tetrahydrate, 35% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-03
NAME
DESCRIPTION
0.26 M Ammonium phosphate monobasic, 35% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-04
NAME
DESCRIPTION
0.075 M Tris hydrochloride pH 8.5, 1.5 M Ammonium sulfate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-05
NAME
DESCRIPTION
0.2 M Sodium citrate tribasic dihydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-914-06
NAME
DESCRIPTION
0.16 M Magnesium chloride hexahydrate, 0.08 M TRIS hydrochloride pH 8.5, 24% w/v Polyethylene glycol 4,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-07
NAME
DESCRIPTION
0.07 M Sodium cacodylate trihydrate pH 6.5, 0.98 M Sodium acetate trihydrate, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-08
NAME
DESCRIPTION
0.14 M Sodium citrate tribasic dihydrate, 0.07 M Sodium cacodylate trihydrate pH 6.5, 21% v/v 2-Propanol, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-09
NAME
DESCRIPTION
0.17 M Ammonium acetate, 0.085 M Sodium citrate tribasic dihydrate pH 5.6, 25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-10
NAME
DESCRIPTION
0.17 M Ammonium acetate, 0.085 M Sodium acetate trihydrate pH 4.6, 25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-11
NAME
DESCRIPTION
0.07 M Sodium citrate tribasic dihydrate pH 5.6, 0.7 M Ammonium phosphate monobasic, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-12
NAME
DESCRIPTION
0.18 M Magnesium chloride hexahydrate, 0.09 M HEPES sodium pH 7.5, 27% v/v 2-Propanol, 10% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-13
NAME
DESCRIPTION
0.2 M Sodium citrate tribasic dihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% v/v Polyethylene glycol 400
PRICE
$171.00
cart quote
CAT NO
HR2-914-14
NAME
DESCRIPTION
0.19 M Calcium chloride dihydrate, 0.095 M HEPES sodium pH 7.5, 26.6% v/v Polyethylene glycol 400, 5% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-15
NAME
DESCRIPTION
0.17 M Ammonium sulfate, 0.085 M Sodium cacodylate trihydrate pH 6.5, 25.5% w/v Polyethylene glycol 8,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-16
NAME
DESCRIPTION
0.075 M HEPES sodium pH 7.5, 1.125 M Lithium sulfate monohydrate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-17
NAME
DESCRIPTION
0.17 M Lithium sulfate monohydrate, 0.085 M TRIS hydrochloride pH 8.5, 25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-18
NAME
DESCRIPTION
0.16 M Magnesium acetate tetrahydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5, 16% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-19
NAME
DESCRIPTION
0.16 M Ammonium acetate, 0.08 M TRIS hydrochloride pH 8.5, 24% v/v 2-Propanol, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-20
NAME
DESCRIPTION
0.16 M Ammonium sulfate, 0.08 M Sodium acetate trihydrate pH 4.6, 20% w/v Polyethylene glycol 4,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-21
NAME
DESCRIPTION
0.2 M Magnesium acetate tetrahydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-914-22
NAME
DESCRIPTION
0.17 M Sodium acetate trihydrate, 0.085 M TRIS hydrochloride pH 8.5, 25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-23
NAME
DESCRIPTION
0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES sodium pH 7.5, 30% v/v Polyethylene glycol 400
PRICE
$171.00
cart quote
CAT NO
HR2-914-24
NAME
DESCRIPTION
0.14 M Calcium chloride dihydrate, 0.07 M Sodium acetate trihydrate pH 4.6, 14% v/v 2-Propanol, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-25
NAME
DESCRIPTION
0.07 M Imidazole pH 6.5, 0.7 M Sodium acetate trihydrate, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-26
NAME
DESCRIPTION
0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-914-27
NAME
DESCRIPTION
0.14 M Sodium citrate tribasic dihydrate, 0.07 M HEPES sodium pH 7.5, 14% v/v 2-Propanol, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-28
NAME
DESCRIPTION
0.17 M Sodium acetate trihydrate, 0.085 M Sodium cacodylate trihydrate pH 6.5, 25.5% w/v Polyethylene glycol 8,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-29
NAME
DESCRIPTION
0.065 M HEPES sodium pH 7.5, 0.52 M Potassium sodium tartrate tetrahydrate, 35% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-30
NAME
DESCRIPTION
0.17 M Ammonium sulfate, 25.5% w/v Polyethylene glycol 8,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-31
NAME
DESCRIPTION
0.17 M Ammonium sulfate, 25.5% w/v Polyethylene glycol 4,000, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-32
NAME
DESCRIPTION
1.5 M Ammonium sulfate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-33
NAME
DESCRIPTION
3.6 M Sodium formate, 10% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-34
NAME
DESCRIPTION
0.07 M Sodium acetate trihydrate pH 4.6, 1.4 M Sodium formate, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-35
NAME
DESCRIPTION
0.075 M HEPES sodium pH 7.5, 0.6 M Sodium phosphate monobasic monohydrate, 0.6 M Potassium phosphate monobasic, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-36
NAME
DESCRIPTION
0.065 M Tris hydrochloride pH 8.5, 5.2% w/v Polyethylene glycol 8,000, 35% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-37
NAME
DESCRIPTION
0.07 M Sodium acetate trihydrate pH 4.6, 5.6% w/v Polyethylene glycol 4,000, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-38
NAME
DESCRIPTION
0.09 M HEPES sodium pH 7.5, 1.26 M Sodium citrate tribasic dihydrate, 10% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-39
NAME
DESCRIPTION
0.085 M HEPES sodium pH 7.5, 1.7 M Ammonium sulfate, 1.7% w/v Polyethylene glycol 400, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-40
NAME
DESCRIPTION
0.095 M Sodium citrate tribasic dihydrate pH 5.6, 19% w/v Polyethylene glycol 4,000, 19% v/v 2-Propanol, 5% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-41
NAME
DESCRIPTION
0.085 M HEPES sodium pH 7.5, 17% w/v Polyethylene glycol 4,000, 8.5% v/v 2-Propanol, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-42
NAME
DESCRIPTION
0.04 M Potassium phosphate monobasic, 16% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-43
NAME
DESCRIPTION
24% w/v Polyethylene glycol 1,500, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-44
NAME
DESCRIPTION
0.1 M Magnesium formate dihydrate, 50% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-45
NAME
DESCRIPTION
0.16 M Zinc acetate dihydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5, 14.4% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-46
NAME
DESCRIPTION
0.16 M Calcium acetate hydrate, 0.08 M Sodium cacodylate trihydrate pH 6.5, 14.4% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-47
NAME
DESCRIPTION
0.08 M Sodium acetate trihydrate pH 4.6, 1.6 M Ammonium sulfate, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-48
NAME
DESCRIPTION
0.08 M Tris hydrochloride pH 8.5, 1.6 M Ammonium phosphate monobasic, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-49
NAME
DESCRIPTION
0.8 M Lithium sulfate monohydrate, 1.6% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-914-50
NAME
DESCRIPTION
0.4 M Lithium sulfate monohydrate, 12% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-01
NAME
DESCRIPTION
1.6 M Sodium chloride, 8% w/v Polyethylene glycol 6,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-02
NAME
DESCRIPTION
0.3 M Sodium chloride, 0.006 M Magnesium chloride hexahydrate, 0.006 M Hexadecyltrimethylammonium bromide, 40% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-03
NAME
DESCRIPTION
21.25% v/v Ethylene glycol, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-04
NAME
DESCRIPTION
26.25% v/v 1,4-Dioxane, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-05
NAME
DESCRIPTION
1.5 M Ammonium sulfate, 3.75% v/v 2-Propanol, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-06
NAME
DESCRIPTION
0.65 M Imidazole pH 7.0, 35% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-07
NAME
DESCRIPTION
8% w/v Polyethylene glycol 1,000, 8% w/v Polyethylene glycol 8,000, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-08
NAME
DESCRIPTION
1.05 M Sodium chloride, 7% v/v Ethanol, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-09
NAME
DESCRIPTION
0.075 M Sodium acetate trihydrate pH 4.6, 1.5 M Sodium chloride, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-10
NAME
DESCRIPTION
0.2 M Sodium chloride, 0.1 M Sodium acetate trihydrate pH 4.6, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-915-11
NAME
DESCRIPTION
0.008 M Cobalt(II) chloride hexahydrate, 0.08 M Sodium acetate trihydrate pH 4.6, 0.8 M 1,6-Hexanediol, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-12
NAME
DESCRIPTION
0.095 M Cadmium chloride hydrate, 0.095 M Sodium acetate trihydrate pH 4.6, 28.5% v/v Polyethylene glycol 400, 5% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-13
NAME
DESCRIPTION
0.18 M Ammonium sulfate, 0.09 M Sodium acetate trihydrate pH 4.6, 27% w/v Polyethylene glycol monomethyl ether 2,000, 10% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-14
NAME
DESCRIPTION
0.15 M Potassium sodium tartrate tetrahydrate, 0.075 M Sodium citrate tribasic dihydrate pH 5.6, 1.5 M Ammonium sulfate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-15
NAME
DESCRIPTION
0.375 M Ammonium sulfate, 0.075 M Sodium citrate tribasic dihydrate pH 5.6, 0.75 M Lithium sulfate monohydrate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-16
NAME
DESCRIPTION
0.375 M Ammonium sulfate, 0.075 M Sodium citrate tribasic dihydrate pH 5.6, 0.75 M Lithium sulfate monohydrate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-17
NAME
DESCRIPTION
0.08 M Sodium citrate tribasic dihydrate pH 5.6, 28% v/v tert-Butanol, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-18
NAME
DESCRIPTION
0.007 M Iron(III) chloride hexahydrate, 0.07 M Sodium citrate tribasic dihydrate pH 5.6, 7% v/v Jeffamine® M-600®, 30% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-19
NAME
DESCRIPTION
0.095 M Sodium citrate tribasic dihydrate pH 5.6, 2.375 M 1,6-Hexanediol, 5% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-20
NAME
DESCRIPTION
0.08 M MES monohydrate pH 6.5, 1.28 M Magnesium sulfate heptahydrate, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-21
NAME
DESCRIPTION
0.075 M Sodium phosphate monobasic monohydrate, 0.075 M Potassium phosphate monobasic, 0.075 M MES monohydrate pH 6.5, 1.5 M Sodium chloride, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-22
NAME
DESCRIPTION
0.065 M MES monohydrate pH 6.5, 7.8% w/v Polyethylene glycol 20,000, 35% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-23
NAME
DESCRIPTION
1.2 M Ammonium sulfate, 0.075 M MES monohydrate pH 6.5, 7.5% v/v 1,4-Dioxane, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-24
NAME
DESCRIPTION
0.05 M Cesium chloride, 0.1 M MES monohydrate pH 6.5, 30% v/v Jeffamine® M-600®
PRICE
$171.00
cart quote
CAT NO
HR2-915-25
NAME
DESCRIPTION
0.0075 M Cobalt(II) chloride hexahydrate, 0.075 M MES monohydrate pH 6.5, 1.35 M Ammonium sulfate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-26
NAME
DESCRIPTION
0.18 M Ammonium sulfate, 0.09 M MES monohydrate pH 6.5, 27% w/v Polyethylene glycol monomethyl ether 5,000, 10% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-27
NAME
DESCRIPTION
0.009 M Zinc sulfate heptahydrate, 0.09 M MES monohydrate pH 6.5, 22.5% v/v Polyethylene glycol monomethyl ether 550, 10% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-28
NAME
DESCRIPTION
1.6 M Sodium citrate tribasic dihydrate pH 6.5
PRICE
$171.00
cart quote
CAT NO
HR2-915-29
NAME
DESCRIPTION
0.5 M Ammonium sulfate, 0.1 M HEPES pH 7.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-915-30
NAME
DESCRIPTION
0.08 M HEPES pH 7.5, 8% w/v Polyethylene glycol 6,000, 4% v/v (+/-)-2-Methyl-2,4-pentanediol, 20% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-31
NAME
DESCRIPTION
0.085 M HEPES pH 7.5, 17% v/v Jeffamine® M-600®, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-32
NAME
DESCRIPTION
0.075 M Sodium chloride, 0.075 M HEPES pH 7.5, 1.2 M Ammonium sulfate, 25% v/v Glycerol
PRICE
$171.00
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CAT NO
HR2-915-33
NAME
DESCRIPTION
0.07 M HEPES pH 7.5, 1.4 M Ammonium formate, 30% v/v Glycerol
PRICE
$171.00
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CAT NO
HR2-915-34
NAME
DESCRIPTION
0.0375 M Cadmium sulfate hydrate, 0.075 M HEPES pH 7.5, 0.75 M Sodium acetate trihydrate, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-35
NAME
DESCRIPTION
0.1 M HEPES pH 7.5, 70% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
cart quote
CAT NO
HR2-915-36
NAME
DESCRIPTION
0.085 M HEPES pH 7.5, 3.655 M Sodium chloride, 15% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-37
NAME
DESCRIPTION
0.075 M HEPES pH 7.5, 7.5% w/v Polyethylene glycol 8,000, 6% v/v Ethylene glycol, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-38
NAME
DESCRIPTION
0.075 M HEPES pH 7.5, 15% w/v Polyethylene glycol 10,000, 25% v/v Glycerol
PRICE
$171.00
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CAT NO
HR2-915-39
NAME
DESCRIPTION
0.2 M Magnesium chloride hexahydrate, 0.1 M Tris pH 8.5, 3.4 M 1,6-Hexanediol
PRICE
$171.00
cart quote
CAT NO
HR2-915-40
NAME
DESCRIPTION
0.075 M Tris pH 8.5, 18.75% v/v tert-Butanol, 25% v/v Glycerol
PRICE
$171.00
cart quote
CAT NO
HR2-915-41
NAME
DESCRIPTION
0.0075 M Nickel(II) chloride hexahydrate, 0.075 M Tris pH 8.5, 0.75 M Lithium sulfate monohydrate, 25% v/v Glycerol
PRICE
$171.00
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CAT NO
HR2-915-42
NAME
DESCRIPTION
1.275 M Ammonium sulfate, 0.085 M Tris pH 8.5, 25.2% v/v Glycerol
PRICE
$171.00
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CAT NO
HR2-915-43
NAME
DESCRIPTION
0.2 M Ammonium phosphate monobasic, 0.1 M Tris pH 8.5, 50% v/v (+/-)-2-Methyl-2,4-pentanediol
PRICE
$171.00
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CAT NO
HR2-915-44
NAME
DESCRIPTION
0.075 M Tris pH 8.5, 15% v/v Ethanol, 25% v/v Glycerol
PRICE
$171.00
Potassium phosphate dibasic蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Salts > Potassium phosphate dibasic
Potassium phosphate dibasic
Applications
- Crystallization grade Potassium phosphate dibasic for formulating screens or for optimization
Features
- Sterile filtered solution
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Potassium phosphate dibasic
Synonyms: Dipotassium hydrogenphosphate or Dipotassium phosphate or sec.-Potassium phosphate or di-Potassium hydrogen phosphate anhydrous
K2HPO4
HK2O4P
Mr 174.18
CAS Number [7758-11-4]
EC Number 231-834-5
Merck 14,7658
MDL Number MFCD00011383
PubChem Substance ID 24881849
Purity ≥ 99.0%
pKa1 = 2.15 at 25°C
pka2 = 6.82 at 25°C
pka3 = 12.38 at 25°C
Measured pH Range: 9.5 – 9.8 at 25°C
Measured Conductivity Range: 126.9 – 154.8 mS/cm at 25°C
Measured Refractive Index Range: 1.40735 – 1.40877 at 20°C
Total Impurities: Insoluble matter passes filter test
Al ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Cl ≤0.005%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
Li ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.5%
Ni ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Sr ≤0.0005%
Zn ≤0.0005%
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CAT NO
HR2-635
NAME
DESCRIPTION
200 mL
PRICE
$93.00
cart quote
Support Material(s)
HR2-635 Potassium phosphate dibasic SDSCertificate Of Analysis
Related Item(S)
- Sodium potassium phosphate (Quik Optimize)
- Quik Screen
References
1. Preliminary crystallographic study of the Fab fragments of two monoclonal anti-2-phenyloxazolone antibodies. Mariuzza, RA; Boulot, G; Guillon, V; Poljak, RJ; Berek, C; Jarvis, JM; Milstein, C. J Biol Chem 260, 10268-10270, 1985.
2. New crystal form of the photosynthetic reaction centre from Rhodobacter sphaeroides of Improved diffraction quality. Buchanan, SK; Fritzsch, G; Ermler, U; Michel, H. J Mol Biol 230, 1311- 1314, 1993.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Seed Bead™ Kits蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Tools & Seeding > Seed Bead > Seed Bead™ Kits
Seed Bead™ Kits
Applications
- Generate seeds of protein crystals
Features
- Easily generate consistent seed stocks
- Use serial dilution to control the number of seeds introduced into the drop
- Microseed matrix screening (MMS) seed stock
- PTFE, Stainless Steel, Zirconium Silicate Ceramic, or Glass Seed Bead
Description
The Seed Bead kits are used to create a seed stock for performing subsequent seeding experiments. The Seed Bead kits contains 24 Seed Bead Tubes, a special 1.5 ml microcentrifuge tube, shaped to maintain the seed in the sample during mixing. Each tube is configured with a PTFE, Stainless Steel, Zirconium Silicate Ceramic, or Glass Seed Beads.
Seeding allows one to grow crystals in the metastable zone. Crystallization in this zone provides control, reproducibility and an improved likelihood of a successful crystallization experiment. Also, crystals can grow from seeds but cannot spontaneously nucleate. By placing a seed or solution of seeds in a drop which is saturated to the metastable zone, one can use the seeds to grow larger single crystals. By controlling the number of seeds introduced into the drop, one can control the number of crystals grown. It is not practically possible to measure and know the number of seeds introduced to a drop, but by performing serial dilutions from a concentrated seed stock, one can control the number of crystals grown in the drop.
Using the Seed Bead kits, one can create crystal seed stock for subsequent seeding experiments. Crystals are placed in the microcentrifuge tube with the Seed Bead(s) and either vortexed or sonicated to generate a seed stock. Then by performing serial dilutions, one can control the number and size of crystals in the experiment.
The Seed Bead kit is useful for the preparation of seed stocks for automated and semi-automated microseeding (D’Arcy 2007, Harlos 2008).
Each Seed Bead Kit (HR2-320) contains 24 special microcentrifuge tubes, each tube containing a single 3mm PTFE Seed Bead.
Each Seed Bead Steel Kit (HR4-780) contains 24 special microcentrifuge tubes, each tube containing a six 1.6 mm Stainless Steel Seed Beads.
Each Seed Bead Ceramic Kit (HR4-781) contains 24 special microcentrifuge tubes, each tube containing a ten 1.0 mm Zirconium Silicate Ceramic Seed Beads.
Each Seed Bead Glass Kit (HR4-782) contains 24 special microcentrifuge tubes, each tube containing a ten 1.0 mm Glass Seed Beads.
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CAT NO
HR2-320
NAME
DESCRIPTION
24 tubes with PTFE Seed Bead
PRICE
$71.00
cart quote
CAT NO
HR4-780
NAME
DESCRIPTION
24 tubes with Steel Seed Beads
PRICE
$71.00
cart quote
CAT NO
HR4-781
NAME
DESCRIPTION
24 tubes with Ceramic Seed Beads
PRICE
$71.00
cart quote
CAT NO
HR4-782
NAME
DESCRIPTION
24 tubes with Glass Seed Beads
PRICE
$71.00
cart quote
Support Material(s)
HR2-320 Seed Bead User Guide
HR4-780 Seed Bead Steel User Guide
HR4-781 Seed Bead Ceramic User Guide
HR4-782 Seed Bead Glass User Guide Related Item(S)
- Crystal Crusher
References
1. Stura, E.A., Wilson, I.A., Methods: A Companion to Methods in Enzymology (1990) 1, 38-49.
2. Stura, E.A., Wilson, I.A., “Seeding Techniques” in Crystallization of Nucleic Acids and Proteins: A Practical Approach. Oxford University Press (1992) 99-126.
3. A method to produce microseed stock for use in the crystallization of biological macromolecules Luft, J.R., DeTitta, G.T. Acta Cryst. (1999). D55, 988-993.
4. J.R. Luft and G.T. DeTitta, Methods in Enzymology (1997) 276, 110-131.
5. Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement. Watanabe et al. Acta Cryst. (2006). D62, 784-792.
6. Crystallization and preliminary crystallographic analysis of p40phox, a regulatory subunit of NADPH oxidase. K. Honbou, S. Yuzawa, N. N. Suzuki, Y. Fujioka, H. Sumimoto and F. Inagaki. Acta Cryst. (2006). F62, 1021-1023 (Used seed bead to optimize)
7. Purification, crystallization and preliminary X-ray diffraction study of human ribosomal protein L10 core domain. Yuji Kobayashi et al. Acta Cryst. (2007). F63, 950-952
8. An automated microseed matrix-screening method. Allan D′Arcy, Frederic Villard and May Marsh. Acta Cryst. (2007). D63, 550-554
9. The role of bias in crystallization conditions in automated microseeding. Edwin Pozharski et al. Acta Cryst. (2008). D64, 1222-1227.
10. Transmission electron microscopy for the evaluation and optimization of crystal growth. Hilary P. Stevenson & Guillermo Calero et al. Acta Cryst. (2016) D72, 603-615.
11. Microseed matrix screening for optimization in protein crystallization: what have we learned? D’Arcy A, Bergfors T, Cowan-Jacob SW, Marsh M. Acta Crystallogr F Struct Biol Commun. 2014 Sep;70 (Pt 9):1117-26. doi: 10.1107/S2053230X14015507. Epub 2014 Aug 29.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Solubility & Stability Screen蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimization Screens > Solubility & Stability Screen > Solubility & Stability Screen
Solubility & Stability Screen
Applications
- A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization
- Preformulation screening
- Sample buffer optimization for crystallization, cryo-electron microscopy, and NMR
Features
- Compatible with Thermofluor, Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays
- 96 sterile filtered reagents
- 17 classes of reagents and excipients
- Highly concentrated (10x) reagent formulation
- Developed at Hampton Research
Description
Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.
The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.
Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11 The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.
Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12
Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.
It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15 Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11
The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.
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CAT NO
HR2-072
NAME
DESCRIPTION
0.5 ml, Deep Well block format
PRICE
$276.00
cart quote
Support Material(s)
HR2-072 Solubility & Stability Screen Documents
HR2-072 Solubility & Stability Screen SDS
Solubility & Stability Screen Formulation and Scoring Data Related Item(S)
- Individual Solubility & Stability Reagents
References
1. Preparation and analysis of protein crystals. Alexander McPherson. 1982 John Wiley and Sons, Inc.
2. A straight-forward method of optimizing protein solubility for NMR. Peter W.A. Howe, Journal of Biomolecular NMR 30: 283-286, 2004.
3. Rapid determination of protein solubility and stability conditions for NMR studies using incomplete factorial design. Demene, H. et al. Journal of Biomolecular NMR (2006) 34: 137-151.
4. Dissolution of protein aggregation by small amine compounds. Takagai et al. Science and Technology of Advanced Materials 4 (2003) 55-59.
5. Detection and prevention of protein aggregation before, during, and after purification. Sarah E. Bondos and Alicia Bicknell. Analytical Biochemistry 316 (2003) 223-231.
6. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics. Sun et al. Anal Bioanal Chem (2010) DOI 10.1007/s00216-010-4381-5.
7. Stabilization of proteins by low molecular weight multi-ions. Middaugh et al. Journal of Pharmaceutical Sciences, Vol. 91, No. 10, October 2002.
8. Inhibition of aggregation side reactions during in vitro protein folding. Eliana De Bernardez Clark, Elisabeth Schwarz, Rainer Rudolph. Methods in Enzymology Volume 309 page 228 (1991).
9. Refolding a glutamine synthetase truncation mutant in vitro: Identifying superior conditions using a combination of chaperonins and osmolytes. Paul A. Voziyan, Lalita Jadhav, Mark T. Fisher. Journal of Pharmaceutical Science Volume 89 page 1036 (2000).
10. Chemical Chaperones Regulate Molecular Chaperones in Vitro and in Cells under Combined Salt and Heat Stresses. Goloubinoff et al. J. Biol. Chem., Vol. 276, Issue 43, 39586-39591, October 26, 2001.
11. Effect of additives on protein aggregation. Shiraki et al. Current Pharmaceutical Biotechnology, 2009, 10, 400-407.
12. A thermal stability assay can help to estimate the crystallization likelihood of biological samples. Marquez et al. Acta Cryst. (2011). D67, 915-919.
13. The combined use of the Thermofluor assay and ThermoQ analytical software for the determination of protein stability and buffer optimization as an aid in protein crystallization. Kevin Phillips and Andres Hernandez de la Pena. Current Protocols in Molecular Biology 10.28.1-10.28.15 April 2011.
14. Thermofluor-based optimization strategy for the stabilization and crystallization of Campylobacter jejuni desulforubrerythrin. Romao et al. Protein Expression and Purification 81 (2012) 193-200.
15. Optimization of protein solubility and stability for protein nuclear magnetic resonance. Bagby S, Tong KI, Ikura, M. Methods Enzymol. 2001;339:20-41.
16. High-Density Miniaturized Thermal Shift Assays as a General Strategy for Drug Discovery. Pantoliano et al, Journal of Biomolecular Screening, Volume 6, Number 6, 2001.
17. Thermofluor-based high-throughput stability optimization of proteins for structural studies. Ericsson, U.B. et al, Anal Biochem. 2006 Oct 15;357(2):289-98. Epub 2006 Aug 10.
18. Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination. Vedadi, M. et al, Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15835-40. Epub 2006 Oct 11.
19. Comparison of fluorescence and light scattering based methods to assess formation and stability of protein-protein complexes. J. Kopec, G. Schneider. Journal of Structural Biology 175 (2011) 216-223.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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Greiner ComboPlate and CrystalBridge蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Crystallization Plates, Hardware & Accessories > 24 Well Crystallization Plates > Greiner ComboPlate and CrystalBridge
Greiner ComboPlate and CrystalBridge
Applications
- Hanging and sitting drop crystallization
Features
- 24 reagent wells
- ComboPlate has flat, raised rings about each well to minimize spills and cross contamination
- ComboPlate seals with 18 mm glass or plastic cover slides
- Slightly raised protection lid
- Sitting drop when used with CrystalBridge
Description
The Greiner ComboPlate is a 24 well plate with a raised cover in an SBS microplate footprint. The plate, supplied without sealant, can be sealed with an 18 mm diameter cover slide and sealant for hanging drop crystallization. Supplied with cover.
Dimensions: 127.76 mm x 85.48 mm x 19 mm
Well depth: 16.5 mm
Well capacity: 3.3 ml
Typical fill volume: 0.5 – 1.0 ml
When used with the Greiner CrystalBridge, the ComboPlate can be used for sitting drop crystallization and sealed with sealing film/tape or 18 mm diameter cover slide and sealant. The Greiner CrystalBridge is a polystyrene sitting drop pedestal with a concave drop well (45 µl capacity) and is designed specifically for the ComboPlate.
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CAT NO
HR3-200
NAME
DESCRIPTION
24 plate case
PRICE
$59.00
Temporarily Unavailable
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CAT NO
HR3-150
NAME
DESCRIPTION
100 pack
PRICE
$82.00
cart quote
CAT NO
HR3-154
NAME
DESCRIPTION
400 pack
PRICE
$326.00
cart quote
Support Material(s)
Greiner ComboPlate Drawing
Greiner ComboPlate Datasheet
Greiner CrystalBridge DrawingRelated Item(S)
- Dow Corning® 7 Release Compound Grease
- Dow Corning® Vacuum Grease
- OptiClear Plastic Cover Slides
- 18 mm Siliconized Glass Cover Slides
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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MES monohydrate Buffer蛋白结晶试剂盒Hampton Research
Hampton Research蛋白结晶试剂盒
Products > Optimize Reagents > Optimize – Buffers > MES monohydrate Buffer
MES monohydrate Buffer
Applications
- Crystallization grade MES monohydrate for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
MES monohydrate
Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate
C6H13NO4S • H2O
Mr 213.25
CAS Number [145224-94-8]
EC Number 224-632-3
Beilstein Registry Number 329824236
RTECS QE3479500
MDL Number MFCD00149409
PubChem Substance ID 24885996
Purity ≥ 99.0%
pKa 6.1 at 25°C
Solutions are stable at 2-8°C for months. Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably). The identity of the yellow breakdown product is unknown. When MES solutions are stored at room temperature, they can turn yellow within six months.
Useful pH range of MES 5.2 – 7.1
Titrate HR2-587 to the desired pH using NaOH
HR2-587 Measured pH Range: 3.2 – 3.4 at 25°C
HR2-587 Measured Conductivity Range: 166.4 – 2440 µS/cm at 25°C
HR2-587 Measured Refractive Index Range: 1.34655 – 1.34731 at 20°C
HR2-787 has been titrated to pH 6.5 using NaOH
HR2-787 Measured Conductivity Range: 19.2 – 25.6 mS/cm at 25°C
HR2-787 Measured Refractive Index Range: 1.36113 – 1.36251 at 20°C
HR2-943-07 – MES monohydrate pH 5.8 – 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit.
Impurities:
Endotoxin ≤ 50 EU/g
Yeast and Mold ≤ 100 CFU/g
Bioburden ≤ 100 CFU/g
Al ≤ 5.0 ppm
As ≤ 3.0 ppm
Ba ≤ 5.0 ppm
Bi ≤ 5.0 ppm
Ca ≤ 20.0 ppm
Cd ≤ 5.0 ppm
Co ≤ 5.0 ppm
Cr ≤ 5.0 ppm
Cu ≤ 5.0 ppm
Fe ≤ 5.0 ppm
Li ≤ 5.0 ppm
Mg ≤ 5.0 ppm
Mn ≤ 5.0 ppm
Mo ≤ 5.0 ppm
Na ≤ 20.0 ppm
Ni ≤ 5.0 ppm
Pb ≤ 5.0 ppm
Sb ≤ 5.0 ppm
Zn ≤ 5.0 ppm
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CAT NO
HR2-587
NAME
DESCRIPTION
100 mL
PRICE
$129.00
cart quote
CAT NO
HR2-787
NAME
DESCRIPTION
100 mL
PRICE
$129.00
cart quote
Support Material(s)
HR2-587 MES monohydrate 0.5 M solution SDS
HR2-787 MES monohydrate pH 6.5 1.0 M solution SDS
Titration Table Certificate Of Analysis
Related Item(S)
- Sodium hydroxide
- StockOptions MES Buffer Kit
- Individual StockOptions MES Reagents
References
1. Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. Biochemistry 33, 1555- 1564, 1994.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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