The NEBNext Ultra II Ligation Module has been optimized to ligate efficiently DNA adaptors compatible

with Illumina sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with

the NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546 ), and is part of the Ultra II DNA workflow which enables high yield preparation of high quality libraries from 500 pg to 1 µg of input DNA.

The NEBNext Ultra II Ligation Module is Designed for use with the Following:
 •  NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546)
 •  NEBNext Ultra II Q5® Master Mix (NEB #M0544)
 •  NEBNext Oligos for Illumina (NEB #E7335, #E7500, #E7710, #E7730, #E6609, #E7600 #E7535 , 

    NEB #E7350)

    NEBNext® Ligation Enhancer

    NEBNext® Ultra™ II Ligation Master Mix

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