TAQuest qPCR Master Mix with Helixyte Green *高ROX*

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

货号	17274	存储条件	在零下15度以下保存, 避免光照
规格	1 mL	价格	1164
Ex (nm)		Em (nm)
分子量		溶剂	Water
产品详细介绍

简要概述

产品基本信息

货号：17274

产品名称：TAQuest qPCR Master Mix with Helixyte Green *高ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

溶剂：水

 

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含大量ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *高ROX*。 

 

适用仪器

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qPCR
仪器规格	SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *高ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分	体积 (25 µL/reaction)	体积 (50 µL/reaction)	最终浓度
TAQuest qPCR Master Mix with Helixyte Green *高 ROX*	12.5 µL	25 µL	1X
上游引物，10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
下游引物，10 µM	0.25-2.5 µL	0.5-5.0 µL	0.1-1.0 µM
DNA模板	1-5 µL	1-5 µL	优化的浓度
无核酸酶水	25 µL	50     µL

表 2. 热循环参数

范围	聚合酶激活	PCR (30-40个循环)
	Hold	变性	退火	延伸
温度	95 °C	95 °C	55-65 °C	68-72 °C
时间 (m:ss)	0:20	0:30	1:00	1:00

 

参考文献

Aligned Expression of IFI16 and STING Genes in RRMS Patients’ Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Authors: Siddiqi, Allauddin and Milne, Trudy and Cullinan, Mary P and Seymour, Gregory J
Journal: Clinical oral implants research (2016): 288-94

Real-time immuno-PCR for ultrasensitive detection of pyrene and other homologous PAHs.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Zhang, Y Y and Qiao, B and Sun, Y and Yang, L and Hu, P and Lu, S Y and Ren, H L and Zhang, J H and Wang, X R and Liu, Z S
Journal: Biosensors & bioelectronics (2015): 42-7

Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Authors: Ramos, Carlos A N and Araújo, Flábio R and Souza, Ingrid I F and Bacanelli, G and Luiz, Hera L and Russi, Lívia S and Oliveira, Renato H M and Soares, Cleber O and Rosinha, Grácia M S and Alves, Leucio C
Journal: Veterinary parasitology (2011): 79-83