辣根过氧化物酶*针对缀合进行了优化*

	货号	11021	存储条件	在零下15度以下保存, 避免光照
	规格	10 mg	价格	960
	Ex (nm)		Em (nm)
	分子量		溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：11021

产品名称：辣根过氧化物酶

规格：10 mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

溶剂：水

产品介绍

辣根过氧化物酶（HRP）是一种糖蛋白，分子量约为44 KD。它从辣根（Amoracia Rustana）中分离出来，属于过氧化物酶的铁原卟啉组。 HRP是包含四个二硫键的单链多肽。它是一种糖蛋白，含有18％的碳水化合物。碳水化合物组合物由半乳糖，阿拉伯糖，木糖，岩藻糖，甘露糖，甘露糖胺和半乳糖胺组成。 HRP容易与过氧化氢（H2O2）结合，生成的[HRP–H2O2]络合物可以氧化多种发色性氢供体。 AAT Bioquest提供了最大数量的HRP底物和缀合物。 HRP在许多不同的免疫化学应用（包括ELISA，免疫印迹和免疫组织化学）中被广泛用作免疫球蛋白的标记。 HRP可以通过几种不同的方法与抗体偶联，包括戊二醛，高碘酸盐氧化，通过二硫键以及氨基和硫醇定向的交叉油墨。迄今为止，我们的Buccutite HRP共轭技术是最有效的方法。 HRP是抗体最需要的标记，因为它是三种最流行的酶标记（HRP，碱性磷酸酶和B-半乳糖苷酶）中最小和最稳定的，其糖基化导致较低的非特异性结合。此纯化的辣根过氧化物酶（HRP）以无盐粉末形式冻干提供，用于重建并用于HRP检测或结合。

参考文献

Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: Food chemistry (2020): 126356

Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds.
Authors: Liu, Shan and Huang, Biyan and Zheng, Guiqin and Zhang, Peng and Li, Juying and Yang, Bo and Chen, Yantao and Liang, Li
Journal: International journal of biological macromolecules (2020): 814-822

An efficient methodology for the purification of date palm peroxidase: Stability comparison with horseradish peroxidase (HRP).
Authors: Saud Al-Bagmi, Moneera and Shahnawaz Khan, Mohd and Alhasan Ismael, Mohamad and Al-Senaidy, Abdulrahman M and Ben Bacha, Abir and Mabood Husain, Fohad and Alamery, Salman Freeh
Journal: Saudi journal of biological sciences (2019): 301-307

Sensitive sulfide ion detection by optofluidic catalytic laser using horseradish peroxidase (HRP) enzyme.
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Journal: Biosensors & bioelectronics (2017): 351-357

Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes for protein biomarker detection.
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Journal: Biosensors & bioelectronics (2016): 383-8

Bioconjugation of Antibodies to Horseradish Peroxidase (HRP).
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Journal: Methods in molecular biology (Clifton, N.J.) (2015): 43-50

Pb(2+)-introduced activation of horseradish peroxidase (HRP)-mimicking DNAzyme.
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Journal: Journal of hazardous materials (2007): 1073-8

FIA-near-infrared spectrofluorimetric trace determination of hydrogen peroxide using tricarchlorobocyanine dye (Cy.7.Cl) and horseradish peroxidase (HRP).
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Journal: Talanta (2006): 876-82

Studies on the oxidation reaction of tyrosine (Tyr) with H2O2 catalyzed by horseradish peroxidase (HRP) in alcohol-water medium by spectrofluorimetry and differential spectrophotometry.
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