VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
- 产品型号: PK-6100
- 简单描述
- VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)规格:1KIT试剂盒组份:2ml试剂A(Avidin DH溶液) 2ml试剂B(生物素化的酶) 1ml生物素化的二抗(1.5mg anti-IgG/0.5mg anti-IgM抗体/2.1mg通用抗体) 3ml阻断血清 其中standard试剂盒只含有试剂A和试剂B
详细介绍
VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
Vector Laboratories公司是世界著名的生物学检测试剂公司。1977年首先开发出了生物素-亲和素系统,该产品系列是检测方法上的重要革命。之后经过不懈的努力,VectorLaboratories公司发展出ABC技术(Avidin : Biotinylated Enzyme ComplexTechnology,亲和素-生物素-过氧化物酶复合物技术)并建立了著名的VECTASTAIN@ ABC系统,该系统被普遍视为目前zui灵敏、zui可靠与zui有效的染色系统,并被广泛应用于免疫组织化学、免疫电镜、原位杂交与凝集素化学中。而该系统仍在不断地发展,以满足广大研究人员的各种不同要求(如多抗原的标记检测)
VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
ABC技术利用生物素和卵白素具有极高亲和性的生物学特征,将卵白素和生物素化辣根酶按照一定的比例混合,形成ABC复合物。该法亦被称作三步法,*步为biotin化二抗和一抗结合,第二步为avidin(亲和素,A液)和二抗上的biotin结合,第三步为HRP偶联的biotin(B液)和avidin结合,而HRP再催化底物显色完成染色。
VECTOR免疫组化试剂盒VECTASTAIN ABC Kit(PK-6100)
Features:
- Advanced avidin/biotin technology: The Elite® ABC complex is smaller, very uniform, and highly active, allowing more accessibility for binding to a biotinylated target
- HIghest sensitivity, low background: The VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system. The Elite® ABC series is approximay 5 times more sensitive than the original VECTASTAIN® ABC Kit with the same low background.
- Cost effective: HIgher sensitivity leads to lower cost per slide
- Available without (“Standard” kit) or with biotinylated species-specific or universal secondary antibodies
- Available in ready-to-use formats: Prediluted, stabilized working solutions of Elite® ABC Kit reagents provide the same high sensitivity and low background as the traditional VECTASTAIN® Elite® ABC Kit reagents.