qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
- 产品型号: 59824
- 简单描述
- qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒对来自基因组DNA、福尔马林固定石蜡包埋(FFPE)样本、细胞或组织样本的DNA进行超快速、高效的亚硫酸氢盐转化
qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
- 优化的操作流程对胞嘧啶转化率超过99%
- 新型Bisulfite Solution可在少至30分钟内完成有效地亚硫酸氢盐转化
- 可用于基因组DNA、全血、培养细胞、组织样本和福尔马林固定石蜡包埋(FFPE)切片
- 快速、简洁的操作流程,有效转化和纯化DNA
- 独特的DNA Protect技术可防止DNA降解
EpiTect Fast Bisulfite Conversion Kits可在少至30分钟内将未甲基化的胞嘧啶高效转化成尿嘧啶,起始材料选择也有出色的灵活性。简化的样本裂解操作流程和直接的DNA亚硫酸氢盐转化使转化过程快速、省力,样本包括全血、培养细胞、组织样本、基因组DNA和福尔马林固定石蜡包埋(FFPE)切片。创新的DNA Protect技术确保有效的DNA变性而不会降解,得到高产量DNA和可重复性结果。此外,转化DNA可在少至10 µl洗脱缓冲液中洗脱,得到高浓度DNA。
qiagen EpiTect Fast DNA Bisulfite Kit快速亚硫酸氢盐转化试剂盒
Performance
高效亚硫酸氢盐转化
在甲基化分析中,对DNA亚硫酸氢盐处理,将未甲基化的胞嘧啶转化成胞嘧啶是zui关键的步骤,因为反应效率会对下游分析的可靠性造成很大影响。EpiTect Fast Bisulfite Conversion Kits含有的创新型Bisulfite Solution可进行超快速、高效的亚硫酸氢盐转化,甚至可处理难分析的样本,如来自女性样本的X染色体基因组DNA,因为2个X染色体中有一个是高度甲基化的(参见”Highly efficient cytosine conversion of X chromosome DNA”)。焦磷酸测序对胞嘧啶转化定量分析表明,与其他供应商的试剂盒相比,EpiTect Fast Bisulfite Conversion Kit的转化效果明显更好,特别是当起始材料的量很有限时(参见”Superior bisulfite conversion”)。
订购信息:
Cat No./ID: 59802 EpiTect Fast Bisulfite Kit (10) Log in Trial kit for 10 preps: Deparaffinization Solution, Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59824 EpiTect Fast DNA Bisulfite Kit (50) Log in For 50 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59826 EpiTect Fast DNA Bisulfite Kit (200) Log in For 200 preps: Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59864 EpiTect Fast LyseAll Bisulfite Kit (50) Log in For 50 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |
Cat No./ID: 59866 EpiTect Fast LyseAll Bisulfite Kit (200) Log in For 200 preps: Lysis Buffer, Proteinase K, Bisulfite Solution, DNA Protect Buffer, MinElute DNA Spin Columns, Carrier RNA, and Buffers |