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Products > Optimize Reagents > Optimize – Buffers > BICINE Buffer
BICINE Buffer
Applications
- Crystallization grade BICINE buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
BICINE
Synonyms: N,N-Bis(2-hydroxyethyl)glycine
C6H13NO4
Mr 163.17
CAS Number [150-25-4]
EC Number 205-755-1
Beilstein Registry Number 1769362
Merck 14,1201
RTECS MB9700000
MDL Number MFCD00004295
PubChem Substance ID 24891738
Purity ≥ 99.0%
pKa 8.3 at 20°C, 8.3 at 25°C
Useful pH Range 7.4-9.3
Titrate HR2-509 to useful pH range using NaOH
HR2-509 pH Range: 5.0 – 5.3 at 25°C
HR2-509 Measured Conductivity Range: 100.7 – 167.2 µS/cm at 25°C
HR2-509 Measured Refractive Index Range: 1.35769 – 1.35821 at 20°C
HR2-723 has been titrated to pH 9.0 using NaOH
HR2-723 Measured Conductivity Range: 23.9 – 25.0 mS/cm at 25°C
HR2-723 Measured Refractive Index Range: 1.36054 – 1.36122 at 20°C
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CAT NO
HR2-509
NAME
DESCRIPTION
100 mL
PRICE
$95.00
cart quote
CAT NO
HR2-723
NAME
DESCRIPTION
100 mL
PRICE
$95.00
cart quote
Support Material(s)
HR2-509 BICINE 1.0 M solution SDS
HR2-723 BICINE pH 9.0 1.0 M solution SDS
Titration TableCertificate Of Analysis
Related Item(S)
- Sodium hydroxide
- Individual StockOptions Bicine Reagents
- StockOptions Bicine Buffer Kit
References
1. Crystallization and preliminary X-ray diffraction studies of a fungal hydrolase from Ophiostoma novo-ulmi. M. N. Isupov, A. A. Brindley, E. J. Hollingsworth, G. N. Murshudov, A. A. Vagin and J. A. Littlechild. Acta Cryst. (2004). D60, 1879-1882.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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