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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-211
NDSB-211
Applications
- Crystallization grade NDSB-211 for formulating screens or for optimization
Features
- Efficiently solubilizes proteins at non-denaturing concentrations
- Useful in preventing protein aggregation
- Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
- Protein specific additive for solubility and stability
Description
The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.
Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.
Typical useful NDSB concentration in protein sample is 0.5-1.0 M.
NDSB-211
Synonyms: Dimethyl(2-hydroxyethyl)ammonium propane sulfonate or Non detergent sulfobetaine 211
C7H17NO4S
Mr 211.30
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)
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CAT NO
HR2-793
NAME
DESCRIPTION
5 grams
PRICE
$116.00
cart quote
Support Material(s)
Certificate Of Analysis
References
1. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
2. Vuillard, L., et al. 1995. Biochem. J. 305-, 337.
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Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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