CST Anti-GFP （D5.1） XP抗体

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CST Anti-GFP （D5.1） XP抗体

  • 产品型号：  2956
  • 简单描述
  • CST Anti-GFP （D5.1） XP抗体
    Isotype：Rabbit IgG
    Applications：W IHC-P IF-IC F
    Reactivity：All
    Sensitivity：Transfected Only
详细介绍

CST Anti-GFP （D5.1） XP抗体

Specificity / Sensitivity

GFP （D5.1） XP® Rabbit mAb detects GFP-tagged proteins exogenously expressed in cells.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.

Western Blotting

CST Anti-GFP （D5.1） XP抗体

Western blot analysis of extracts from HCC827 cells, untransfected or GFP-transfected, using GFP （D5.1） XP® Rabbit mAb.

IHC-P （paraffin）

CST Anti-GFP （D5.1） XP抗体

Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected （left） or GFP-transfected （right）, using GFP （D5.1） XP® Rabbit mAb.

Flow Cytometry

CST Anti-GFP （D5.1） XP抗体

Flow cytometric analysis of HCC827 cells, untransfected （blue） or GFP-transfected （green）, using GFP （D5.1） XP® Rabbit mAb.

 

IF-IC

CST Anti-GFP （D5.1） XP抗体

Confocal immunofluorescent analysis of COS-7 cells expressing a GFP-tagged fusion protein （green） using GFP （D5.1） XP® Rabbit mAb （red）. Blue pseudocolor = DRAQ5® #4084 （fluorescent DNA dye）.

CST Anti-GFP （D5.1） XP抗体

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The green-fluorescent protein （GFP） functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization （1）.

  1. Miyawaki, A. et al. （2003） Curr Opin Chem Biol 7, 557-62.

Application References

  • Matheny, R.W. and Nindl, B.C. （2011） Endocrinology 152, 1923-34. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.