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Products > Optimize Reagents > Optimize – Cryoprotectants > Parabar 10312 (previously known as Paratone)
Parabar 10312 (previously known as Paratone)
Applications
- Oil based cryoprotectant
Features
- Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512
- Oil based cryoprotectant
Description
Sorry, Parabar 10312 is no longer available. We’ve left this page up for reference only. See HR2-861 Santovac Cryo Oil as a possible substitute.
Parabar 10312 is a viscous cryoprotectant for both small and large molecule crystallography.1
Mildly air-unstable, small molecule compounds can be coated with Parabar under an inert atmosphere. The Parabar protected crystal sample can be cryocooled in a chilled nitrogen gas stream. Parabar has also been used successfully as a cryoprotectant for biological macromolecule crystals. Used during cryocrystallography to displace and reduce the amount of water (mother liquor, reagent) on the crystal after the crystal is mounted in a cryoloop. Coating the crystal with Parabar can minimize evaporation from the crystal and reduce exposure and slow diffusion of air (oxygen) to the crystal.
HR2-643 Parabar 10312 is no longer available and has been discontinued effective May 11, 2021.
HR2-862 Parabar 10312 is supplied as 5 ml in an amber glass vial with glass applicator rod in screw cap lid.
The viscosity of Parabar can be reduced to improve handling with fragile needle and blade crystals by mixing 50:50 with Paraffin Oil.
Parabar is a blend of polyisobutylenes with a Staudinger molecular weight of about 20,000 and contains about 20% polymer.
Refractive index 1.48487 at 20°C.
Kinematic viscosity 641 cSt at 100°C. Kinematic viscosity ratio 2.05 at 100°C.
Clear to slightly hazy is appearance.
Synonyms used in the crystallographic literature: Paratone® N, Paratone® 8277, Infineum V8512.
Paratone® is a registered trademark of Chevron Corporation.
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Support Material(s)
HR2-862 Parabar 10312 User Guide
HR2-643 HR2-862 Parabar 10312 SDSCertificate Of Analysis
Related Item(S)
- Perfluoropolyether Cryo Oil
- Santovac Cryo Oil
References
1. H. Hope, Cryocrystallography of biological macromolecules: a generally applicable method. Acta Cryst. (1988) B44, 22-26.
2. Structure of the ligand-binding domain (LBD) of human androgen receptor in complex with a selective modulator LGD2226. F. Wang, X.- Liu, H. Li, K.- Liang, J. N. Miner, M. Hong, E. A. Kallel, A. van Oeveren, L. Zhi and T. Jiang. Acta Cryst. (2006). F62, 1067-1071.
3. H. Hope, Annu. Rev. Biophys. Chem. 1990 19:107-126.
4. S. Parkin and H. Hope, J. Appl. Cryst. (1998) pages 945-953.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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