Hampton Research蛋白结晶试剂盒
Products > Optimization Screens > StockOptions Buffer Kits > StockOptions pH Buffer Kit
StockOptions pH Buffer Kit
Applications
- Crystallization grade buffer stocks for crystal screening, optimization, & production
Features
- Titrated, preformulated, & ready-to-use buffers
- pH 2.2 – 11.0
- 1.0 M concentrated stocks (0.5 M for CAPSO and CAPS due to solubility limits)
- Citric acid, Sodium acetate trihydrate, Sodium citrate tribasic dihydrate, Sodium cacodylate trihydrate, HEPES sodium, Tris hydrochloride, CAPSO, and CAPS buffers
Description
StockOptions reagent kits are reagent tool boxes for the macromolecular crystal grower. They offer precisely formulated, high quality crystallization grade reagent stocks in convenient, cost-effective kits. The chemicals utilized in these kits are the same crystallization grade, ultra-high purity chemicals utilized in the Hampton Research kits such as Crystal Screen™ and Crystal Screen 2™. StockOptions reagents are carefully formulated under strict quality standards to ensure reliable performance and lot-to-lot consistency.
StockOptions pH is a convenient and complete kit with buffers pHed from 2.2 to 11.0 in increments of 0.2 pH units. Each reagent is available in convenient concentration, making crystal setups quick and easy. Gone is the tedious task of finding and sourcing reagents, as well as costly and time-consuming reagent formulation.
The generation of custom screens or optimized conditions now simply involves pipetting StockOptions pH reagents from one convenient kit. This portfolio of buffers between pH 2.2 and 11, along with wide arrays of salts, polymers, and organic solvents also stimulates creativity since all of the tools are readily and conveniently available. All one needs to do is select the reagent and pipet.
The convenience also reduces the chance of errors. Preformulated stocks remove calculation, measurement, and formulation errors. No more second guessing how the reagent was formulated, what specific chemical was used, when it was made, or how to precisely reproduce that reagent when it is gone and more reagent is required for additional setups.
StockOptions pH is cost-effective. When a StockOptions kit is purchased, one is using reagents as preformulated stocks in reasonable volumes. You buy only the reagents you need, not a large container of raw material that may sit out on shelves for years to come. When you need larger volumes, Hampton Research offers individual, preformulated, sterile filtered Optimize™ crystallization reagents which include salts, polymers, organics solvents, and buffers. Also, one can special order crystallization reagents through the Hampton Research Custom Shop. Optimize, Custom Shop, StockOptions, and all Hampton Research kits are synergistic research tools.
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CAT NO
HR2-241
NAME
DESCRIPTION
pH 2.2 to 11.0 in 0.2 pH unit increments
PRICE
$345.00
cart quote
Support Material(s)
HR2-241 StockOptions pH Documents
HR2-241 StockOptions pH SDSRelated Item(S)
- Individual StockOptions pH Reagents
References
1. Structure of the ribosomal interacting GTPase YjeQ from the enterobacterial species Salmonella typhimurium. D. K. Stammers et al. Acta Cryst. (2007). F63, 922-928
2. Increasing the size of microcrystals by fine sampling of pH limits. A. McPherson. J. Appl. Cryst. (1995). 28, 362-365.
3. Crystallization Optimum Solubility Screening: using crystallization results to identify the optimal buffer for protein crystal formation. B. Collins, R. C. Stevens and R. Page. Acta Cryst. (2005). F61, 1035-1038.
4. Optimum Solubility (OS) Screening: an efficient method to optimize buffer conditions for homogeneity and crystallization of proteins. Jancarik, J., Pufan, R., Hong, C., Kim, R., Kim, S.-H. Acta Cryst. D(60): 1670-1673.
5. Microdrop screening: A rapid method to optimize solvent conditions for NMR spectroscopy of proteins. Lepre, C. A., Moore, J. M. Journal of Biomolecular NMR, 12: 493-499, 1998.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA).
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