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Products > Optimize Reagents > Optimize – Buffers > BIS-TRIS Buffer
BIS-TRIS Buffer
Applications
- Crystallization grade BIS-TRIS buffer for formulating screens or for optimization
Features
- Sterile filtered
- Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
BIS-TRIS
Synonyms: 2,2-Bis(hydroxymethyl)-2,2′,2″”-nitrilotriethanol, or 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, or Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane.
C8H19NO5
Mr 209.24
CAS Number [6976-37-0]
EC Number 230-237-7
Beilstein Registry Number 2205275
MDL Number MFCD00002853
Purity ≥ 98.0%
pKa = 6.4 at 20°C
pKa = 6.5 at 25°C
HR2-781 is titrated to pH 5.5 using HCl
Measured Conductivity Range of HR2-781: 38.4 – 43.1 mS/cm at 25°C
Measured Refractive Index of HR2-781: 1.37069 – 1.37150 at 20°C
HR2-783 is titrated to pH 6.5 using HCl
Measured Conductivity Range of HR2-783: 22.8 – 28.7 mS/cm at 25°C
Measured Refractive Index of HR2-783: 1.36817 – 1.36926 at 20°C
Al ≤0.0005%
Ca ≤0.0005%
Cl ≤0.05%
Cu ≤0.0005%
Fe ≤0.0005%
K ≤0.005%
Mg ≤0.0005%
NH4 ≤0.05%
Na ≤0.005%
Pb ≤0.001%
SO4 ≤0.05%
Zn ≤0.0005%
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CAT NO
HR2-781
NAME
DESCRIPTION
100 mL
PRICE
$155.00
cart quote
CAT NO
HR2-783
NAME
DESCRIPTION
100 mL
PRICE
$155.00
cart quote
Support Material(s)
HR2-781 BIS-TRIS pH 5.5 SDS
HR2-783 BIS-TRIS pH 6.5 SDSCertificate Of Analysis
Related Item(S)
- Hydrochloric acid
- StockOptions Bis-Tris Buffer Kit
- Individual StockOptions Bis-Tris Reagents
References
1. Atomic structure of the degraded procapsid particle of the bacteriophage G4: induced structural changes in the presence of calcium ions and functional implications. McKenna, R; Bowman, BR; Ilag, LL; Rossmann, MG; Fane, BA. J Mol Biol 256, 736- 750, 1996.
2. Expression, crystallization and preliminary X-ray diffraction study of FtsY, the docking protein of the signal recognition particle of E. coli. Montoya, G; Svensson, C; Luirink, J; Sinning, I. Proteins 28, 285- 288, 1997.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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